Decoding microtubule remodelling in sperm production

所有真核细胞都有一个动态的微管(MT)细胞骨架，它需要不断的重塑才能满足其许多重要的细胞角色。新出现的数据表明，对MT表面(微管蛋白编码)的修改可能会成为重塑的指导性标志。这个项目的目的是定义微管蛋白编码的一个基本组成部分，谷氨酰化，并定义它如何指导MT的切断。它还旨在确定MT切断酶FIGNL1和关键的MT谷氨酰化酶(CCP1、CCP5和TTLL1)的细胞功能。除了与真核生物的细胞生物学相关外，还将利用精子生产作为模型系统来产生见解，从而为构建可育精子的机制提供信息。

All eukaryotic cells possess a dynamic microtubule (MT) cytoskeleton, which requires constant remodelling to satisfy its many essential cellular roles. Emerging data suggests modifications to the MT surface (the tubulin code) may act as instructional signposts for remodelling. This project aims to define a fundamental component of the tubulin code, glutamylation, and define how this directs MT severing. It also aims to define the cellular functions of MT-severing enzyme FIGNL1 and key MT glutamylation enzymes (CCP1, CCP5 and TTLL1). Insights will be generated using sperm production as a model system and will thus inform the mechanisms by which fertile sperm are built, in addition to being relevant to cell biology across eukaryotic species.