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Viral control of microbial communities in Antarctic lakes

南极湖泊微生物群落的病毒控制

基本信息

批准号：
1644155
负责人：
Benjamin Twining
金额：
$ 49.82万
依托单位：
Bigelow Laboratory for Ocean Sciences
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2022-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1644155&HistoricalAwards=false
关键词：
Viral control microbial communities Antarctic

项目摘要

Viruses are prevalent in aquatic environments where they reach up to five hundred million virus particles in a teaspoon of water. Ongoing discovery of viruses seems to confirm current understanding that all forms of life can host and be infected by viruses and that viruses are one of the largest reservoirs of unexplored genetic diversity on Earth. This study aims to better understand interactions between specific viruses and phytoplankton hosts and determine how these viruses may affect different algal groups present within lakes of the Vestfold Hills, Antarctica. These lakes (Ace, Organic and Deep)were originally derived from the ocean and contain a broad range of saline conditions with a similarly broad range of physicochemical characteristics resulting from isolation and low external influence for thousands of years. These natural laboratories allow examination of microbial processes and interactions that would be difficult to characterize elsewhere on earth. The project will generate extensive genomic information that will be made freely available. The project will also leverage the study of viruses and the genomic approaches employed to advance the training of undergraduate students and to engage and foster an understanding of Antarctic science and studies of microbes during a structured informal education program in Maine for the benefit of high school students. By establishing the dynamics and interactions of (primarily) specific dsDNA virus groups in different habitats with different redox conditions throughout seasonal and inter annual cycles the project will learn about the biotic and abiotic factors that influence microbial community dynamics. This project does not require fieldwork in Antarctica. Instead, the investigators will leverage already collected and archived samples from three lakes that have concurrent measures of physicochemical information. Approximately 2 terabyte of Next Generation Sequencing (NGS) (including metagenomes, SSU rRNA amplicons and single virus genomes) will be generated from selected available samples through a Community Science Program (CSP) funded by the Joint Genome Institute. The investigators will employ bioinformatics to interrogate those sequence databases. In particular, they will focus on investigating the presence, phylogeny and co-occurrence of polintons, polinton-like viruses, virophages and large dsDNA phytoplankton viruses as well as of their putative eukaryotic microbial hosts. Bioinformatic analyses will be complemented with quantitative digital PCR and microbial association network analysis to detect specific virus?virus?host interactions from co-occurrence spatial and temporal patterns. Multivariate analysis and network analyses will also be performed to investigate which abiotic factors most closely correlate with phytoplankton and virus abundances, temporal dynamics, and observed virus-phytoplankton associations within the three lakes. The results of this project will improve understanding of phytoplankton and their viruses as vital components of the carbon cycle in Antarctic, marine-derived aquatic environments, and likely in any other aquatic environment. Overall, this work will advance understanding of the genetic underpinnings of adaptations in unique Antarctic environments.

病毒在水生环境中普遍存在，一茶匙水中的病毒颗粒多达五亿个。病毒的不断发现似乎证实了目前的认识，即所有形式的生命都可以宿主并被病毒感染，病毒是地球上未探索的遗传多样性的最大储存库之一。本研究旨在更好地了解特定的病毒和浮游植物宿主之间的相互作用，并确定这些病毒可能会影响不同的藻类群体内存在的韦斯特福尔山，南极洲的湖泊。这些湖泊（Ace，Organic和Deep）最初来自海洋，含有广泛的盐水条件，具有类似的广泛的物理化学特性，这些特性是数千年来隔离和低外部影响造成的。这些天然实验室允许检查微生物过程和相互作用，这在地球上的其他地方很难描述。该项目将产生广泛的基因组信息，并将免费提供。该项目还将利用对病毒的研究和基因组方法来推进本科生的培训，并在缅因州的结构化非正式教育计划中参与和促进对南极科学和微生物研究的理解，以造福高中生。通过在不同的季节和年际周期中建立不同生境中（主要是）特定dsDNA病毒群的动态和相互作用，该项目将了解影响微生物群落动态的生物和非生物因素。该项目不需要在南极洲进行实地考察。相反，研究人员将利用从三个湖泊中收集和存档的样本，这些样本具有同步的物理化学信息测量。将通过联合基因组研究所资助的社区科学计划（CSP）从选定的可用样本中生成约2 TB的下一代测序（NGS）（包括宏基因组，SSU rRNA扩增子和单病毒基因组）。研究人员将使用生物信息学来查询这些序列数据库。特别是，他们将专注于调查的存在，繁殖和共同出现的polintons，polinton样病毒，病毒和大dsDNA浮游植物病毒，以及他们假定的真核微生物宿主。生物信息学分析将补充定量数字PCR和微生物关联网络分析，以检测特定的病毒？病毒？从共生的空间和时间模式的主机相互作用。还将进行多变量分析和网络分析，以调查哪些非生物因素与浮游植物和病毒丰度、时间动态以及观察到的三个湖泊内的病毒-浮游植物协会最密切相关。该项目的结果将提高对浮游植物及其病毒作为南极、海洋衍生水生环境以及可能在任何其他水生环境中碳循环的重要组成部分的认识。总的来说，这项工作将促进对南极独特环境中适应的遗传基础的理解。