PFI: AIR-TT: Microwave flow cytometer: monitor yeast cell growth and microbial contamination in fermentation
PFI:AIR-TT:微波流式细胞仪:监测发酵过程中的酵母细胞生长和微生物污染
基本信息
- 批准号:1640578
- 负责人:
- 金额:$ 20万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-09-15 至 2019-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This PFI: AIR Technology Translation project focuses on translating tunable microwave interferometer technology to fill the need for online monitoring of yeast cells and early detection of microbial contamination in fermentation processes. The proposed microwave flow cytometer (µFC) is important because it provides essential information to optimize the fermentation process, improve production yield, monitor microbial contamination, and control product quality in various industries, including the bioethanol fuel, brewing and distilling industries. American companies in these industries support millions of jobs and produce several hundred billion dollars' worth of economic outputs annually. Improving productivity and reducing financial loss caused by microbial contaminations are critical for their continued prosperity and growth. The microwave flow cytometer has the following unique features: reagent free cell detection and identification, robust operation, and user friendly instrumentation. These features provide the following advantages: low cost operation, online counting of viable and nonviable yeast cells, and early detection of microbial contamination when compared to the leading competing technologies in the market space, such as optical density (OD) detection, radio-frequency biomass sensing, microscopy based cell counting, staining based viability evaluation, and chemical reaction based yeast activity monitoring.This project will result in a proof-of-concept demonstration of microwave flow cytometer technology for accurately counting yeast cells (cells/mL) at high speed, determining yeast physiological state (viability and vitality), and identifying typical wild yeasts and bacteria in typical fermentation processes. A preliminary database of the microwave properties and signatures of these microorganisms will be obtained. This project will address the following knowledge and technology gaps: the microwave properties and signatures of typical fermentation yeasts and bacteria, the appropriate µFC frequencies for individual yeast cell and bacteria detection and differentiation, and the techniques for the design, fabrication and operation of microwave flow cytometers. In addition, personnel involved in this project, including undergraduate and graduate students, will receive innovation experience through characterizing the microwave properties of yeast cells and identifying their signatures. The students will receive technology translation experience through developing user friendly microwave flow cytometer instruments. They will also receive entrepreneurship experience through close interactions with the industrial partners of the project.This project engages Ferm Solutions and Wilderness Trail Distillery in Danville, KY to provide test environment and commercialization guidance in this technology translation effort from research discovery toward commercial reality.
该PFI:AIR技术转化项目的重点是转化可调谐微波干涉仪技术,以满足发酵过程中酵母细胞在线监测和微生物污染早期检测的需求。 拟议的微波流式细胞仪(µFC)非常重要,因为它提供了优化发酵过程、提高产量、监测微生物污染和控制各种行业(包括生物乙醇燃料、酿造和蒸馏行业)产品质量的基本信息。 这些行业的美国公司每年提供数百万个就业机会,创造价值数千亿美元的经济产出。 提高生产力和减少微生物污染造成的经济损失对它们的持续繁荣和增长至关重要。 微波流式细胞仪具有以下独特的功能:无需试剂的细胞检测和识别,稳健的操作和用户友好的仪器。 这些功能具有以下优点:低成本操作,活的和非活的酵母细胞的在线计数,以及与市场空间中的领先竞争技术相比时的微生物污染的早期检测,例如光密度(OD)检测,射频生物量感测,基于显微镜的细胞计数,基于染色的活力评估,和化学反应为基础的酵母活性监测。该项目将导致微波流式细胞仪技术的概念验证示范,用于准确计数酵母细胞(cells/mL),测定酵母的生理状态(生存力和活力),并在典型的发酵过程中鉴定典型的野生酵母和细菌。 将获得这些微生物的微波特性和特征的初步数据库。 该项目将解决以下知识和技术差距:典型发酵酵母和细菌的微波特性和特征,用于单个酵母细胞和细菌检测和区分的适当µFC频率,以及微波流式细胞仪的设计,制造和操作技术。 此外,参与该项目的人员,包括本科生和研究生,将通过表征酵母细胞的微波特性和识别其特征来获得创新经验。 学生将通过开发用户友好的微波流式细胞仪仪器获得技术翻译经验。 他们还将通过与该项目的工业合作伙伴的密切互动获得创业经验。该项目聘请了位于肯塔基州丹维尔的Ferm Solutions和Wilderness Trail Distillery,为这项从研究发现到商业现实的技术转化工作提供测试环境和商业化指导。
项目成果
期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
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Pingshan Wang其他文献
A rigid metallohexameric macrocycle composed of endo- and exo-cyclic bisterpyridine-metal complexes
由内环和外环双三吡啶金属配合物组成的刚性金属六聚大环
- DOI:
10.1039/c1nj20195f - 发表时间:
2011 - 期刊:
- 影响因子:3.3
- 作者:
Sinan Li;C. Moorefield;Carol D. Shreiner;Pingshan Wang;Rajarshi Sarkar;G. Newkome - 通讯作者:
G. Newkome
Direct Current Effects on High-Frequency Properties of Patterned Permalloy Thin Films
直流电对图案化坡莫合金薄膜高频性能的影响
- DOI:
10.1109/tmag.2009.2024753 - 发表时间:
2009 - 期刊:
- 影响因子:2.1
- 作者:
Hanqiao Zhang;A. Hoffmann;R. Divan;Pingshan Wang - 通讯作者:
Pingshan Wang
Giant Expanded Porous Metallo-Hexagons.
巨型膨胀多孔金属六边形。
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:15
- 作者:
Zhilong Jiang;Bangtang Chen;He Zhao;Jun Wang;Qiangqiang Dong;Fan Fu;Die Liu;Yiming Li;G. Newkome;Pingshan Wang;Mingzhao Chen - 通讯作者:
Mingzhao Chen
Formation of a Series of [{Methylphenylenebis(terpyridine)}nRuIIn–1] (n = 2–6) Oligomers in a Single‐Pot Reaction
在单锅反应中形成一系列 [{甲基亚苯基双(三联吡啶)}nRuIIn–1] (n = 2–6) 低聚物
- DOI:
10.1002/ejoc.200600296 - 发表时间:
2006 - 期刊:
- 影响因子:2.8
- 作者:
T. J. Cho;C. Moorefield;Seok;Pingshan Wang;L. A. Godínez;E. Bustos;G. Newkome - 通讯作者:
G. Newkome
Reducing power-supply and ground noise induced timing jitter in short pulse generation circuits
减少短脉冲生成电路中电源和接地噪声引起的定时抖动
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
Jiwei Sun;Pingshan Wang;Hanqiao Zhang - 通讯作者:
Hanqiao Zhang
Pingshan Wang的其他文献
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{{ truncateString('Pingshan Wang', 18)}}的其他基金
I-Corps: Microwave flow cytometer for real-time monitoring of fermentation
I-Corps:用于实时监测发酵的微波流式细胞仪
- 批准号:
1928967 - 财政年份:2019
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
Broadband microwave flow cytometry: comprehensive nanoparticle sensing and characterization
宽带微波流式细胞术:综合纳米颗粒传感和表征
- 批准号:
1711463 - 财政年份:2017
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
I-Corps Team: Ultra Dielectric Probes
I-Corps 团队:超介电探针
- 批准号:
1539688 - 财政年份:2015
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
Instrument Development: Ultra-Sensitive, Single-Pass Electron Paramagnetic Resonance Spectrometers
仪器开发:超灵敏单通道电子顺磁共振波谱仪
- 批准号:
1152892 - 财政年份:2012
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
Broadband dielectric spectrometers with 1-10 nm planar nanofluidic channels
具有 1-10 nm 平面纳米流体通道的宽带介电谱仪
- 批准号:
0925424 - 财政年份:2009
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
Network Analyzer on Chip: An Integrated Frequency Domain Sensor
片上网络分析仪:集成频域传感器
- 批准号:
0703042 - 财政年份:2006
- 资助金额:
$ 20万 - 项目类别:
Continuing Grant
Network Analyzer on Chip: An Integrated Frequency Domain Sensor
片上网络分析仪:集成频域传感器
- 批准号:
0622082 - 财政年份:2006
- 资助金额:
$ 20万 - 项目类别:
Continuing Grant
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