Increased Depth Penetration in Coherence Imaging Using Multiply Scattered Light

1133222waxpi提出发展ms/LCI（多次散射/低相干干涉测量），通过多次散射光成像厚组织。提出的具体任务是：1。仪器开发：该系统将进行修改，使光学扫描和多光谱干涉成像成为可能。光学扫描将提高获取二维图像的速度，而包含多个照明波长将允许恢复重要的生化信息，如血红蛋白浓度和氧合。2. 理论建模：为了更好地理解成像对比的机制，PI将采取双管齐下的努力来模拟光的传播。提出了一种基于随机游走形式的分析形式，该形式将用于评估这种成像模式。为了验证这个模型，他们还将使用蒙特卡罗模型并与实验结果进行比较。3. 显示血红蛋白在厚组织中的分布。为了证明该方法的实用性，他们将对血红蛋白在厚（~1厘米）组织中的分布进行成像。样本将包括用于验证该方法的组织幻影，以及离体动物组织，如乳房成像中遇到的肌肉和脂肪，以及用于OCT眼科成像的视网膜样本，其中渗透到脉络膜仍然是一个挑战。所提出的研究将提出一种新的成像方式，具有独特的潜力，以扩大成像深度。此外，新的多光谱干涉测量仪器将推进光学工程，而提出的随机游走模型将促进对光在组织中的传播的理解。

1133222WaxThe PI proposes to develop ms/LCI (multiple scattering/low coherence interferometry) to image through thick tissues with multiply scattered light. The specific tasks proposed are: 1. Instrument development:The system will be modified to enable optical scanning and multispectral interferometric imaging. Optical scanning will improve the speed of acquiring 2D images while inclusion of multiple illumination wavelengths will allow recovery of important biochemical information such as hemoglobin concentration and oxygenation. 2. Theoretical modeling:To better understand the mechanisms of imaging contrast in this approach the PI will take a dual pronged effort to model the light propagation. An analytical form is presented which is based on a random walk formalism that will be used to evaluate this imaging modality. To validate this model, they will also use Monte Carlo modeling as well as comparing to experimental results. 3. Demonstrate imaging of hemoglobin distribution in thick tissues.To demonstrate the utility of the method, they will image hemoglobin distribution through thick (~1 cm) tissues. Samples will include tissue phantoms, designed to validate the method, and ex vivo animal tissues, such as muscle and fat as would be encountered in breast imaging and retinal samples as would be used in OCT ophthalmic imaging, where penetration to the choroid has remained a challenge. The proposed research will advance a novel imaging modality with unique potential to extend imaging depth. In addition, new multispectral interferometry instrumentation will advance optical engineering while the proposed random walk model will advance understanding of light propagation in tissues.