I-Corps Teams: Novel Rapid Non-destructive Cell Culture Platform

I-Corps Teams：新型快速无损细胞培养平台

• 批准号: 1551309
• 负责人: Nathan Gallant
• 金额: $ 5万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2017-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1551309&HistoricalAwards=false
• 关键词: Corps; Teams; Novel; Rapid; Non

This project aims to evaluate the commercial potential of a rapid non-destructive cell culture platform based on polymer textures that change shape by adjusting the temperature. Cell culture is a common activity in medicine and research in which cells are taken from a source, such as a patient, and put into a culture dish to multiply. A challenge in this activity is to remove the attached cells from the cell culture dish for further use. If cells remain attached for too long and overpopulate the surface they die, so they have to be regularly transferred to a new dish. The current most popular method of removing them from a dish uses a chemical dissolving agent which dissociates them but also damages or kills the cells. Afterward, cells have to be rinsed and need about a day to recover before they can be used or studied. It's only done this way because currently, there isn't a better alternative. The motivation for this project is driven by the need for greater reproducibility in laboratory testing of human cells. This technology is important in the advancement of stem cell research/therapies, eliminating animal testing for pharmaceuticals, cosmetics, and consumer products, as well as other medical-related areas which use culture of cells. The proposed technology is significant because it allows for a faster and non-destructive option for culturing cells as compared to current technologies available in the market place. To evaluate the commercial potential of the proposed technology, a rapid non-destructive cell culture platform, this team will engage in direct conversations with potential customers and trade partners to determine what is considered innovation in the cell culture market, identify a market-supported price to acquire this innovation, and develop an effective market activation strategy. The potential impact for this technology in several markets using cell culture is: $170 billion market for stem cell related activities and a $14.7 billion total cell culture market (2013) which is estimated to grow to $24.5 billion by 2018. Product development activities will consist of investigating rapid device fabrication, validation of release and viability with various cell types including stem cells, and developing non-confluent cell release. Development of this technology into a commercial product could positively impact the speed and accuracy with which cell culture is performed. The resultant advances in stem cell research and clinical tests can lead to faster diagnosis, cure, and management of disease in humans.

该项目旨在评估基于聚合物结构的快速非破坏性细胞培养平台的商业潜力，该平台可以通过调节温度来改变形状。细胞培养是医学和研究中的一项常见活动，从一个来源（如病人）取出细胞，并将其放入培养皿中繁殖。这项工作的一个挑战是将附着的细胞从细胞培养皿中取出以供进一步使用。如果细胞附着的时间过长，并且在表面上繁殖过多，它们就会死亡，所以它们必须定期转移到一个新的培养皿中。目前最流行的从培养皿中去除细胞的方法是使用一种化学溶解剂来分离细胞，但也会破坏或杀死细胞。之后，细胞必须被冲洗，大约需要一天的时间来恢复，然后才能使用或研究。之所以这样做，是因为目前还没有更好的选择。这个项目的动机是为了在人类细胞的实验室测试中获得更大的可重复性。这项技术对干细胞研究/治疗的进步非常重要，消除了药物、化妆品和消费品以及其他使用细胞培养的医疗相关领域的动物试验。与市场上现有的技术相比，所提出的技术具有重要意义，因为它允许更快和非破坏性的细胞培养选择。为了评估拟议技术的商业潜力，这是一个快速的非破坏性细胞培养平台，该团队将与潜在客户和贸易伙伴进行直接对话，以确定什么是细胞培养市场上的创新，确定获得这种创新的市场支持价格，并制定有效的市场激活策略。这项技术在使用细胞培养的几个市场中的潜在影响是：干细胞相关活动的市场为1700亿美元，总细胞培养市场为147亿美元（2013年），预计到2018年将增长到245亿美元。产品开发活动将包括研究快速设备制造，包括干细胞在内的各种细胞类型的释放和生存能力验证，以及开发非融合细胞释放。将这项技术发展为商业产品可能会对细胞培养的速度和准确性产生积极影响。由此产生的干细胞研究和临床试验的进步可以更快地诊断、治疗和管理人类疾病。