CSBR: Ownership Transfer of University of Alabama Chytrid Culture Collection to University of Michigan

CSBR：阿拉巴马大学 Chytrid 培养物保藏中心的所有权转让给密歇根大学

基本信息

批准号：
1910720
负责人：
Timothy James
金额：
$ 7.94万
依托单位：
Regents of the University of Michigan - Ann Arbor
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2021-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1910720&HistoricalAwards=false
关键词：
CSBR Ownership Transfer University Alabama

项目摘要

This project will secure an endangered collection of about 450 living strains of fungi from a group known as chytrids. The collection was developed by Dr. Martha Powell and Dr. Peter Letcher at the University of Alabama (UA) over the last 42 years. It served as the biological material that supported over 100 scientific papers on chytrids and contains unique strains unknown from any other collection. Chytrids are a poorly studied group which require unique conditions for their isolation and maintenance, and a large portion of known diversity of the group is represented in this collection. Although these microscopic fungi reproduce with a swimming spore (zoospore) they occur in soils as well as in fresh and saline waters; they fill important ecological roles as parasites and also as decomposers of unique material such as pollen, hair, and insect exoskeletons. The UA chytrid collection will be combined with a previously secured collection from the University of Maine into a joint collection termed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM). The CZEUM collection is being developed in order to serve as a repository for chytrid diversity in support of research on chytrid fungi by the global scientific community. This project has four primary goals. The first goal is to complete a physical transfer of the UA chytrid collection to the cryopreservation facility at the University of Michigan Research Museums Complex where it will be stored in the vapor of liquid nitrogen at -193 degrees Celsius. The second goal is to test the viability of each of the cultures in the collection and refreeze viable material in the liquid nitrogen freezers. Previously most samples had been stored in an ultralow freezer, which can lead to deterioration of viability. A third goal is to cryopreserve the remaining five persent of the collection for which cryopreservation had never been attempted. The final goal of the project is to characterize the strains using ribosomal RNA genes in order to provide an estimate of the true phylogenetic diversity of the collection. This latter goal involves sequencing small subunit, large subunit, and the internal transcribed spacer region of the ribosomal RNA using Oxford Nanopore Technology for strains in which this data is lacking. Successfully completed, this project will secure this unique reference collection and will enhance infrastructure for research on chytrid fungi at the University of Michigan and other institutions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将获得一个被称为Chytrids的小组的约450种真菌的濒危收藏。该收藏是在过去的42年中由阿拉巴马大学（UA）的玛莎·鲍威尔（Martha Powell）和彼得·莱彻（Peter Letcher）博士开发的。它充当了生物学材料，该材料支持了100多篇有关chytrids的科学论文，并包含其他任何收藏中未知的独特菌株。 Chytrids是一个研究良好的群体，需要独特的隔离和维护条件，并且该集合中代表了该组已知多样性的很大一部分。尽管这些微观真菌用游泳孢子（zoo孢子）繁殖，它们出现在土壤以及新鲜和盐水中。它们作为寄生虫的重要生态作用，也充当了独特材料的分解器，例如花粉，头发和昆虫外骨骼。 UA Chytrid系列将与先前从缅因州大学获得的保证收藏合并为一个联合收藏，称为密歇根大学（Czeum）的Zoosporic Eufungi收藏。正在开发Czeum Collection，以作为全球科学界支持Chytrid多样性的存储库。该项目有四个主要目标。第一个目标是完成将UA Chytrid收藏品的物理转移到密歇根大学研究博物馆综合大楼的冷冻保存设施，该设施将存储在-193摄氏度的液氮蒸气中。第二个目标是测试集合中每种培养物的生存能力，并在液氮冷冻器中重新冷冻可行的材料。以前，大多数样品已存储在超冰柜中，这可能导致生存能力恶化。第三个目标是冷冻保存从未尝试过冷冻保存的剩余五个景点。该项目的最终目标是使用核糖体RNA基因来表征菌株，以便估算集合的真正系统发育多样性。后一个目标涉及使用牛津纳米孔技术对核糖体RNA的小亚基，大亚基和内部转录的间隔区进行测序，以解决该数据所缺乏的菌株。该项目成功完成后，将确保此独特的参考集合，并将增强密歇根大学和其他机构的chytrid真菌研究基础架构。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子优点和更广泛的影响审查标准来通过评估来通过评估来支持的。