OP: Collaborative Research: Multimodal Molecular Spectroscopy and Imaging in Biological Tissue and Historical Artwork

在化学系化学测量和成像项目、物理系原子、分子和光学实验物理项目以及国际科学与工程办公室的支持下，Martin Fischer、Warren Warren、Khanh Kieu教授和他们的团队将开发光学显微镜技术，以创建具有新型分子和结构对比度的高分辨率图像。这些成像方法甚至可以在地表下提供详细的化学信息，并且不需要物理移除样品来创建三维地图。这些技术非常适合生物组织和历史艺术作品的成像，在这些领域，样本的移除是受限的，甚至是不可能的。从这些化学图谱中获得的信息有助于为开发诊断生物组织疾病的新方法奠定科学基础，并为修复甚至防止不可替代的艺术品的损坏或退化提供保护人员所需的信息。生物组织和历史艺术品的功能丧失，如绘画中的变色或开裂或组织中的疾病，通常在微观尺度上反映在分子组成或结构的变化上。诊断评估的一个主要挑战是，为了获得这些信息，通常需要机械取样。为了应对这一挑战，本研究试图开发一种基于非线性泵探针显微镜的多模态检测方法，以高空间分辨率在三维上无创地绘制颜料及其周围环境。具体来说，正在开发的技术获得了新的对比机制（如非线性相位对比），它提供了更高的特异性和更广泛的内源性靶点。此外，正在研究组织和艺术品中的关键降解过程，包括固有的（例如自然降解）和诱导的（例如光降解）过程。最后，该团队正在努力将使用这些技术的合适仪器传播给生物医学和保护科学界。

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, the Atomic, Molecular, and Optical Experimental Physics Program in the Division of Physics, and the Office of International Science and Engineering, Professors Martin Fischer, Warren Warren, Khanh Kieu and their groups will develop optical microscopy techniques to create high-resolution images with novel molecular and structural contrast. These imaging methods can provide detailed chemical information even below the surface and do not require the physical removal of samples to create three-dimensional maps. These techniques are ideally suited for imaging of biological tissue and historical works of art, where the removal of a sample is restricted or even impossible. Information obtained from these chemical maps can help lay the scientific foundation for the development of new methods to diagnose diseases in biological tissue and give conservators information needed to repair or even prevent damage or degradation in irreplaceable works of art.The loss of function in biological tissue and historical artworks, such as discoloration or cracking in a painting or disease in tissue, generally is reflected in changes in the molecular composition or structure on a microscopic scale. A major challenge in diagnostic evaluation is that to obtain this information, mechanical sample removal is generally required. In response to this challenge, this research seeks to develop a multimodal detection method based on nonlinear pump-probe microscopy to noninvasively map pigments and their surroundings with high spatial resolution in three dimensions. Specifically, the techniques being developed access novel contrast mechanisms (such as nonlinear phase contrast) which give much higher specificity and a wider range of endogenous targets. Furthermore, critical degradation processes are being studied in tissue and artwork, including inherent (e.g. natural degradation) and induced (e.g. photodegradation) processes. Lastly, the team is working toward dissemination of suitable instruments using these techniques to the biomedical and conservation science communities.