MRI: Acquisition of a High-Resolution Mass Spectrometer

从重大研究仪器(MRI)和化学研究仪器和设施(CRIF)计划获得这一奖项后，夏威夷大学将获得一台集成的超高性能液相色谱仪(UHP-LC)和带有四极飞行时间(Q-TOF)的高分辨率质谱仪(HRMS)。该系统将用于分析从各种来源获得的材料的成分，包括从化学反应、天然产品和生物来源获得的样品。液相色谱系统(LC)将混合物分离成其单独的组分。质谱学(MS)然后电离这些组分，并通过测量离子的质量电荷比(m/z)来确定它们的质量。选定的配置将实现准确的质量测定，并有能力解析和表征复杂混合物的成分。这是一种广泛使用的分析工具，用于确定混合物或材料的组成。学生将在研究中使用这种仪器，并用先进的现代仪器对他们进行培训。实验室课程的学生将接受使用这一工具的培训，为他们进入研究生学习和化学及相关领域的职业生涯做好准备。该质谱学系统将用于如下领域的研究：(A)合成基于磷杂烯的配体及其相应的过渡金属络合物在小分子活化、均相催化和替代能源过程中的应用；(B)追求天然产物的全合成和开发新的成键方法；(C)研究自由基S-腺苷蛋氨酸酶产生C-S键的机制；(D)研究弹性蛋白蛋白；(E)开发膜蛋白表达和结晶技术；(F)开展生物活性小分子的全合成和合成；(G)鉴定复杂生物样品中的小分子。

With this award from the Major Research Instrumentation (MRI) and Chemistry Research Instrumentation and Facilities (CRIF) programs, the University of Hawaii will acquire an integrated ultra-high-performance liquid chromatograph (UHP-LC) and high-resolution mass spectrometer (HRMS) with quadrupole time-of-flight (Q-TOF). The system will be used to analyze the composition of materials obtained from various sources including samples obtained from chemical reactions, natural products and biological sources. The liquid chromatography system (LC) separates a mixture into its individual components. Mass Spectrometry (MS) then ionizes the components and determines their mass by measuring the mass to charge ratio (m/z) of the ions. The selected configuration will enable accurate mass determination, with the capability to resolve and characterize the components of complex mixtures. This is a widely used analytical tool to identify the composition of a mixture or material. The instrument will be used by students in their research, training them with sophisticated, modern instrumentation. Students in laboratory courses will be trained to use this tool preparing them for their advancement into graduate study and careers in chemistry and allied fields. The mass spectrometry system will be used in research including areas such as (a) synthesizing phosphaalkene-based ligands and the application of their corresponding transition metal complexes to problems in small molecule activation, homogeneous catalysis, and alternative energy processes; (b) pursuing total synthesis of natural products and the development of new bond-forming processes; (c) studying mechanisms of radical S-adenosylmethionine (SAM) enzymes that generate C-S bonds; (d) studying elastin protein; (e) developing technologies for membrane protein expression and crystallization; (f) developing total synthesis and synthesis of bioactive small molecules and (g) identifying small molecules in complex biological samples.