Label-free, chemiresistive, paper microfluidic nanobiosensor array for multiplexed detection

PI：Tsutsui，Hideaki Proposal No：1606181本项目建议开发一种纸基分子传感平台。它的主要优点是成本低，使用方便。这项拟议的工作有望扩大纸基传感器在医疗、农业和环境测试与诊断等领域的能力和应用。纸基微流控设备最近已成为满足社会许多部门诊断需求的有前途的传感平台。纸基微流控传感器的优点包括快速检测、易于制造和使用、对仪器的要求最低、重量轻、便于携带和低成本。然而，由于缺乏可在纸衬底上轻松实施的高灵敏检测方法，它在研究实验室以外的广泛使用受到了阻碍。该项目的总体目标是开发一种无标记、高灵敏度、耐化学变化的纳米传感器平台。这一目标将通过一系列研究任务来实现：1)在纸上开发超灵敏的碳纳米管化学电阻生物传感器；2)开发有效的流体处理技术；以及3)集成和演示作为模型抗原的心脏生物标志物的无标记、多路检测。智能方面的优点包括由半导体单壁碳纳米管制成的高度敏感的传感元件，以及微流控传输的技术创新，这些共同使所提出的纳米传感器成为可能。该研究计划的更广泛影响是扩大了纸基微流控生物传感器的能力和应用。特别是，所提出的无标记抗化学性生物传感器阵列有望具有高灵敏度、简便性和经济性。它还有望容易地应用于多种复杂流体基质中分析物的多重检测，如食品、水、植物和体液。在开展研究活动的同时，该项目将开发和整合若干外联和教育活动，包括编制关于纸基微流控生物传感器的新课程，对本科生和研究生，特别是未被充分代表的少数群体和妇女进行研究培训，并开展外联努力，使加州内陆帝国地区的K-12教师和学生参与进来。

PI: Tsutsui, HideakiProposal No: 1606181 This project proposes to develop a paper-based molecular sensing platform. The primary advantages are its low cost and ease of use. The proposed work is expected to broadly expand the capability and applications of paper-based sensors for a variety of fields, such as medical, agricultural, and environmental testing and diagnosis.Paper-based microfluidic devices have recently emerged as promising sensing platforms for serving the diagnostic needs in many sectors of society. Advantages of paper-based microfluidic sensors include fast detection, ease of fabrication and use, minimal instrument requirements, lightweight, portability, and low cost. However, its widespread use beyond research laboratories has been hampered because of a lack of highly sensitive detection methods that can be easily implemented on a paper substrate. The overall goal of this project is to develop a label-free, highly sensitive, chemiresistive nanosensor platform. This goal will be achieved through a series of research tasks: 1) development of an ultrasensitive carbon nanotube chemiresistive biosensor on paper; 2) development of an effective fluid handling technology; and 3) integration and demonstration of label-free, multiplexed detection of cardiac biomarkers as model antigens. Intellectual merits include a highly sensitive sensing element made of semiconducting single-walled carbon nanotubes and technological innovations of microfluidic transport, which collectively enable the proposed nanosensor. Broader impact of the research program is expansion of the capability and applications of the paper-based microfluidic-facilitated biosensors. In particular, the proposed label-free chemiresistive biosensor array is expected to be highly sensitive, facile, and economical. It is also expected to be readily applicable to the multiplexed detection of analytes in a variety of complex fluid matrices such as food, water, plants, and body fluids. In conjunction with research activities, this project will develop and integrate several outreach and educational activities, including new curricula development on paper-based microfluidic biosensors, research training of both undergraduate and graduate students, particularly underrepresented minorities and women, and outreach efforts to engage K-12 teachers and students of the Inland Empire region of California.