CSBR: Ownership Transfer of University of Maine Chytrid Fungal Collection to University of Michigan

CSBR:缅因大学 Chytrid 真菌收藏的所有权转让给密歇根大学

基本信息

项目摘要

This project will secure an endangered collection of approximately 500 diverse, living strains of a fungal group known as chytrids. As the world's largest collection of chytrids, it serves as a resource to the research community by providing cultures to biologists in both academic and industrial settings, resulting in biological insights found in over 200 published articles. Although these microscopic fungi reproduce with a swimming spore they occur in soils as well as in fresh and saline waters; they fill important ecological roles as parasites and also by degrading substrates such as pollen and other plant material, insect exoskeletons, hair, etc. Chytrids are notorious for their role in the global decline of amphibians; the collection contains over 180 strains of the amphibian pathogen from around the world. This collection was developed and maintained by Joyce Longcore at the University of Maine without institutional support for more than 25 years. To safeguard the chytrid collection, it will be moved to the University of Michigan's recently remodeled Research Museums Complex where it will be stored in a state-of-the-art cryopreservation facility and managed by the University's Herbarium unit. The collection will be a resource for the scientific community involved in research on chytrids and their function in terrestrial and aquatic systems.Chytrid fungi require special growth and maintenance conditions, because they are aquatic fungi that reproduce with flagellated, wall-less zoospores. Most of the University of Maine collection is cryopreserved, making inter-institutional transfer in a frozen state possible, however, approximately 12% of the collection is routinely transferred in liquid cultures, because it has not been amendable to freezing. The first goal of the project is to move the collection in Maine to the Research Museums Complex in Ann Arbor where fungal strains will be stored in liquid nitrogen freezers. Cryopreservation in the vapor of liquid nitrogen stabilizes samples at -193 degrees Celsius; because inadvertent exposure to temperatures above -80 affects the viability of frozen cultures, storage in liquid nitrogen is safer than storage in typical ultralow freezers with electrical compressors. A second goal of this project is to develop methods to freeze and secure the remaining 12% of strains that have been resistant to cryopreservation. To verify taxonomic assignments of the culture collection and to better characterize its contents, DNA barcoding will be performed by sequencing and making accessible the 18S and ITS ribosomal RNA gene regions. Finally, this project will transform the collection into a public resource by making additional data about the strains accessible online and improving the distribution and maintenance of the cultures.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该项目将确保一个濒危的收集约500种不同的,活的菌株的真菌群称为壶菌。 作为世界上最大的壶菌群,它通过向学术和工业环境中的生物学家提供培养物来为研究界提供资源,从而在200多篇已发表的文章中发现生物学见解。 尽管这些微小的真菌以游动的孢子繁殖,但它们存在于土壤以及淡水和盐水沃茨中;它们作为寄生虫以及通过降解诸如花粉和其他植物材料、昆虫外骨骼、毛发等基质来发挥重要的生态作用。收集了来自世界各地的180多种两栖类病原体。 这个收藏是由缅因州大学的乔伊斯·朗科在没有机构支持的情况下开发和维护的,超过25年。 为了保护壶菌的收藏,它将被转移到密歇根大学最近改造的研究博物馆综合大楼,在那里它将被储存在一个最先进的冷冻保存设施中,并由大学的植物标本室管理。这些标本将成为科学界研究壶菌及其在陆地和水生系统中功能的资源。壶菌真菌需要特殊的生长和维护条件,因为它们是水生真菌,用有鞭毛的无壁游动孢子繁殖。缅因州大学的大部分藏品都是冷冻保存的,这使得在冷冻状态下的机构间转移成为可能,然而,大约12%的藏品是在液体培养物中常规转移的,因为它不能冷冻。该项目的第一个目标是将缅因州的收藏品转移到安阿伯的研究博物馆综合体,在那里真菌菌株将被储存在液氮冷冻机中。 液氮蒸气中的冷冻保存使样品在-193摄氏度下稳定;由于无意中暴露于-80以上的温度会影响冷冻培养物的活力,因此在液氮中储存比在带有电动压缩机的典型超低温冷冻机中储存更安全。 该项目的第二个目标是开发冷冻和保护剩余的12%耐低温保存的菌株的方法。 为了验证培养物收集的分类学分配并更好地表征其内容物,将通过测序进行DNA条形码化,并使18 S和ITS核糖体RNA基因区域可访问。 最后,该项目将通过在线获取有关菌株的额外数据并改善培养物的分发和维护,将收集的菌株转化为公共资源。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Timothy James其他文献

Mycoloopの先へ、これから挑戦したいこと
除了 Mycoloop,我下一步想尝试什么
  • DOI:
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Hans-Peter Grossart;Christian Wurzbacher;Timothy James;Maiko Kagami;鏡味麻衣子;鏡味麻衣子;鏡味麻衣子
  • 通讯作者:
    鏡味麻衣子
Open Circuit Potential as a Tool for the Assessment of Binding Kinetics and Reagentless Protein Quantitation.
开路潜力作为评估结合动力学和无试剂蛋白质定量的工具。
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    M. Sharafeldin;Timothy James;Jason J. Davis
  • 通讯作者:
    Jason J. Davis
Evaluating Self-paced Computational Notebooks vs. Instructor-Led Online Lectures for Introductory Computer Programming
评估自定进度的计算笔记本与讲师指导的计算机编程入门在线讲座
The Appeal to Law to Provide Public Answers to Bioethical Questions: It All Depends What Sort of Answers You Want
  • DOI:
    10.1007/s10728-007-0074-4
  • 发表时间:
    2008-01-24
  • 期刊:
  • 影响因子:
    1.600
  • 作者:
    Timothy James
  • 通讯作者:
    Timothy James

Timothy James的其他文献

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{{ truncateString('Timothy James', 18)}}的其他基金

PurSUiT: Biodiversity exploration and phylogenetic systematics of the zoosporic fungal phylum Blastocladiomycota
追求:游动孢子真菌门芽生菌门的生物多样性探索和系统发育系统学
  • 批准号:
    2403677
  • 财政年份:
    2024
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
CSBR: Ownership Transfer of University of Alabama Chytrid Culture Collection to University of Michigan
CSBR:阿拉巴马大学 Chytrid 培养物保藏中心的所有权转让给密歇根大学
  • 批准号:
    1910720
  • 财政年份:
    2019
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
PurSUiT: Discovery and phylogenetic analysis of chytrid fungi using cultures and single cells
追求:利用培养物和单细胞发现壶菌并进行系统发育分析
  • 批准号:
    1929738
  • 财政年份:
    2019
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
DISSERTATION RESEARCH: Evolutionary Consequences of Pathogen Strain Competition in an Emerging Fungal Disease
论文研究:新兴真菌病中病原体菌株竞争的进化后果
  • 批准号:
    1601259
  • 财政年份:
    2016
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
Collaborative Research: The Zygomycetes Genealogy of Life (ZyGoLife)- the conundrum of Kingdom Fungi
合作研究:接合菌生命谱系(ZyGoLife)——真菌王国的难题
  • 批准号:
    1441677
  • 财政年份:
    2015
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
Digitization TCN: Collaborative: The Microfungi Collections Consortium: A Networked Approach toDigitizing Small Fungi with Large Impacts on the Function and Health of Ecosystems
数字化 TCN:协作:微型真菌收藏联盟:对对生态系统功能和健康产生重大影响的小型真菌进行数字化的网络方法
  • 批准号:
    1502703
  • 财政年份:
    2015
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
Unveiling the diversity and ecological role of the obligate parasitic fungi in phylum Cryptomycota
揭示隐菌门专性寄生真菌的多样性和生态作用
  • 批准号:
    1354625
  • 财政年份:
    2014
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Continuing Grant
Digitization TCN: Collaborative: The Macrofungi Collection Consortium: Unlocking a Biodiversity Resource for Understanding Biotic Interactions, Nutrient Cycling and Human Affairs
数字化 TCN:协作:大型真菌收集联盟:解锁生物多样性资源以了解生物相互作用、养分循环和人类事务
  • 批准号:
    1206134
  • 财政年份:
    2012
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
Into the Heart of an Epidemic: a US-Brazil Collaboration for Integrative Studies of the Amphibian-Killing Fungus in Brazil
深入流行病的核心:美国和巴西合作对巴西的两栖类杀死真菌进行综合研究
  • 批准号:
    1159513
  • 财政年份:
    2012
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant
Digitization TCN Collaborative Research: North American Lichens and Bryophytes: Sensitive Indicators of Environmental Quality and Change
数字化 TCN 合作研究:北美地衣和苔藓植物:环境质量和变化的敏感指标
  • 批准号:
    1115030
  • 财政年份:
    2011
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Standard Grant

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合作研究:CSBR:所有权转让:活畜:丛枝菌根真菌国际菌种保藏中心 (INVAM)
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  • 批准号:
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Collaborative Research: CSBR: Ownership Transfer: Living Stocks: International Culture Collection of Arbuscular Mycorrhizal Fungi (INVAM)
合作研究:CSBR:所有权转让:活畜:丛枝菌根真菌国际菌种保藏中心 (INVAM)
  • 批准号:
    2027458
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CSBR: Transfer of Ownership: Proposal to salvage, integrate and house the LFCC herbarium at George Mason University.
CSBR:所有权转让:关于挽救、整合和容纳乔治梅森大学 LFCC 植物标本室的提案。
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  • 批准号:
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CSBR: Living Stocks -- Transfer of Ownership of Two Large Legacy Collections to the Arabidopsis Biological Resource Center
CSBR:活体种群——将两个大型遗产收藏品的所有权转让给拟南芥生物资源中心
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    1928379
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    1756169
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    $ 10.76万
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  • 批准号:
    1745618
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CSBR-Ownership Transfer: Curate, Preserve and Transfer Agriculturally and Environmentally Significant Microorganisms to established laboratories equipped for preservation and distr
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    $ 10.76万
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CSBR: Ownership Transfer: Securing the future and accessibility of the Carl W. and Marian E. Rettenmeyer army ant guest collection
CSBR:所有权转让:确保 Carl W. 和 Marian E. Rettenmeyer 军蚁客藏的未来和可访问性
  • 批准号:
    1561640
  • 财政年份:
    2016
  • 资助金额:
    $ 10.76万
  • 项目类别:
    Continuing Grant
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