RAPID: Microbiome Analysis of Household Biofilm Specimens in Flint, Michigan

RAPID：密歇根州弗林特家庭生物膜样本的微生物组分析

• 批准号: 1639066
• 负责人: Paul Kilgore
• 金额: $ 4.5万
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-15 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1639066&HistoricalAwards=false
• 关键词: RAPID; Microbiome; Analysis; Household; Biofilm

1639066KilgoreIn April 2014, the city of Flint switched its water supply from Detroit's system (Lake Huron) to the Flint River as a cost-saving measure. The increased corrosiveness resulted in the release of heavy metals (e.g., lead and iron) into the water supply. This RAPID grant addresses a critical gap in our knowledge and offers a timely, yet unique opportunity to advance our fundamental understanding of bacterial communities that make up biofilms in the Flint potable water distribution systems.Biofilm and amoebic hosts are an important habitat for pathogens and protect them from disinfectants such as chlorine. Individuals may be exposed to aerosolized Legionella spp., Mycobacterium spp., and Pseudomonas spp in contaminated water from shower heads, shower plumbing, and water heaters. Despite recognition that contaminated water exposure is an important risk factor for Legionellosis, there is currently limited information on bacterial populations that coexist with Legionella spp. within biofilm microbiomes. In this project, the PIs proposed research will characterize bacterial biofilm communities and their distribution within potable water distribution systems supplying Flint households. Using metagenomic analysis targeting 16S rDNA, the PIs hypothesize that biofilm bacterial populations may vary by household distance from water treatment sites as well as by physico-chemical characteristics of water collected concurrent with biofilm sampling. Currently, household sampling of potable water and biofilm swabs from household shower pipes and humidifiers are being analyzed using standard culture and molecular methods for the presence of Legionella spp. The aim of this study is to compare microbiome found in biofilm specimens with and without Legionella spp. The study will utilize quantitative PCR and DNA sequencing methods to identify bacterial pathogens biofilm microbiomes. The proposed project will provide novel characterization of bacterial species in biofilm microbiomes of Flint households. These data will be used to compare potable water chlorine levels among specimens with and without Legionella spp. Results from microbiome analysis will used to guide future environmental sampling and surveillance for Legionella and other bacterial pathogens. Moreover, available Legionella spp. isolated from case-patients in the epidemic of 2015 will be genetically compared with those identified among biofilm specimens in Flint households. The results of this work will help guide the development of important intervention strategies to reduce OPPP exposure and protect susceptible individuals from these potential pathogens.

1639066Kilgore 2014 年 4 月，作为节省成本的措施，弗林特市将供水系统从底特律系统（休伦湖）切换到弗林特河。 腐蚀性增加导致重金属（例如铅和铁）释放到供水中。这笔快速拨款填补了我们知识中的一个关键空白，并提供了一个及时而独特的机会，以增进我们对弗林特饮用水分配系统中构成生物膜的细菌群落的基本了解。生物膜和阿米巴宿主是病原体的重要栖息地，并保护它们免受氯等消毒剂的侵害。个人可能会接触到来自淋浴喷头、淋浴管道和热水器的受污染水中的雾化军团菌、分枝杆菌和假单胞菌。尽管人们认识到接触受污染的水是军团菌病的一个重要危险因素，但目前有关与军团菌共存的细菌种群的信息有限。生物膜微生物组内。在该项目中，PI 提议的研究将描述细菌生物膜群落及其在为弗林特家庭供水的饮用水分配系统中的分布。使用针对 16S rDNA 的宏基因组分析，PI 假设生物膜细菌种群可能因家庭与水处理地点的距离以及生物膜采样同时收集的水的物理化学特征而异。目前，正在使用标准培养和分子方法对家庭饮用水采样以及家庭淋浴管和加湿器的生物膜拭子进行分析，以确定军团菌的存在。本研究的目的是比较含有和不含军团菌的生物膜样本中发现的微生物组。该研究将利用定量 PCR 和 DNA 测序方法来识别细菌病原体生物膜微生物组。拟议的项目将提供弗林特家庭生物膜微生物组中细菌物种的新特征。这些数据将用于比较含有和不含军团菌的样本之间的饮用水氯含量。微生物组分析的结果将用于指导未来军团菌和其他细菌病原体的环境采样和监测。此外，还有可用的军团菌。将从 2015 年疫情中的病例患者中分离出的病毒与弗林特家庭生物膜标本中鉴定出的病毒进行基因比较。这项工作的结果将有助于指导重要干预策略的制定，以减少 OPPP 暴露并保护易感个体免受这些潜在病原体的侵害。