Bilateral BBSRC-NSF/BIO: Excitocell: A rewired eukaryotic cell model for the analysis and design of cellular morphogenesis

事实上，细胞和组织所做的每一件重要的事情都涉及细胞形态的变化：细胞分裂、细胞迁移和伤口愈合都涉及细胞形状的深刻变化。 这些形状的变化是由细胞的外层，也就是所谓的“皮层”提供动力的。 皮质包含一个纤维蛋白质网络，可以收缩、突出和滑动。 这些运动提供了细胞形状变化的基础。 控制这种纤维状网络的是一种叫做Rho的蛋白质。 Rho以不同的模式被激活，每个模式对应于不同的细胞形状变化。 因此，为了理解和控制过程，如细胞分裂，细胞迁移和伤口愈合，必须了解如何理解和控制Rho激活模式。 在这个合作项目中，来自美国和英国的研究人员使用合成细胞生物学和数学建模来测试皮层模式形成的潜在机制。 该项目将为本科生、研究生和博士后提供定量细胞生物学和数学建模之间接口的跨学科培训的研究机会。 该项目还为高中教师提供嵌入式研究机会，以及特定主题的K-12和公共宣传活动。PI先前的研究表明，细胞可以支持动态耦合Rho活性和肌动蛋白组装的持续波，数学建模和实验验证表明，皮质波传播是基于Rho自激活和肌动蛋白介导的Rho失活。 这构成了“可兴奋”系统的基础，这是一系列成熟的理论模型，几乎没有以前已知的细胞表现。 这个项目将推导和实验验证皮质兴奋性所需的最小分子机制和基本设计元素。 为了实现该项目的目标，将采用一种新的半合成平台，用于使用简单的大分子部分复制复杂的细胞行为，从而能够以不断升级的复杂性操纵分子网络，而不是通过解剖复杂的生理网络。 该项目将耦合兴奋动力学的计算建模，在整个细胞和一个新的离体模型的皮质动力学活细胞成像。 首先，可兴奋的动力学将在休息，非有丝分裂细胞（卵母细胞的青蛙和棘皮动物）使用天然调节剂和它们的突变体，然后用合成的等价物取代。 第二，将开发一种可定量的离体模型（使用青蛙卵母细胞或卵提取物和支持的脂质双层），该模型允许在简化的背景下精确控制系统组成。 第三，为了实现对皮质图案形成的按需控制，将使用动物卵母细胞中的光遗传学方法来探索天然和合成皮质图案形成的全部功能。 整个项目将采用迭代实验和计算建模，以1）解释生物数据，2）以数学模型的形式表达候选机制，3）生成预测，以及4）通过实验测试这些预测。

Virtually every important thing cells and tissues do involve changes in cell morphology: cell division, cell migration, and wound healing all involve profound changes in cell shape. These shape changes are powered by the outer layer of the cell, which is called the "cortex". The cortex contains a meshwork of fibrous proteins that can contract, protrude, and slide. These movements provide the basis of cell shape changes. The control of this fibrous network is the domain of a protein called Rho. Rho is somehow activated in distinct patterns, each of which corresponds to a different cell shape change. Thus, in order to understand and control processes such as cell division, cell migration and wound healing, it is essential to learn how to understand and control patterns of Rho activation. In this collaborative project, investigators from the US and the UK use synthetic cell biology and mathematical modeling to test mechanisms underlying cortical pattern formation. This project will provide research opportunities for interdisciplinary training at the interface between quantitative cell biology and mathematical modeling for undergraduates, graduate students, and postdocs. The project also offers embedded research opportunities for high-school teachers as well as topic-specific K-12 and public outreach activities. Previous studies by the PIs demonstrated that cells can support sustained waves of dynamically-coupled Rho activity and actin assembly, and mathematical modeling followed by experimental verification revealed that cortical wave propagation is based on Rho autoactivation and actin-mediated Rho inactivation. This constitutes the basis of an "excitable" system, a family of well-established theoretical models with few previously-known cellular manifestations. This project will deduce and experimentally validate a minimal molecular mechanism and basic design elements required for cortical excitability. To achieve the goals of this project, a novel semi-synthetic platform for replicating complex cell behaviors using simple macromolecular parts will be employed, enabling manipulation of the molecular network at escalating complexity instead of by dissecting a complex physiological network. The project will couple computational modeling of excitable dynamics to live-cell imaging in whole cells and in a new ex vivo model of cortical dynamics. First, excitable dynamics will be reconstituted in resting, non-mitotic cells (oocytes of frogs and echinoderms) using natural regulators and their mutants, followed by their replacement with synthetic equivalents. Second, a quantifiable ex vivo model will be developed (using frog oocyte or egg extracts and supported lipid bilayers) that permits precise control of system composition in a simplified context. Third, to achieve on-demand control of cortical pattern formation, an optogenetic approach in animal oocytes will be used to explore the repertoire of both natural and synthetic cortical pattern formation. Iterative experimentation and computational modeling will be employed throughout the project to 1) interpret biological data, 2) express candidate mechanisms in the form of mathematical models, 3) generate predictions, and 4) test those predictions experimentally.