Optically Tweezered Localized Plasmonic Structured Illumination Microscopy (OT-LPSIM) for Ultrafast Super Resolution Bio-imaging

刘1604216这项研究将开发一种独特的光学仪器，用于超快速度的超分辨率成像。该仪器将是第一个同时在空间(~50 nm尺度)和时间(~20ms尺度)上具有前所未有的分辨率的光学成像工具。它将对物理科学、生物医学成像以及需要高分辨率和高速光学成像的领域产生深远的影响。等离子体、结构照明显微镜和光学镊子的创新组合将使人们能够对活细胞的纳米尺度相互作用和实时动力学进行一系列基础研究，这是现有任何现有仪器都无法进行的。局域等离子体结构照明(LPSIM)是一种结构照明显微镜(SIM)技术，等离子体天线阵列提供深度亚波长照明图案，导致最终重建图像的分辨率更高。

Liu 1604216ABSTRACTThe research will develop a unique optical instrument for super resolution imaging at ultra-fast speed. The instrument will be the first optical imaging tool that has unprecedented resolution in both space (~50nm scale) and time (~20ms scale) simultaneously. It will have a profound impact to the fields of physical science, biomedical imaging and to fields where both high resolution and high speed optical imaging are required.An innovative combination of plasmonics, structured illumination microscopy, and optical tweezers, will allow a host of fundamental studies on the nanoscale interactions and real-time dynamics of living cells that are impossible to conduct by any other presently available instrument. Localized plasmonic structured illumination (LPSIM) is a structured illumination microscopy (SIM) technique and the plasmonic antenna array provides deep sub-wavelength illumination patterns, leading to much higher resolution in a final reconstructed image..