CAREER: Time-domain encoded optofluidics for highly multiplexed pathogen detection

职业：用于高度多重病原体检测的时域编码光流控

• 批准号: 1554200
• 负责人: David Issadore
• 金额: $ 51万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1554200&HistoricalAwards=false
• 关键词: CAREER; Time; domain; encoded; optofluidics

AbstractPI: Issadore, David Proposal: 1554200The objective of this proposal is to develop a new technological platform for reading out multiple fluorescence droplet based assays on microfluidic chips. The proposed technology could have significant impact in medical diagnostics, environmental surveillance, and bio/chemical warfare defense. The project educational program is well thought out and includes development of a senior design course on the appropriate point of care diagnostics for rural medicine in underserved countries.The key innovation is amplitude modulated encoding of the fluorescence signal generated by a droplet passing through a microfluidic channel using a distinct micro-patterned (barcoded) mask for each of the channels. This may solve the problem of detecting each droplet individually, which is very time consuming. Instead, all droplets are detected in parallel followed by signal decomposition based on the barcode. This is elegant and innovative. The PI proposes to adopt the device for barcode detection of pathogens in water. This is clearly a problem of very high importance, especially in field conditions in the developing world.

摘要PI：Issadore，大卫提案：1554200该提案的目的是开发一种新的技术平台，用于在微流控芯片上阅读基于多个荧光液滴的分析。这项技术可能会对医疗诊断、环境监测和生物/化学战防御产生重大影响。该项目的教育计划是经过深思熟虑的，包括为服务水平低下的国家的农村医学开发一个关于适当的护理点诊断的高级设计课程。关键的创新是对通过微流体通道的液滴产生的荧光信号进行调幅编码，每个通道使用不同的微图案（条形码）掩模。这可以解决单独检测每个液滴的问题，这是非常耗时的。相反，并行检测所有液滴，然后基于条形码进行信号分解。这是优雅和创新。主要研究者建议采用该设备对水中病原体进行条形码检测。这显然是一个非常重要的问题，特别是在发展中国家的实地条件下。