CAREER: Time-domain encoded optofluidics for highly multiplexed pathogen detection

PI：Issadore，David提案：1554200该提案的目标是开发一种新的技术平台，用于在微流控芯片上读出基于多个荧光微滴的分析。这项拟议的技术可能会在医疗诊断、环境监测和生化战防御方面产生重大影响。该项目教育计划经过深思熟虑，包括开发一门高级设计课程，内容是在服务不足的国家为农村医疗提供适当的护理诊断。关键创新是对通过微流控通道的液滴产生的荧光信号进行幅度调制编码，每个通道使用独特的微图案(条形码)掩膜。这可能会解决单独检测每个液滴的问题，这非常耗时。相反，所有的液滴都是并行检测的，然后根据条形码进行信号分解。这是优雅和创新的。公安部建议采用水中病原体条形码检测装置。这显然是一个非常重要的问题，特别是在发展中世界的实地条件下。

AbstractPI: Issadore, David Proposal: 1554200The objective of this proposal is to develop a new technological platform for reading out multiple fluorescence droplet based assays on microfluidic chips. The proposed technology could have significant impact in medical diagnostics, environmental surveillance, and bio/chemical warfare defense. The project educational program is well thought out and includes development of a senior design course on the appropriate point of care diagnostics for rural medicine in underserved countries.The key innovation is amplitude modulated encoding of the fluorescence signal generated by a droplet passing through a microfluidic channel using a distinct micro-patterned (barcoded) mask for each of the channels. This may solve the problem of detecting each droplet individually, which is very time consuming. Instead, all droplets are detected in parallel followed by signal decomposition based on the barcode. This is elegant and innovative. The PI proposes to adopt the device for barcode detection of pathogens in water. This is clearly a problem of very high importance, especially in field conditions in the developing world.