Visualization of mechanical stress in live cells

活细胞机械应力的可视化

• 批准号: 1604562
• 负责人: Adam Wax
• 金额: $ 60万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1604562&HistoricalAwards=false
• 关键词: Visualization; mechanical; stress; live; cells

ABSTRACTWax, Adam1604562This proposal will develop novel optical methods for visualizing mechanical stresses in live cells. They will study the mechanics at the subcellular level to understand transduction of mechanical stresses and the roles of key proteins in both focal adhesion and the cell cortex.The key technical advance this project will pursue is to incorporate FRET (Forster resonance energy transfer)-based tension sensors into the current quantitative phase imaging platform. Tension sensors were developed by incorporating two fluorophores into load-bearing proteins. Traditionally dynamic loading has required indirect measures and complex devices. The proposed platform will be able to directly visualize cell stresses and to do so without mechanically displacing the cell. Further technical advances will develop FRET sensors for a load-bearing protein in the cell cortex to enable simple, reproducible loading of cells while preserving imaging capabilities.

这个提议将发展一种新的光学方法来观察活细胞中的机械应力。他们将在亚细胞水平上研究力学，以了解机械应力的传导以及关键蛋白在粘着斑和细胞皮层中的作用。该项目将追求的关键技术进步是将基于FRET（Forster共振能量转移）的张力传感器纳入当前的定量相位成像平台。张力传感器是通过将两个荧光团结合到承重蛋白质中而开发的。传统的动态加载需要间接测量和复杂的装置。所提出的平台将能够直接可视化细胞应力，并且在不机械移位细胞的情况下这样做。进一步的技术进步将开发FRET传感器，用于细胞皮层中的承重蛋白，以实现简单，可重复的细胞加载，同时保持成像能力。