权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

EAGER: Therapeutic Protein Separations via Surface Isoelectric Focusing (sIEF)

EAGER：通过表面等电聚焦 (sIEF) 进行治疗性蛋白质分离

基本信息

批准号：
1548107
负责人：
Adrienne Minerick
金额：
$ 5万
依托单位：
Michigan Technological University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-15 至 2017-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1548107&HistoricalAwards=false
关键词：
EAGER Therapeutic Protein Separations via

项目摘要

1548107Michigan Technological UniversityMinerickThis one year EAGER will explore a new and potentially transformative approach to small volume protein separations called surface isoelectric focusing (sIEF). sIEF offers the potential for simpler, cheaper, quicker, and sensitive analysis by operating 100 times smaller than previous IEF techniques. This research will expand knowledge in isoelectric focusing to separate and identify amphoteric molecules, e.g., proteins, at very high resolution, which is fundamental to medical diagnostics of proteopathy diseases involving protein abnormalities and pharmaceutical screening of therapeutic proteins. IEF in larger scale slab gels is resource, labor, and time intensive being replaced clinically by smaller volume, genomic and affinity approaches. Glycoengineering of therapeutic proteins has been shown to improve molecular stability, regulate physicochemical and pharmacological properties, and improve pharmacokinetics with better absorption, longer circulation times, and decreased clearance rates. This work could produce the knowledge necessary to lead to the beginning of an sIEF tool which could interface seamlessly with therapeutic protein libraries. The result would be rapid screening and valuable charge and isoelectric point information to aid with the development of therapeutic proteins.

1548107密歇根理工大学Minerick这一年EAGER将探索一种新的和潜在的变革性的方法来分离小体积蛋白质，称为表面等电聚焦（sIEF）。sIEF提供了更简单，更便宜，更快，更灵敏的分析的潜力，操作比以前的IEF技术小100倍。这项研究将扩大等电聚焦的知识，以分离和鉴定两性分子，例如，蛋白质，这是涉及蛋白质异常的蛋白质病疾病的医学诊断和治疗性蛋白质的药物筛选的基础。大规模平板凝胶中的IEF是资源、劳动和时间密集型的，在临床上被较小体积的基因组和亲和方法所取代。治疗性蛋白质的糖工程化已显示出改善分子稳定性，调节物理化学和药理学性质，并改善药代动力学，具有更好的吸收、更长的循环时间和降低的清除率。这项工作可以产生必要的知识，导致sIEF工具的开始，可以与治疗性蛋白质文库无缝对接。其结果将是快速筛选和有价值的电荷和等电点信息，以帮助开发治疗性蛋白质。