MRI: Acquisition of Multi-Mode Microplate Detection System for the Multidisciplinary Research Track at Molloy College

MRI：为莫洛伊学院的多学科研究方向采购多模式微孔板检测系统

• 批准号: 1626093
• 负责人: Jodi Evans
• 金额: $ 12.18万
• 依托单位: MOLLOY UNIVERSITY
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1626093&HistoricalAwards=false
• 关键词: MRI; Acquisition; Multi; Mode; Microplate

An award is made to Molloy College to acquire a multimode platform detection system with Western blot and imaging cytometry capabilities. The instrument will be a vital part of several research projects representing a broad cross section of interests including molecular genetics, immunology, molecular biology, cell cycle biology, cellular physiology, developmental biology and environmental science. Biology and environmental studies students participate in these research projects as part of their education and training at Molloy. The students present their findings at regional and national conferences and publish in peer-reviewed journals. High school students are incorporated into the research groups through a high school internship program allowing for undergraduates to gain valuable mentoring experience. The high school students enter local, regional and national science competitions in line with Molloy's mission-directed goal of assisting high school districts in promoting a keen interest in science among their student body. In addition to research activities, the equipment will be incorporated into several courses in the Biology, Chemistry and Environmental Studies Department which will expose a majority of the biology and environmental studies majors as well as the biology education majors of the College to the many experimental techniques supported by absorbance, fluorescence, and luminescence detection. The use of this equipment in these research and training activities directly addresses the National Science Foundation's goal of increasing the use of modern state-of-the-art research equipment by scientists and undergraduate students. Additionally, the STEM initiatives currently in place at Molloy will be strengthened and area students will be provided with advanced training in science that will ultimately make them more competitive for summer positions, research internships, civic engagement opportunities and careers in the biological sciences. The multimode detection system will be used to augment ongoing and planned research projects that further understanding of the 1) mammalian immune system and clarify the role of the stem cell in this system 2) role of highly evolutionarily conserved hormonal systems in the regulation of bone and cartilage development and growth 3) biological role of BLM protein in maintenance of genomic stability in multicellular organisms 4) cell-death pathways initiated after DNA damage and 5) inflammation mechanisms activated by the milk protein, Beta-casomorphin 7. The enhancement of these research efforts at Molloy will in turn raise the research profile of the students, faculty and College.

莫洛伊学院获得了一个具有蛋白质印迹和成像细胞仪功能的多模式平台检测系统的奖项。 该仪器将是代表广泛兴趣的几个研究项目的重要组成部分，包括分子遗传学，免疫学，分子生物学，细胞周期生物学，细胞生理学，发育生物学和环境科学。生物学和环境研究的学生参加这些研究项目，作为他们在莫洛伊教育和培训的一部分。学生们在区域和国家会议上展示他们的研究结果，并在同行评议的期刊上发表。高中生通过高中实习计划被纳入研究小组，让本科生获得宝贵的指导经验。高中生参加地方，地区和国家科学比赛符合莫洛伊的使命为导向的目标，协助高中学区促进学生对科学的浓厚兴趣。除了研究活动，该设备将被纳入生物学，化学和环境研究系的几门课程，这将使大多数生物学和环境研究专业以及学院的生物教育专业的学生接触到由吸光度，荧光和发光检测支持的许多实验技术。在这些研究和培训活动中使用这些设备直接解决了国家科学基金会的目标，即增加科学家和本科生使用现代最先进的研究设备。 此外，目前在莫洛伊实施的STEM计划将得到加强，该地区的学生将获得科学方面的高级培训，最终使他们在暑期职位、研究实习、公民参与机会和生物科学职业方面更具竞争力。多模式检测系统将用于增强正在进行的和计划中的研究项目，进一步了解1）哺乳动物免疫系统，并阐明干细胞在该系统中的作用2）高度进化保守的激素系统在调节骨和软骨发育和生长中的作用3）BLM蛋白在维持多细胞生物体基因组稳定性中的生物学作用4）细胞-DNA损伤后启动的死亡途径和5）由乳蛋白β-酪啡肽7激活的炎症机制。在莫洛伊这些研究工作的增强将反过来提高学生，教师和学院的研究形象。

项目成果

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

间充质干细胞对巨噬细胞吞噬作用的调节；

• DOI: 10.3791/62729
• 发表时间: 2021
• 期刊: Journal of Visualized Experiments
• 作者: Evans, Jodi F.;Ricigliano, Anthony E.;Morante, Anthony V.;Martinez, Emily;Vargas, Darlisy;Thyagaraj, Jonah
• 通讯作者: Thyagaraj, Jonah