Optically Tweezered Localized Plasmonic Structured Illumination Microscopy (OT-LPSIM) for Ultrafast Super Resolution Bio-imaging

用于超快超分辨率生物成像的光镊局部等离子体结构照明显微镜 (OT-LPSIM)

• 批准号: 1604216
• 负责人: Zhaowei Liu
• 金额: $ 42.52万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-15 至 2020-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1604216&HistoricalAwards=false
• 关键词: Optically; Tweezered; Localized; Plasmonic; Structured

Liu 1604216ABSTRACTThe research will develop a unique optical instrument for super resolution imaging at ultra-fast speed. The instrument will be the first optical imaging tool that has unprecedented resolution in both space (~50nm scale) and time (~20ms scale) simultaneously. It will have a profound impact to the fields of physical science, biomedical imaging and to fields where both high resolution and high speed optical imaging are required.An innovative combination of plasmonics, structured illumination microscopy, and optical tweezers, will allow a host of fundamental studies on the nanoscale interactions and real-time dynamics of living cells that are impossible to conduct by any other presently available instrument. Localized plasmonic structured illumination (LPSIM) is a structured illumination microscopy (SIM) technique and the plasmonic antenna array provides deep sub-wavelength illumination patterns, leading to much higher resolution in a final reconstructed image..

刘1604216摘要该研究将开发一种独特的光学仪器，以超快的速度进行超分辨率成像。该仪器将是第一个同时在空间（~ 50 nm尺度）和时间（~ 20 ms尺度）上具有前所未有的分辨率的光学成像工具。它将对物理科学、生物医学成像以及需要高分辨率和高速光学成像的领域产生深远的影响。等离子体激元、结构照明显微镜和光镊的创新组合将允许对活细胞的纳米级相互作用和实时动力学进行大量的基础研究，这是目前任何其他可用仪器都无法进行的。局部等离子体结构化照明（LPSIM）是一种结构化照明显微术（SIM）技术，并且等离子体天线阵列提供深亚波长照明图案，从而在最终重建图像中产生高得多的分辨率。