Label-free, chemiresistive, paper microfluidic nanobiosensor array for multiplexed detection

PI：Tsutsui，HideakiProposal No：1606181本项目旨在开发一种基于纸张的分子传感平台。其主要优点是成本低和易于使用。 这项工作有望广泛扩展纸基传感器的能力和应用，用于医疗、农业和环境测试和诊断等领域。纸基微流控器件最近成为一种有前途的传感平台，可满足社会许多部门的诊断需求。纸基微流体传感器的优点包括快速检测、易于制造和使用、最小的仪器要求、重量轻、便携性和低成本。然而，由于缺乏可以在纸质基底上容易实施的高度灵敏的检测方法，其在研究实验室之外的广泛使用受到阻碍。该项目的总体目标是开发一种无标记，高灵敏度，化学电阻纳米传感器平台。这一目标将通过一系列研究任务来实现：1）开发纸上超灵敏的碳纳米管化学电阻生物传感器; 2）开发有效的流体处理技术; 3）集成和演示作为模型抗原的心脏生物标志物的无标记，多重检测。智能优点包括由半导体单壁碳纳米管制成的高灵敏度传感元件和微流体运输的技术创新，这些都使拟议的纳米传感器成为可能。该研究计划的更广泛的影响是扩展基于纸的微流体促进生物传感器的能力和应用。特别地，所提出的无标记化学电阻生物传感器阵列预期是高度灵敏的、容易的和经济的。它也有望容易地适用于多种复杂的流体基质，如食品，水，植物和体液中的分析物的多重检测。在研究活动的同时，该项目将开发和整合几个推广和教育活动，包括新的课程开发基于纸的微流体生物传感器，研究培训的本科生和研究生，特别是代表性不足的少数民族和妇女，以及推广工作，从事K-12教师和学生的内陆帝国地区的加州。

PI: Tsutsui, HideakiProposal No: 1606181 This project proposes to develop a paper-based molecular sensing platform. The primary advantages are its low cost and ease of use. The proposed work is expected to broadly expand the capability and applications of paper-based sensors for a variety of fields, such as medical, agricultural, and environmental testing and diagnosis.Paper-based microfluidic devices have recently emerged as promising sensing platforms for serving the diagnostic needs in many sectors of society. Advantages of paper-based microfluidic sensors include fast detection, ease of fabrication and use, minimal instrument requirements, lightweight, portability, and low cost. However, its widespread use beyond research laboratories has been hampered because of a lack of highly sensitive detection methods that can be easily implemented on a paper substrate. The overall goal of this project is to develop a label-free, highly sensitive, chemiresistive nanosensor platform. This goal will be achieved through a series of research tasks: 1) development of an ultrasensitive carbon nanotube chemiresistive biosensor on paper; 2) development of an effective fluid handling technology; and 3) integration and demonstration of label-free, multiplexed detection of cardiac biomarkers as model antigens. Intellectual merits include a highly sensitive sensing element made of semiconducting single-walled carbon nanotubes and technological innovations of microfluidic transport, which collectively enable the proposed nanosensor. Broader impact of the research program is expansion of the capability and applications of the paper-based microfluidic-facilitated biosensors. In particular, the proposed label-free chemiresistive biosensor array is expected to be highly sensitive, facile, and economical. It is also expected to be readily applicable to the multiplexed detection of analytes in a variety of complex fluid matrices such as food, water, plants, and body fluids. In conjunction with research activities, this project will develop and integrate several outreach and educational activities, including new curricula development on paper-based microfluidic biosensors, research training of both undergraduate and graduate students, particularly underrepresented minorities and women, and outreach efforts to engage K-12 teachers and students of the Inland Empire region of California.