CSBR-Ownership Transfer: Curate, Preserve and Transfer Agriculturally and Environmentally Significant Microorganisms to established laboratories equipped for preservation and distr

该项目的目标是将目前位于夏威夷的太平洋细菌收集中心的大量植物相关细菌的培养物重新激活并转移到美国大陆的一个已建立的实验室，在那里细菌可以安全储存，重新培养并分发给全国的科学家。该藏品包括40多年来从世界各地的起源和栖息地收集的6000多种细菌菌株，是微生物学家、教育工作者和农业和食品行业专业人士的国宝。具有历史价值的微生物种质将被记录和保存。该收藏是不同学科科学家的国家资源，支持分类学、基因组学、检测技术和法医流行病学方面的当代合作研究。该集合的规模，新颖性和多样性允许在以前不可能的规模上对特征良好的参考菌株进行数据分析。这将是鉴定和表征与患病/受损贸易货物有关的微生物的宝贵资源，并将增加有抱负的微生物分类学家的教育和培训机会。通过使工业界和非营利性生物研究界都能以最低的成本获得选定的菌株，从位于中心的实验室获得这一庞大的菌株集合符合国家利益。细菌菌株将被活化、注释并准备转移到位于印第安纳州的Agdia公司的指定工作人员手中。每个培养物的纯度将被确认，重复将被消除，每个菌株的数据将被添加到一个统一的现代化数据库中。该项目的重点将放在通过对多种细菌基因的DNA序列分析确定的代表遗传细菌多样性的菌株的转移、分子诊断测试的开发以及最初在夏威夷用于筛选杂交瘤细胞系以生产病原体特异性单克隆抗体的菌株。描述遗传特征和种群多样性的出版物将在数据库中引用。剩余的菌株将在三年内进行组织、验证和记录，允许总共转移多达6000株。建立一个现代化的统一数据库，其中包括单个菌株的相关数据，是一项重大任务，它将使研究人员、学生和管理人员能够在要求培养之前做出明智的选择。用户为培养、运输和遵守联邦和州法规而支付的费用将带来持续的支持。参考菌株的备份存储将确保该菌株的长期完整性，并将作为一种宝贵的科学资源。每种菌株的特征目前正在夏威夷大学维护的数据库中进行组织。项目完成后，每种培养的数据将被转移到Agdia， Inc.，使公众可以访问（www.agdia.com）。

The goal of this project is to revitalize and transfer a large culture collection of plant-associated bacteria from the Pacific Bacterial Collection, which is currently located in Hawaii to an established laboratory on the U.S. mainland where the bacteria can be safely stored, re-cultured and distributed to scientists nationwide. The collection consists of more than 6,000 bacterial strains collected over a 40 year period from worldwide origins and habitats and represents a national treasure for microbiologists, educators, and professionals in agricultural and food industry. Historically valuable microbial germplasm will be documented and preserved. The collection is a national resource for scientists in diverse disciplines and supports collaborative contemporary research on taxonomy, genomics, detection technology and forensic epidemiology. The collection's size, novelty, and diversity permits data analysis of well-characterized reference strains on a scale not previously possible. This will be a valuable resource for identifying and characterizing microbes associated with diseased/compromised trade goods and will enhance educational and training opportunities for aspiring microbial taxonomists. Access to this large collection of strains from a centrally-located laboratory serves the national interest by enabling both industry and the non-profit biological research community to access selected strains at a minimal cost.Bacterial strains will be revitalized, annotated and prepared for transfer to designated staff at Agdia, Inc., located in Indiana. Purity of each culture will be confirmed, duplicates will be eliminated and data for each strain will be added to a unified and modernized database. The focus of this project will be on the transfer of strains that represent genetic bacterial diversity as determined by DNA sequence analysis of multiple bacterial genes, development of molecular diagnostic tests, and strains originally used in Hawaii to screen hybridoma cell lines for production of pathogen-specific monoclonal antibodies. Publications describing genetic characteristics and population diversity will be referenced in the database. Remaining strains will be organized, validated and documented over a three year period allowing total transfer of up to 6,000 strains. Creation of a modernized unified database, which includes relevant data for individual strains is a major undertaking that will enable researchers, students and regulatory personnel to make informed selections before requesting a culture. User fees for culturing, shipping and compliance with federal and state regulations will result in sustained support. The back-up storage of reference strains will ensure the long-term integrity of the collection and will serve as a valuable scientific resource. Characteristics of each strain are currently being organized into a database maintained at the University of Hawaii. After the project is completed the data accompanying each culture will be transferred to Agdia, Inc. enabling public access (www.agdia.com).