Collaborative Research: Testing the drivers and scale-dependency of plant-fungal-bacterial community co-assembly across the Arctic

合作研究：测试北极地区植物-真菌-细菌群落共同组装的驱动因素和规模依赖性

• 批准号: 1603501
• 负责人: Donald Taylor
• 金额: $ 22.82万
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1603501&HistoricalAwards=false
• 关键词: Collaborative; Research; Testing; drivers; scale

Proposed Award AbstractArctic vegetation is rapidly changing in response to broad-scale Arctic warming. The investigators of this proposal argue that only by exploring how plants, soil microbes, and soil fungal communities interact and evolve together can we truly understand how vegetation may change in the future. Using over 3500 soil samples that have been collected across the arctic, along with environmental and plant data at the same sites, the investigators will employ new genetic and statistical techniques to understand the complex plant-microbe-fungal relations. While the work tackles a number of basic ecological questions, it will also provide insights into the controls determining how vegetation may change in the near future.The University of New Mexico is a certified Hispanic Serving Institution and of the 1600 undergraduate Biology majors, over 30% are Hispanic. Taylor and Takacs-Vesbach will provide project related research experience to these and other under-represented students. Taylor and Takacs-Vesbach are currently mentor students through three STEM programs based in the Biology department. A letter of support is provided by the Director of the UNM Minority Access to Research Careers (MARC) Program, which commits him to helping the program recruit MARC Program Scholars for training in microbiome methods. The proposal includes for the training of a female post-doctoral scientist at the University of Alaska, Fairbanks.The investigators pose a series of hypotheses that focus on the ecological drivers of community assembly (dispersal, environmental filtering, competition, facilitation, niche partitioning, neutral processes) across five Arctic biomes. They will use over 3500 soil samples that have been collected along two latitudinal transects, each of which includes all five Arctic bioclimatic subzones and
spans 1700 kilometers north to south. Using their extensive sampling and next-generation-sequencing-derived data on community composition of soil fungi and bacteria that are already in hand, combined with data on plant communities at the same sites (which will be augmented with limited sequencing of plant phylogenetic markers), the investigators seek to examine the fundamental patterns of community assembly in each of the three Kingdoms at hierarchical spatial and ecological scales.

建议奖项摘要北极植被正在迅速变化，以应对大范围的北极变暖。该提案的研究人员认为，只有探索植物、土壤微生物和土壤真菌群落如何共同作用和进化，我们才能真正了解未来植被可能发生的变化。利用在北极各地收集的3500多个土壤样本，以及同一地点的环境和植物数据，研究人员将使用新的遗传和统计技术来了解复杂的植物-微生物-真菌关系。虽然这项工作解决了一些基本的生态学问题，但它也将为决定植被在不久的将来如何变化的控制提供见解。新墨西哥大学是一所经过认证的拉美裔服务机构，在1600名本科生生物学专业中，超过30%是西班牙裔。Taylor和Takacs-Vesbach将为这些和其他代表不足的学生提供与项目相关的研究经验。Taylor和Takacs-Vesbach目前在生物系通过三个STEM项目指导学生。新墨西哥大学少数民族研究职业机会(MARC)计划主任提供了一封支持信，承诺帮助该计划招募MARC计划学者进行微生物组方法培训。该提案包括在阿拉斯加大学费尔班克斯分校培训一名女性博士后科学家。研究人员提出了一系列假设，重点关注北极五个生物群群落聚集的生态驱动因素(扩散、环境过滤、竞争、促进、生态位划分、中性过程)。他们将使用沿两个纬度横断面收集的3500多个土壤样本，每个横断面包括北极所有五个生物气候亚区，横跨南北1700公里。利用他们广泛的采样和已有的关于土壤真菌和细菌群落组成的下一代测序数据，结合相同地点的植物群落数据(将通过有限的植物系统发育标记测序来扩大数据)，研究人员试图在分层的空间和生态尺度上检查三个王国中每一个国家的群落聚集的基本模式。