CAREER: Development of an In Cell Footprinting Method for the Analysis of Protein Structure

职业:开发用于分析蛋白质结构的细胞内足迹方法

基本信息

  • 批准号:
    1552509
  • 负责人:
  • 金额:
    $ 109.89万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-06-01 至 2016-12-31
  • 项目状态:
    已结题

项目摘要

Membrane proteins are involved in many important cellular functions. Despite their biological importance, studies with membrane proteins have been hampered by difficulties involved in purification of these proteins. Since most studies with membrane proteins require that these proteins to be in a membrane-like environment, a variety of membrane mimicking systems have been developed, which adds another layer of complexity. This project will develop a novel and innovative approach that will significantly advance knowledge in the field of membrane proteins by providing a method that can characterize them in their native lipid environment in living cells. The method will first be developed using a well-characterized cytostolic protein as a model system and then the membrane protein angiomotin will be used as a model system for the study of transmembrane proteins. The project will provide a gateway to understanding the physiology and pathophysiology of membrane trafficking. This project will provide training for students from Historically Black Colleges and Universities in an eight week undergraduate summer program to provide STEM research opportunities. These students will be trained in consecutive summers and become mentors in their last year. The project will also provide training to pre-and post-doctoral students.The project focuses on the development of an in cell protein footprinting method coupled with mass spectrometry for the characterization of protein interactions inside the cell. A major hindrance in studying protein-lipid interactions is simulating the membrane environment of the cell. The approach this project develops will lead significant advances in studies of membrane proteins by providing a method that can characterize them in their native cellular environment. The method, which uses "caged hydrogen peroxide", will first be developed using a well-characterized cytosolic protein as a model system. Parameters of the method will be systematically optimized and quantitative benchmarks will be used to determine success. Statistical analysis will be used to validate the significance in observed changes in protein modification. Then the membrane protein angiomotin will be used as a model system for the study of transmembrane proteins. This project will result in the development of a novel analytical method for the study of proteins in the cellular environment in living cells. The method will allow for the study of membrane proteins that cannot be studied by currently available structural methods and provide new insight into membrane trafficking in cellular environment.
膜蛋白参与许多重要的细胞功能。 尽管它们的生物学重要性,膜蛋白的研究已被这些蛋白质的纯化所涉及的困难所阻碍。 由于大多数关于膜蛋白的研究都要求这些蛋白质处于膜样环境中,因此开发了多种膜模拟系统,这增加了另一层复杂性。该项目将开发一种新颖的创新方法,通过提供一种可以在活细胞中的天然脂质环境中表征膜蛋白的方法,显着推进膜蛋白领域的知识。该方法将首先开发使用一个良好的特性的细胞膜蛋白作为模型系统,然后膜蛋白angiomotin将被用作跨膜蛋白的研究模型系统。该项目将提供一个网关,了解膜贩运的生理学和病理生理学。该项目将在为期八周的本科暑期课程中为历史上黑人学院和大学的学生提供培训,以提供STEM研究机会。 这些学生将在连续的夏天接受培训,并在最后一年成为导师。该项目还将为博士生和博士后学生提供培训,重点是开发一种细胞内蛋白质足迹法,结合质谱法,用于表征细胞内蛋白质相互作用。研究蛋白质-脂质相互作用的一个主要障碍是模拟细胞的膜环境。该项目开发的方法将通过提供一种可以在其天然细胞环境中表征膜蛋白的方法,从而在膜蛋白的研究中取得重大进展。 该方法,它使用“笼过氧化氢”,将首先开发使用一个良好的特点胞质蛋白作为模型系统。该方法的参数将得到系统优化,并将使用定量基准来确定成功与否。将使用统计分析来验证观察到的蛋白质修饰变化的显著性。膜蛋白血管动蛋白将作为研究跨膜蛋白的模型系统。该项目将导致开发一种新的分析方法,用于研究活细胞中细胞环境中的蛋白质。该方法将允许膜蛋白的研究,不能通过目前可用的结构方法进行研究,并提供新的见解膜运输细胞环境。

项目成果

期刊论文数量(0)
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Lisa Jones其他文献

Mood episodes in pregnancy and risk of postpartum recurrence in bipolar disorder: The Bipolar Disorder Research Network Pregnancy Study.
怀孕期间的情绪发作和双相情感障碍产后复发的风险:双相情感障碍研究网络妊娠研究。
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    6.6
  • 作者:
    A. Perry;K. Gordon;A. Di Florio;N. Craddock;Lisa Jones;I. Jones
  • 通讯作者:
    I. Jones
Infrared Thermal Imaging Has the Potential to Reduce Unnecessary Surgery and Delays to Necessary Surgery in Burn Patients
红外热成像有可能减少烧伤患者不必要的手术和必要手术的延误
  • DOI:
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    1.4
  • 作者:
    A. Singer;P. Relan;L. Beto;Lisa Jones;S. Sandoval;R. Clark
  • 通讯作者:
    R. Clark
NRG1 gene in recurrent major depression: No association in a large‐scale case–control association study
NRG1 基因与复发性重度抑郁症:大规模病例对照关联研究中没有关联
TU25. POLYGENIC PREDICTION OF LONGITUDINAL COURSE IN BIPOLAR DISORDER
  • DOI:
    10.1016/j.euroneuro.2021.08.028
  • 发表时间:
    2021-10-01
  • 期刊:
  • 影响因子:
  • 作者:
    Amedeo Primerano;Katherine Gordon-Smith;Lisa Jones;Valentina Escott-Price;Nick Craddock;Ian Jones;Arianna Di Florio
  • 通讯作者:
    Arianna Di Florio
Clinical Challenges and Opportunities of Bipolar Disorder Genomics
  • DOI:
    10.1016/j.biopsych.2021.02.175
  • 发表时间:
    2021-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Arianna Di Florio;Kath Gordon-Smith;Lisa Jones;Nick Craddock;Ian Jones
  • 通讯作者:
    Ian Jones

Lisa Jones的其他文献

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{{ truncateString('Lisa Jones', 18)}}的其他基金

CAREER: Development of an In Cell Footprinting Method for the Analysis of Protein Structure
职业:开发用于分析蛋白质结构的细胞内足迹方法
  • 批准号:
    1701692
  • 财政年份:
    2016
  • 资助金额:
    $ 109.89万
  • 项目类别:
    Continuing Grant

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CAREER: Development of an In Cell Footprinting Method for the Analysis of Protein Structure
职业:开发用于分析蛋白质结构的细胞内足迹方法
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    1701692
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    2016
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