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CAREER: Printable and Injectable Chromatic Nanosensor for One-Step, Naked-Eye Detection

职业：可打印和可注射的彩色纳米传感器，用于一步式肉眼检测

基本信息

批准号：
1654010
负责人：
Hideaki Tsutsui
金额：
$ 50.03万
依托单位：
University of California-Riverside
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-02-01 至 2023-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1654010&HistoricalAwards=false
关键词：
CAREER Printable Injectable Chromatic Nanosensor

项目摘要

PI: Tsutsui, Hideaki Proposal No: 1654010 The career objective is, through integration of research and education, to innovate low-cost, point-of-use diagnostic technologies and train the next generation of globally engaged STEM leaders. Specifically, the goal is to significantly expand functionality of polydiacetylene-based biosensors for providing people in resource-limited regions with highly effective agricultural and medical disease diagnostic tools.This project aims to use polydiacetylenes(PDA) polymer to develop low-cost colorimetric sensors that can be directly injected into plants for detecting various pathogenic bacteria. If successful the novel sensors can have a broad array of applications. The main hypothesis of the proposed research is that, by optimizing the capture probe configuration and the PDA composition, it will be possible to significantly improve sensitivity and detection limits of the PDA biosensors. An optimal combination of the probe length, conformation, and linker length will be identified. Second, optimal combination of the diacetylene structure, phospholipid additives, and vesicle size will be determined. These activities will shed light on the roles of each controllable parameter in experimentally observed sensor performances. Subsequently, successful capture probe and PDA designs will be used for developing printable sensors on paper substrates and injectable vesicle sensors in suspension, both targeting a bacterial plant pathogen, Pantoea stewartii. Collectively, this project is expected to produce new knowledge in design of PDA biosensor parameters and their role in signal generation and transduction. End application will include disseminating low cost sensor technology in East Africa through collaborators. In addition, a new undergraduate/graduate course on low cost diagnostic technology will be introduced and outreach to K-12 teachers and students is also planned. Online educational and documentary videos will be created to teach national and global audiences.

主要研究者：Tsutsui，Hideaki提案编号：1654010职业目标是，通过研究和教育的整合，创新低成本，使用点诊断技术和培养下一代全球参与干领导者。具体而言，本项目的目标是大幅度扩展基于聚二乙炔的生物传感器的功能，为资源有限地区的人们提供高效的农业和医疗疾病诊断工具。本项目的目标是利用聚二乙炔（PDA）聚合物开发可直接注入植物中检测各种病原菌的低成本比色传感器。如果成功，这种新型传感器可以有广泛的应用。提出的研究的主要假设是，通过优化捕获探针的配置和PDA的组成，它将有可能显着提高灵敏度和检测限的PDA生物传感器。将鉴定探针长度、构象和接头长度的最佳组合。其次，将确定二乙炔结构、磷脂添加剂和囊泡大小的最佳组合。这些活动将揭示实验观察到的传感器性能的每个可控参数的作用。随后，成功的捕获探针和PDA设计将用于开发纸基板上的可打印传感器和悬浮液中的可注射囊泡传感器，这两种传感器都针对细菌植物病原体Pantoea stewartii。总的来说，这个项目预计将产生新的知识，在设计的PDA生物传感器参数和它们的作用，信号的产生和转导。最终应用将包括通过合作者在东非传播低成本传感器技术。此外，还将开设一门关于低成本诊断技术的新的本科生/研究生课程，并计划向K-12教师和学生推广。将制作在线教育和纪录片视频，向国家和全球观众传授知识。