Understanding Rotational Dynamics of Cargo in Intracellular Transport with Plasmonic Nanoparticle Probes

用等离子体纳米粒子探针了解细胞内运输中货物的旋转动力学

• 批准号: 1604612
• 负责人: Ning Fang
• 金额: $ 30万
• 依托单位: Georgia State University Research Foundation, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1604612&HistoricalAwards=false
• 关键词: Understanding; Rotational; Dynamics; Cargo; Intracellular

Fang1004612A cell can be conceived as a factory containing a network of nanomachines. Many of these nanomachines transduce chemical energy into mechanical work in a cyclic manner. The malfunction of any of these nanomachines can lead to severe disease. There is an urgent need from the health and medicine sector for new innovative techniques to investigate the composition, dynamics, and working mechanism of the nanomachines in live cells. Translational motion can be readily revealed by a variety of conventional single particle tracking (SPT) methods. However, rotational motion in live cells is much more difficult to resolve and largely unknown due to challenging technical limitations. The outcomes of the proposed research will lay the groundwork for development of treatments for conditions caused by the malfunction of the cellular nanomachinery, ranging from certain kinds of blindness and kidney disease to neurodegenerative disorders and parasitic diseases. The research will be integrated into the curriculum and will include undergraduate optical imaging laboratory experiments. The PI proposes to develop a new imaging system based on single particle orientation and rotational tracking (SPORT), a technology invented by the PI. This system will provide 5 dimensional information on particles (xyz location plus two angular orientation) to better understand intracellular cargo transport. The PI will develop optical microscopy methods to track the rotation of single gold nanorods attached to intracellular cargo, such as vesicles and motor proteins, which when combined with x,y, and z translations will offer more complete insight into their dynamics in live cells.

Fang1004612A细胞可以被认为是一个包含纳米机器网络的工厂。这些纳米机器中的许多以循环方式将化学能转化为机械功。任何这些纳米机器的故障都可能导致严重的疾病。卫生和医学部门迫切需要新的创新技术来研究活细胞中纳米机器的组成、动力学和工作机制。平移运动可以很容易地揭示了各种传统的单粒子跟踪（SPT）方法。然而，活细胞中的旋转运动更难以解决，并且由于具有挑战性的技术限制而在很大程度上未知。拟议研究的结果将为开发由细胞纳米机器人故障引起的疾病的治疗方法奠定基础，这些疾病包括某些类型的失明和肾脏疾病，以及神经退行性疾病和寄生虫病。 这项研究将被纳入课程，并将包括本科光学成像实验室实验。PI提议开发一种基于PI发明的单粒子定向和旋转跟踪（SPORT）技术的新成像系统。该系统将提供关于颗粒的5维信息（xyz位置加上两个角取向）以更好地理解细胞内货物运输。PI将开发光学显微镜方法来跟踪连接到细胞内货物（如囊泡和马达蛋白）的单个金纳米棒的旋转，当与x，y和z平移结合时，将更全面地了解它们在活细胞中的动态。