SBIR Phase II: Rapid detection of phages in microbial fermentation processes

SBIR 第二阶段：快速检测微生物发酵过程中的噬菌体

• 批准号: 1534756
• 负责人: Sudheendra Lakshmana
• 金额: $ 75万
• 依托单位: Sonanutech Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1534756&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Rapid; detection

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project is to reduce cost and loss of productivity due to bacteriophage (phage) contamination in fermentation of dairy and other products. Bacteriophage contamination is the leading cause of failure in fermentation processes used in the food and pharmaceutical industries. The risk of phage contamination in food fermentation processes, particularly in the dairy industry, is significantly enhanced due to a large diversity of suppliers of both the raw ingredient (milk) and the necessary microbial cultures. The current and emerging analytical technologies cannot rapidly detect phages in food materials such as milk. Thus, the combination of the risk of contamination, and the lack of rapid detection methods, results in a significant economic loss for the dairy industry. Early detection of phage contamination will permit early intervention in the fermentation process, which will lead to significant cost savings. In addition, the test could be extended to other food areas, pharmaceutical production, and biochemical production.The SBIR project addresses the need for rapid detection of phage contamination in a fermentation process through a novel photonic platform. The platform combines nanophotonics, microfluidics, and electrophoresis, and is based on discoveries made at University of California - Davis. The system is focused on the manipulation of charged phages with electrophoresis, and their accelerated amplification in bacteria. In addition, the system uses electrophoretic capture of phages into a nanophotonic crystal to achieve highly sensitive detection of the target phages. The salient features of the platform are: a) High-throughput, fast trapping of phages; b) excellent sensitivity and large dynamic range for quantification of phage concentration; and c) scalable design that can be produced at low cost.

该小企业创新研究 (SBIR) 项目更广泛的影响/商业潜力是降低乳制品和其他产品发酵过程中因噬菌体污染而造成的成本和生产力损失。 噬菌体污染是食品和制药行业发酵过程失败的主要原因。由于原材料（牛奶）和必要的微生物培养物的供应商多种多样，食品发酵过程（尤其是乳制品行业）中噬菌体污染的风险显着增加。当前和新兴的分析技术无法快速检测牛奶等食品材料中的噬菌体。因此，污染风险和快速检测方法的缺乏相结合，给乳制品行业带来了巨大的经济损失。 及早检测噬菌体污染将有助于及早干预发酵过程，从而显着节省成本。 此外，该测试还可以扩展到其他食品领域、药品生产和生化生产。SBIR 项目解决了通过新型光子平台快速检测发酵过程中噬菌体污染的需求。该平台结合了纳米光子学、微流体学和电泳技术，基于加州大学戴维斯分校的发现。该系统专注于通过电泳操纵带电噬菌体，及其在细菌中的加速扩增。 此外，该系统利用电泳捕获噬菌体到纳米光子晶体中，实现对目标噬菌体的高灵敏度检测。该平台的显着特点是： a) 高通量、快速捕获噬菌体； b) 噬菌体浓度定量的优异灵敏度和大动态范围； c) 可以低成本生产的可扩展设计。