MRI: Acquisition of High Resolution Exact Mass Liquid Chromatograph Mass Spectrometer (LCMS) to Broaden and Enhance Chemical and Biological Education and Research

凭借重大研究仪器计划 (MRI) 的这一奖项以及化学研究仪器计划 (CRIF) 以及刺激竞争性研究实验计划 (EPSCoR) 的支持，俄克拉荷马大学诺曼校区的 Robert Cichewicz 教授及其同事 Laura Bartley、Marc Libault、Zhibo Yang 和 Si Wu 获得了一台具有超高性能液相色谱分析功能的高分辨率精确质量液相色谱质谱仪 (LCMS)。 （UPLC）能力。一般来说，质谱 (MS) 是用于识别和表征复杂基质中少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。带有液相色谱仪的仪器通过在化合物混合物到达质谱仪之前对其进行分离来提供额外的结构识别能力。此次收购加强了大学和地区的研究基础设施。该仪器通过使用这种现代分析技术让不同的学生参与研究和研究培训来扩大参与范围。它还通过各种实习项目，如 STEM-to-Store Academy 和 Sooner Upward Bound ，为大量本科生、研究生和博士后以及高中生提供培训机会。该提案旨在加强各个层面的研究和教育，特别是在以下领域：(a) 分析单细胞中的化学和生化物种； (b) 研究单个植物根毛细胞内部生物分子因环境压力而发生的变化； (c) 绘制生物组织中分子的空间分布图； (d) 研究草茎代谢物的空间分布，以研究细胞壁的发育； (e) 表征控制豆类结瘤的调控网络的蛋白质组学，例如检测参与结瘤的转录因子结合伙伴网络； (f) 分析植物干细胞壁合成和调节的代谢物； (g) 分析真菌分泌组的生物质降解酶的蛋白质组学，以及 (h) 量化真菌天然产物化学圈的多样性。

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation Program (CRIF) as well as the Experimental Program to Stimulate Competitive Research (EPSCoR), Professor Robert Cichewicz from the University of Oklahoma Norman Campus and colleagues Laura Bartley, Marc Libault, Zhibo Yang and Si Wu have acquired a high resolution exact mass liquid chromatograph mass spectrometer (LCMS) with ultra performance liquid chromatograhic (UPLC) capabilities. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse students in research and research training using this modern analytical technique. It also provides training opportunities to a large number of undergraduate, graduate and postdoctoral students as well as high school students through a variety of internship programs, such as the STEM-to-Store Academy and Sooner Upward Bound.The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) analyzing chemical and biochemical species in single cells; (b) studying changes to the biomolecules inside of single plant root hair cells in response to environmental stresses; (c) mapping the spatial distribution of molecules in biological tissues; (d) studying the spatial distribution of metabolites of grass stems to investigate cell wall development; (e) characterizing the proteomics of regulatory networks controlling legume nodulation such as detecting the transcription factor binding partner networks involved in nodulation; (f) analyzing the metabolites of plant stem cell wall synthesis and regulation; (g) analyzing the proteomics of fungal secretomes for biomass degrading enzymes and (h) quantifying the diversity of the fungal natural product chemosphere.