Decoding microtubule remodelling in sperm production

解码精子生成中的微管重塑

• 批准号: DP240100815
• 负责人: Prof Moira O'Bryan
• 金额: $ 46.86万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2024
• 资助国家: 澳大利亚
• 起止时间: 2024-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP240100815
• 关键词: Decoding; microtubule; remodelling; sperm; production

All eukaryotic cells possess a dynamic microtubule (MT) cytoskeleton, which requires constant remodelling to satisfy its many essential cellular roles. Emerging data suggests modifications to the MT surface (the tubulin code) may act as instructional signposts for remodelling. This project aims to define a fundamental component of the tubulin code, glutamylation, and define how this directs MT severing. It also aims to define the cellular functions of MT-severing enzyme FIGNL1 and key MT glutamylation enzymes (CCP1, CCP5 and TTLL1). Insights will be generated using sperm production as a model system and will thus inform the mechanisms by which fertile sperm are built, in addition to being relevant to cell biology across eukaryotic species.

所有真核细胞都具有动态微管（MT）细胞骨架，需要不断重塑以满足其许多重要的细胞功能。新出现的数据表明，修改MT表面（微管蛋白代码）可能作为指导路标重塑。这个项目的目的是定义微管蛋白编码的一个基本组成部分，谷氨酰化，并定义它如何指导MT切断。它还旨在定义MT切断酶FIGNL 1和关键MT谷氨酰化酶（CCP 1，CCP 5和TTLL 1）的细胞功能。将使用精子生产作为模型系统产生见解，从而为建立可育精子的机制提供信息，此外还与真核生物物种的细胞生物学相关。