SGER: Exploration and Quantification of Ion Gradients in a Capillary Microdevice

摘要提案编号：CTS-0636254主要研究者：Minerick，Adrienne R.附属机构： 密西西比州立大学提案标题： SGER：毛细管微器件中离子交换剂的探索和定量智力优势：开发易于筛选pH梯度微器件的工具将对学术界和工业界具有实际意义。这一信息加上越来越多的了解选择性离子运动及其对壁电荷的动态影响是学术和工业微器件研究人员的智力兴趣。PI的初步结果显示，在含有电极的2 mL样品威尔斯孔中pH值发生显著变化。下一步是直接测量微型装置通道内的任何pH梯度轮廓。一个直的，熔融石英毛细管微器件是一个理想的系统，进一步了解离子如何选择性地沿轴向移动的毛细管的长度，以及径向无论是朝向或远离毛细管壁。这项工作将比较直接测量数据与理论预测，并在必要时对模型进行修改。该项目将促进一个研究生和一个本科生的智力发展。更广泛的影响：拟议的研究将使确定pH值和其他离子梯度的微型设备与广泛的影响，开发一种工具，用于筛选微型设备的各种应用。这项技术还可以将等电聚焦技术带到纳升流体的规模。研究生和本科生的教育和专业发展将在这个为期一年的项目期间继续进行。成功的研究和教育技术将广泛传播给学术界和微型技术界。

ABSTRACTProposal Number: CTS-0636254Principal Investigator: Minerick, Adrienne R.Affiliation: Mississippi State UniversityProposal Title: SGER: Exploration and Quantification of Ion Gradients in a Capillary Microdevice Intellectual Merit: The development of tools to easily screen microdevices for pH gradients would be of practical interest to academic and industrial communities. This information coupled with a growing understanding of selective ion movement and its dynamic impact on wall charge is of intellectual interest to academic and industrial microdevice researchers. Preliminary results by the PI show pH changes significantly in 2 mL sample wells containing electrodes. The next step is to directly measure any pH gradient contours within the channels of the microdevice. A straight, fused silica capillary microdevice is an ideal system with which to further understand how ions selectively move axially down the length of the capillary as well as radially either toward or away from the capillary walls. This work will compare direct measurement data to theoretical predictions and develop modifications to models if necessary. This project will promote the intellectual development of a graduate and an undergraduate student.Broader Impacts: The proposed research would enable determination of pH and other ion gradients within microdevices with the broad impact of developing a tool for the screening of microdevices for various applications. This technology could also bring isoelectric focusing techniques to the scale of nanoliters of fluid. The educational and professional development of graduate and undergraduate students will continue during the duration of this one-year project. Successful research and educational techniques will be broadly disseminated to the academic, and microtechnological communities.