Selective multifunctionalization of antibody fragments and antibodies by enzyme-mediated orthogonal ligations

通过酶介导的正交连接对抗体片段和抗体进行选择性多功能化

• 批准号: 223116737
• 负责人: Professor Dr. Ulf Diederichsen (†)
• 金额: --
• 依托单位: Institut für Organische und Biomolekulare Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/223116737?language=en
• 关键词: Selective; multifunctionalization; antibody; fragments; antibodies

Since decades antibodies are invaluable tools for various applications mainly for use in diagnostics and therapy. They are target-specific and effective, stable and can be produced in large amounts. All antibodies are composed of two functional regions, namely the Fab and the Fc region. The Fab region is required for antigen recognition, while the Fc region couples the antibody to secondary immune effector functions. In the proposed project, the full length antibody as well as the antibody Fc region will be used as a scaffold for the display of bioactive molecules. The main objective is to apply bioorthogonal chemical and enzyme-mediated ligation strategies that allow for selective endowment of antibodies with different ligands to site-specifically introduce cell recognition modules, toxic payloads, or compounds that mediate the release of bioactive molecules into the cytosol of a target cell. Multiple functionalization of antibodies and antibody fragments inherits a plethora of potential future applications in cell biology, biotechnology, and medicine.

几十年来，抗体是各种应用的宝贵工具，主要用于诊断和治疗。它们是针对特定目标和有效的，稳定，可以大量生产。所有抗体都由两个功能区组成，即Fab和Fc区。Fab区是识别抗原所必需的，而Fc区则将抗体与次级免疫效应器功能偶联。在拟议的项目中，全长抗体以及抗体Fc区域将被用作展示生物活性分子的支架。主要目标是应用生物正交化学和酶介导的连接策略，允许选择性地赋予具有不同配体的抗体，以定点地引入细胞识别模块、有毒有效载荷或介导生物活性分子释放到靶细胞胞浆中的化合物。抗体和抗体片段的多功能化在细胞生物学、生物技术和医学领域具有广泛的潜在应用前景。