Label-free, chemiresistive, paper microfluidic nanobiosensor array for multiplexed detection

PI: Tsutsui， HideakiProposal No: 1606181本项目提出开发一种基于纸张的分子传感平台。其主要优点是成本低，使用方便。拟议的工作预计将广泛扩展基于纸张的传感器在各种领域的能力和应用，例如医疗、农业和环境测试和诊断。基于纸张的微流控装置最近成为服务于社会许多部门诊断需求的有前途的传感平台。基于纸张的微流体传感器的优点包括快速检测，易于制造和使用，最小的仪器要求，重量轻，便携性和低成本。然而，由于缺乏可以在纸基板上轻松实现的高灵敏度检测方法，它在研究实验室之外的广泛应用受到了阻碍。该项目的总体目标是开发一种无标签、高灵敏度、耐化学性的纳米传感器平台。这一目标将通过一系列的研究任务来实现：1)开发一种超灵敏的纸上碳纳米管化学电阻生物传感器；2)开发有效的流体处理技术；3)整合和演示无标记、多路检测心脏生物标志物作为模型抗原。知识上的优点包括由半导体单壁碳纳米管制成的高灵敏度传感元件和微流体传输的技术创新，这些共同使所提出的纳米传感器成为可能。该研究计划的更广泛影响是扩展了基于纸的微流体促进生物传感器的能力和应用。特别是，所提出的无标签化学阻性生物传感器阵列有望具有高灵敏度、轻便性和经济性。它也有望很容易地适用于多种复杂流体基质（如食物、水、植物和体液）中分析物的多重检测。与研究活动相结合，该项目将开发和整合几项外展和教育活动，包括纸基微流体生物传感器的新课程开发，本科生和研究生的研究培训，特别是代表性不足的少数民族和女性，以及吸引加州内陆帝国地区K-12教师和学生的外展努力。

PI: Tsutsui, HideakiProposal No: 1606181 This project proposes to develop a paper-based molecular sensing platform. The primary advantages are its low cost and ease of use. The proposed work is expected to broadly expand the capability and applications of paper-based sensors for a variety of fields, such as medical, agricultural, and environmental testing and diagnosis.Paper-based microfluidic devices have recently emerged as promising sensing platforms for serving the diagnostic needs in many sectors of society. Advantages of paper-based microfluidic sensors include fast detection, ease of fabrication and use, minimal instrument requirements, lightweight, portability, and low cost. However, its widespread use beyond research laboratories has been hampered because of a lack of highly sensitive detection methods that can be easily implemented on a paper substrate. The overall goal of this project is to develop a label-free, highly sensitive, chemiresistive nanosensor platform. This goal will be achieved through a series of research tasks: 1) development of an ultrasensitive carbon nanotube chemiresistive biosensor on paper; 2) development of an effective fluid handling technology; and 3) integration and demonstration of label-free, multiplexed detection of cardiac biomarkers as model antigens. Intellectual merits include a highly sensitive sensing element made of semiconducting single-walled carbon nanotubes and technological innovations of microfluidic transport, which collectively enable the proposed nanosensor. Broader impact of the research program is expansion of the capability and applications of the paper-based microfluidic-facilitated biosensors. In particular, the proposed label-free chemiresistive biosensor array is expected to be highly sensitive, facile, and economical. It is also expected to be readily applicable to the multiplexed detection of analytes in a variety of complex fluid matrices such as food, water, plants, and body fluids. In conjunction with research activities, this project will develop and integrate several outreach and educational activities, including new curricula development on paper-based microfluidic biosensors, research training of both undergraduate and graduate students, particularly underrepresented minorities and women, and outreach efforts to engage K-12 teachers and students of the Inland Empire region of California.