Small: Collaborative Research: Programmed Cyclic Molecular Dancing on 2D Origami Lattices

小：合作研究：二维折纸晶格上的程序化循环分子跳舞

• 批准号: 1526650
• 负责人: Nadrian Seeman
• 金额: $ 25万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1526650&HistoricalAwards=false
• 关键词: Small; Collaborative; Research; Programmed; Cyclic

Molecular interactions can be seen as molecular communications and exchanges of information. In recent years the understanding of molecular information processing has been explored through DNA-based bottom-up self-assembly. These studies explore algorithmic, programmable molecular interactions including molecular devices. In this work, two PIs with complementary expertise, a chemist and a mathematician, join together to further the possibilities for programmed molecular interactions. The goal of this highly interdisciplinary project is to introduce environmental control within the DNA self-assembly process that will direct molecular interactions in a preprogrammed manner. It introduces a "clock" (an essential part of computation) that controls programmed oscillating attachments/detachments of different molecular species on a predesigned platform. The proposed project will enable self-assembly of a variety of preprogrammed molecular shapes from the same set of building blocks. These shapes could serve as scaffolds for nanoelectronic components, which if achieved, will allow for smaller, denser and faster computer hardware. Furthermore, programmed dynamical molecular interactions would be the first incarnation of nucleic acid robots (nubots) acting on a platform. Several undergraduates and at least two PhD graduate students (one in each institution) will receive training through this award. This project will prepare these individuals as unique interdisciplinary research scientists able to pursue studies that require broad scientific knowledge.The goal of this project is to introduce environmental control for periodic dynamical, molecular arrangements in molecular programming, to develop a method for molecular signal communication between different molecular species, and to use this method to obtain molecular arrangements that cycle in time. In particular the project will (1) develop molecular conditions for environmental control through laser illumination that will allow oscillating platform attachments/detachments between two different molecular floating tile species and (2) introduce algorithmic molecular interactions with oscillating dynamics between two molecular species by simulating a two-dimensional Game-of-Life-like dynamics on a two dimensional DNA origami array. The system has a platform consisting of a 2D DNA origami array whose tiles have signaling strands, and free floating tiles able to attach to their respective "identity" counterparts on the platform and to transmit signals to neighboring locations. The array tiles are arranged in a checker-board fashion such that in an alternate manner, at each cycle, one of the colors receives the floating tiles and computes the identity of the next cycle tiles of the other color tiles. The floating tiles are equipped with nano-particles whose exposure to appropriate laser wave length increases the local temperature and thereby can disassociate those floating tiles from the platform.

分子间的相互作用可以被看作是分子间的通讯和信息交换。近年来，分子信息处理的理解已经通过基于DNA的自底向上的自组装进行了探索。这些研究探索算法，可编程的分子相互作用，包括分子器件。 在这项工作中，两个具有互补专业知识的PI，一个化学家和一个数学家，共同努力进一步提高编程分子相互作用的可能性。这个高度跨学科的项目的目标是在DNA自组装过程中引入环境控制，以预先编程的方式指导分子相互作用。它引入了一个“时钟”（计算的重要组成部分），控制预先设计的平台上不同分子种类的编程振荡附件/determination。 拟议的项目将使自组装的各种预先编程的分子形状从同一套积木。这些形状可以作为纳米电子元件的支架，如果实现，将允许更小，更密集和更快的计算机硬件。此外，编程的动态分子相互作用将是核酸机器人（nubots）在平台上的第一个化身。一些本科生和至少两名博士研究生（每个机构一名）将通过该奖项接受培训。本项目的目的是将这些人培养成能够从事需要广泛科学知识的研究的独特的跨学科研究科学家。本项目的目标是在分子编程中引入周期性动态分子排列的环境控制，开发不同分子物种之间的分子信号通信方法，并使用该方法获得随时间循环的分子排列。特别是，该项目将（1）通过激光照明开发用于环境控制的分子条件，这将允许两种不同分子浮动瓦物种之间的振荡平台连接/分离，以及（2）通过模拟二维DNA折纸阵列上的二维生命游戏样动力学，引入两种分子物种之间的振荡动力学的算法分子相互作用。 该系统具有由二维DNA折纸阵列组成的平台，该二维DNA折纸阵列的瓦片具有信号链，以及自由浮动的瓦片，该自由浮动的瓦片能够附着到平台上的它们各自的“身份”对应物并将信号传输到相邻位置。阵列瓦片以棋盘的方式布置，使得在每个周期中，以交替的方式，颜色之一接收浮动瓦片并计算其他颜色瓦片的下一周期瓦片的身份。浮动瓦片配备有纳米颗粒，纳米颗粒暴露于适当的激光波长增加了局部温度，从而可以使那些浮动瓦片与平台分离。