Development of scanning optofluidic cell lasers for highly sensitive cellular and tissue analysis

开发用于高灵敏度细胞和组织分析的扫描光流控细胞激光器

• 批准号: 1607250
• 负责人: Xudong Fan
• 金额: $ 45.43万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1607250&HistoricalAwards=false
• 关键词: Development; scanning; optofluidic; cell; lasers

Title: Development of scanning optofluidic cell lasers for highly sensitive cellular and tissue analysisThe goal of the project: Develop a novel laser based scanning microscope to study cells and tissues.Non-Technical Abstract:The traditional microscope relies on fluorescence to study cells and tissues. However, any small change in cellular activities and tissue structures may not be picked up by the microscope. The proposed project is aimed to develop a laser-based microscope in which laser emission instead of fluorescence is used for detection. Laser emission is much more sensitive than fluorescence. Therefore, small changes in cellular/tissue activities will cause large signals in laser emission, which allows us to study cell and tissues at a more detailed level.The proposed research will provide a new, powerful, and sensitive platform technology complementary to conventional fluorescence based methods for cell/tissue analysis. The detection principle developed through the project can broadly be applicable to any types of laser cavities and bio-species. The proposed research will further provide in-depth understanding of how light interacts with living organisms and biological materials, which will be significant for the development of novel photonic devices. The students in the project will receive interdisciplinary training in optofluidics, photonics, nano/microfabrication, biochemistry, cell biology, and biosensing. The outreach program will educate the general public and train students/teachers from local high schools and non-research-intensive colleges.Technical Abstract:The proposed project is to systematically investigate the biofunctional scanning optofluidic cell laser technology platform and apply it in highly sensitive cell/tissue analysis. The optofluidic laser is an emerging sensing technology that integrates microfluidics, optical microcavity, and gain medium in liquid environment. It employs laser emission as the sensing signal. A small modulation in the gain medium induced by a small change in the underlying biological activities can result in a significant change in the laser output due to optical feedback provided by the laser cavity. Thus, the optofluidic laser based detection is orders of magnitude more sensitive than the fluorescence counterpart. The proposed scanning optofluidic laser is based on a high-performance laser cavity and cells/tissues containing gain media. The laser output will be recorded for detailed understanding of cellular/tissue activities.The proposed research will provide a new, powerful, and sensitive platform technology complementary to conventional fluorescence based methods for cell/tissue analysis. The detection principle developed through the project can broadly be applicable to any types of laser cavities and bio-species. Furthermore, study of the optofluidic cell lasers will provide in-depth understanding of how light interacts with living organisms and biological materials, which will be significant for the development of novel photonic devices. The students in the project will receive interdisciplinary training in optofluidics, photonics, nano/microfabrication, biochemistry, cell biology, and biosensing. The outreach program will educate the general public and train students/teachers from local high schools and non-research-intensive colleges.

职务名称：本项目的目标是：开发一种新型的基于激光的扫描显微镜，用于研究细胞和组织。非技术摘要：传统的显微镜依赖于荧光来研究细胞和组织。然而，细胞活动和组织结构的任何微小变化都可能不会被显微镜发现。拟议的项目旨在开发一种基于激光的显微镜，其中激光发射而不是荧光用于检测。激光发射比荧光灵敏得多。因此，细胞/组织活动的微小变化将导致激光发射的大信号，这使我们能够在更详细的水平上研究细胞和组织。拟议的研究将提供一个新的，强大的，灵敏的平台技术，以补充传统的基于荧光的细胞/组织分析方法。通过该项目开发的检测原理可广泛适用于任何类型的激光腔和生物物种。拟议的研究将进一步深入了解光如何与生物体和生物材料相互作用，这将对新型光子器件的开发具有重要意义。该项目的学生将接受光流体学，光子学，纳米/微制造，生物化学，细胞生物学和生物传感的跨学科培训。该推广计划将教育公众，并培训当地高中和非研究密集型大学的学生/教师。技术摘要：拟议项目是系统地研究生物功能扫描光流控细胞激光技术平台，并将其应用于高灵敏度的细胞/组织分析。光流体激光器是一种集微流体、光学微腔和增益介质于一体的新型传感技术。它采用激光发射作为传感信号。由于激光腔提供的光学反馈，由潜在生物活性的小变化引起的增益介质中的小调制可以导致激光输出的显著变化。因此，基于光流体激光器的检测比荧光对应物更敏感几个数量级。所提出的扫描光流体激光器是基于高性能激光腔和含有增益介质的细胞/组织。激光输出将被记录下来，以详细了解细胞/组织的活动。拟议的研究将提供一个新的，强大的，灵敏的平台技术补充传统的荧光为基础的方法，用于细胞/组织的分析。通过该项目开发的检测原理可广泛适用于任何类型的激光腔和生物物种。此外，对光流控细胞激光器的研究将深入了解光与生物体和生物材料的相互作用，这对开发新型光子器件具有重要意义。该项目的学生将接受光流体学，光子学，纳米/微制造，生物化学，细胞生物学和生物传感的跨学科培训。该推广方案将教育公众，并培训当地高中和非研究密集型大学的学生/教师。