Molecular imaging of live cells using hyperspectral darkfield microscopy

使用高光谱暗场显微镜对活细胞进行分子成像

• 批准号: 0651622
• 负责人: Adam Wax
• 金额: $ 60万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0651622&HistoricalAwards=false
• 关键词: Molecular; imaging; live; cells; using

CBET-0651622 Wax The goal of this research is to develop a novel optical system for molecular imaging of live cells based on hyperspectral darkfield microscopy. In particular, the proposed approach is based on detecting surface plasmon resonance (SPR) of functionally labeled nanoparticles. The PI proposes to use particles tuned to specific spectral windows. Four species of nanoparticles will be synthesized. This will enable detection of a number of molecular targets simultaneously. Current nanoparticle-based imaging techniques detect the overall increase in scattered light due to nanoparticle binding, whereas the approach proposed here is based on the measurement of the spectral dependence of the scattering. These spectral properties depend on the local refractive index environment of a nanoparticle. Thus, spectral analysis of light scattered by the nanoparticles can be used not only to detect binding but also to sense changes in local environment.

本研究的目的是开发一种基于高光谱暗场显微镜的新型活细胞分子成像光学系统。特别是，所提出的方法是基于检测表面等离子体共振（SPR）的功能标记纳米颗粒。PI建议使用调谐到特定光谱窗口的粒子。四种纳米粒子将被合成。这将使同时检测多个分子靶标成为可能。目前基于纳米粒子的成像技术检测到由于纳米粒子结合而导致的散射光的总体增加，而本文提出的方法是基于散射光谱依赖性的测量。这些光谱特性取决于纳米粒子的局部折射率环境。因此，纳米粒子散射光的光谱分析不仅可以用于检测结合，还可以用于感知局部环境的变化。