CAREER: Printable and Injectable Chromatic Nanosensor for One-Step, Naked-Eye Detection

PI：Tsutsui，Hideaki Proposal No：1654010职业目标是，通过研究和教育的整合，创新低成本、使用点诊断技术，并培养下一代全球参与的STEM领导者。具体地说，该项目的目标是显著扩展基于聚二乙炔的生物传感器的功能，为资源有限地区的人们提供高效的农业和医疗疾病诊断工具。该项目旨在利用聚二乙炔(PDA)聚合物开发低成本的比色传感器，可以直接注入植物中检测各种病原菌。如果成功，这种新型传感器可以有广泛的应用。这项研究的主要假设是，通过优化捕获探针的配置和PDA的组成，将有可能显著提高PDA生物传感器的灵敏度和检测限。将确定探针长度、构象和连接物长度的最佳组合。其次，将确定二乙炔结构、磷脂添加剂和囊泡大小的最佳组合。这些活动将阐明每个可控参数在实验观察到的传感器性能中的作用。随后，成功的捕获探针和PDA设计将用于开发纸基上的可打印传感器和悬浮液中的可注射囊泡传感器，这两种传感器都针对一种细菌植物病原体Pantoea stewartii。总而言之，该项目有望在PDA生物传感器参数的设计以及它们在信号产生和转导中的作用方面产生新的知识。最终应用将包括通过合作者在东非传播低成本传感器技术。此外，还将推出一门关于低成本诊断技术的新的本科生/研究生课程，并计划向K-12教师和学生进行宣传。将制作在线教育和纪录片，向全国和全球观众传授知识。

PI: Tsutsui, Hideaki Proposal No: 1654010 The career objective is, through integration of research and education, to innovate low-cost, point-of-use diagnostic technologies and train the next generation of globally engaged STEM leaders. Specifically, the goal is to significantly expand functionality of polydiacetylene-based biosensors for providing people in resource-limited regions with highly effective agricultural and medical disease diagnostic tools.This project aims to use polydiacetylenes(PDA) polymer to develop low-cost colorimetric sensors that can be directly injected into plants for detecting various pathogenic bacteria. If successful the novel sensors can have a broad array of applications. The main hypothesis of the proposed research is that, by optimizing the capture probe configuration and the PDA composition, it will be possible to significantly improve sensitivity and detection limits of the PDA biosensors. An optimal combination of the probe length, conformation, and linker length will be identified. Second, optimal combination of the diacetylene structure, phospholipid additives, and vesicle size will be determined. These activities will shed light on the roles of each controllable parameter in experimentally observed sensor performances. Subsequently, successful capture probe and PDA designs will be used for developing printable sensors on paper substrates and injectable vesicle sensors in suspension, both targeting a bacterial plant pathogen, Pantoea stewartii. Collectively, this project is expected to produce new knowledge in design of PDA biosensor parameters and their role in signal generation and transduction. End application will include disseminating low cost sensor technology in East Africa through collaborators. In addition, a new undergraduate/graduate course on low cost diagnostic technology will be introduced and outreach to K-12 teachers and students is also planned. Online educational and documentary videos will be created to teach national and global audiences.