Characterization of the molecular interplay between VASP, formins and their accessory proteins during filopodium formation

丝状足形成过程中 VASP、福明及其辅助蛋白之间分子相互作用的表征

• 批准号: 22176014
• 负责人: Professor Dr. Jan Faix
• 金额: --
• 依托单位: Institut für Biophysikalische Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/22176014?language=en
• 关键词: Characterization; molecular; interplay; between; VASP

Analysis of Dictyostelium formins showed that one of the regulated formins, dDia2, is specifically involved in the formation and maintenance of filopodia. These finger-like, highly dynamic cytoskeletal structures contain bundles of linear actin filaments and seem to be used by many cell types as sensing organs to explore environmental cues. Filopodia have also been implicated in cell motility as well as in cell-substrate adhesion, however, the molecular mechanisms underlying filopodia formation are still poorly understood. Our results clearly differ from published models of filopodia formation, which suggest that the Arp2/3 complex together with VASP play key roles during this process. Our data provide conclusive evidence for an alternative model in which the nucleating and uncapping activities of a formin and the bundling activity of VASP are required. The objective of this project is the detailed characterization of Diaphanous-related formin dDia2 and its accessory proteins in order to better understand the fundamental principles and mechanisms of filopodia formation at the molecular level shared across many species.

对盘基网柄菌福明斯的分析表明，一种受调节的福明斯 dDia2 专门参与丝状伪足的形成和维持。这些手指状、高度动态的细胞骨架结构包含线性肌动蛋白丝束，似乎被许多细胞类型用作探索环境线索的传感器官。丝状伪足也与细胞运动以及细胞-基质粘附有关，然而，丝状伪足形成的分子机制仍然知之甚少。我们的结果明显不同于已发表的丝状伪足形成模型，这表明 Arp2/3 复合体与 VASP 在此过程中发挥着关键作用。我们的数据为替代模型提供了确凿的证据，其中需要福尔明的成核和开盖活动以及 VASP 的成束活动。该项目的目标是详细表征透明相关的 formin dDia2 及其辅助蛋白，以便更好地了解许多物种共享的分子水平上丝状伪足形成的基本原理和机制。

项目成果

The application of the Cre-loxP system for generating multiple knock-out and knock-in targeted loci.

• DOI: 10.1007/978-1-62703-302-2_13
• 发表时间: 2013-01-01
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Faix, Jan;Linkner, Joern;Kimmel, Alan R
• 通讯作者: Kimmel, Alan R

A dual role for Rac1 GTPases in the regulation of cell motility

• DOI: 10.1242/jcs.089680
• 发表时间: 2012-01-15
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Filic, Vedrana;Marinovic, Maja;Weber, Igor
• 通讯作者: Weber, Igor

Filopodia formation induced by active mDia2/Drf3

• DOI: 10.1111/j.1365-2818.2008.02063.x
• 发表时间: 2008-09-01
• 期刊: JOURNAL OF MICROSCOPY-OXFORD
• 作者: Block, J.;Stradal, T. E. B.;Faix, J.
• 通讯作者: Faix, J.

Cofilin cooperates with fascin to disassemble filopodial actin filaments

• DOI: 10.1242/jcs.086934
• 发表时间: 2011-10-01
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Breitsprecher, Dennis;Koestler, Stefan A.;Faix, Jan
• 通讯作者: Faix, Jan