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DISSERTATION RESEARCH: Mechanisms of host preference in the ectomycorrhizal symbiosis

论文研究：外生菌根共生中宿主偏好的机制

基本信息

批准号：
1600724
负责人：
Kabir Peay
金额：
$ 2.05万
依托单位：
Stanford University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-06-01 至 2019-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1600724&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Mechanisms host preference

项目摘要

Although mutually beneficial interactions are important in many natural systems, it remains unclear why some involve highly specialized partners, while others do not. This project investigates how different degrees of specificity have evolved and function in a particular type of symbiosis - a mutualistic relationship between a fungus and many temperate forest plants. This research will combine a fungus with multiple, distantly related host plants to investigate how the fungus alters its symbiotic behavior when interacting with different species of plants. This experiment will generate insight into symbiotic plant-microbe communication in this important mutualism, and will allow for comparison to similar systems in pathogens. Outreach to science enrichment programs for urban youth in San Francisco will also occur.This study will use RNA sequencing to determine the genetic basis of generalist host preference in the ectomycorrhizal fungus Thelephora terrestris. Six plant species from four families will be established in microcosms and inoculated with spores from Thelephora terrestris. At symbiotic maturity, microcosms will be labeled with carbon-13 and nitrogen-15 and plant, fungal, and symbiotic tissues will be harvested for isotope analysis and RNA sequencing. Transcriptional data will be combined with stable isotope enrichment measurements of plant and fungal resource allocation and compared across different plant-fungus combinations. Measuring gene expression in symbiotic tissues while simultaneously tracking resource allocation will reveal which genes are associated with improved symbiotic performance, providing a powerful link between gene transcription, physiological function, and the stability of mutualistic interactions.

尽管互利的相互作用在许多自然系统中很重要，但目前尚不清楚为什么有些系统涉及高度专业化的伙伴，而另一些系统则没有。这个项目研究了不同程度的特异性是如何在一种特殊类型的共生关系中进化和发挥作用的——一种真菌和许多温带森林植物之间的互惠关系。本研究将真菌与多个远亲寄主植物结合，研究真菌在与不同种类植物相互作用时如何改变其共生行为。这一实验将对植物-微生物在这一重要互惠关系中的共生交流产生深刻的见解，并将允许与病原体中的类似系统进行比较。此外，还将开展针对旧金山城市青年的科学充实项目。本研究将利用RNA测序来确定外生菌根真菌地象（theselephora terrestris）的通才寄主偏好的遗传基础。来自四个科的六种植物将在微型环境中建立，并接种地象菌的孢子。在共生成熟时，微生物将被标记为碳-13和氮-15，植物、真菌和共生组织将被收集用于同位素分析和RNA测序。转录数据将与植物和真菌资源分配的稳定同位素富集测量相结合，并在不同的植物-真菌组合中进行比较。测量共生组织中的基因表达，同时跟踪资源分配，将揭示哪些基因与改善共生性能有关，从而在基因转录、生理功能和互惠相互作用的稳定性之间提供强有力的联系。