EAGER: Scanningless 3D Bioprinting of Multiple Biomaterials and Cells for Biomimetic Vascular Network

EAGER：用于仿生血管网络的多种生物材料和细胞的非扫描 3D 生物打印

基本信息

批准号：
1644967
负责人：
Shaochen Chen
金额：
$ 30万
依托单位：
University of California-San Diego
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2020-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1644967&HistoricalAwards=false
关键词：
EAGER Scanningless 3D Bioprinting Multiple

项目摘要

Vascularization has been the bottleneck for engineering large-scale or highly metabolic tissues. Without vascular support, cellular viability and function of engineered tissues or organs will be compromised in very short time. Traditional biomanufacturing methods such as nozzle-based and ink-jet based 3D printing are often slow and have limited printing resolution for creating biomimetic vasculature. This EArly-concept Grant for Exploratory Research (EAGER) award supports fundamental research on a new biomanufacturing method that simultaneously offers the speed, the resolution, and the ability to process multiple biomaterials and cells to 3D print biomimetic vascular network. Results from this research will potentially transform the biomanufacturing field for future tissue and organ printing with biomimetic vascular network. Printing organs such as heart and liver will reduce the shortage of donor organs for transplantations and save lives. Additionally, the biomimetic in vitro tissue models could significantly benefit the pharmatheutical industry because they can be used in early drug screening for drug toxicity and efficacy testing.The new biomanufacturing method features ultraviolet light-induced hydrogel formation in a scanningless and continuous fashion for rapid 3D printing of biomimetic vascular network. The first research objective is to understand the effects of material composition and processing parameters on mechanical properties of the hydrogel scaffolds for the 3D printing process. To achieve this objective, glycidal methacrylate-hyaluronic acid and gelatin methacrylate will be synthesized as the hydrogel materials with different methacrylation ratios. Hydrogel scaffolds will be printed by varying material composition (such as molecular weight and concentration of the monomers) and processing parameters (such as ultraviolet light intensity and exposure time). Mechanical properties (such as stiffness and yield strength) of printed hydrogel scaffolds will be measured by a nanoindentor and dynamic mechanical analyzer. The second objective is to understand how scaffold shape and chemistry affect vascular network formation. To achieve this objective, scaffolds of different shapes including single tubes and branched tubes will be printed using different hydrogels (glycidal methacrylate-hyaluronic acid and gelatin methacrylate). Human umbilical vein endothelial cells and mesenchymal stem cells will be encapsulated in the hydrogels to form a vascularized tissue. Vascular network formation such as lumens will be imaged using confocal microscopy.

血管化一直是工程大规模或高度代谢组织的瓶颈。如果没有血管支持，工程组织或器官的细胞活力和功能将在很短的时间内被损害。传统的生物制造方法，例如基于喷嘴的和基于喷墨的3D打印通常很慢，并且印刷分辨率有限，用于创建仿生脉管系统。这项探索性研究的早期概念赠款（急切）奖支持了一种基本研究，该研究同时提供了速度，分辨率以及处理多个生物材料和细胞的能力，以至于3D印刷仿生血管网络。这项研究的结果将有可能改变使用仿生血管网络的未来组织和器官印刷的生物制造场。诸如心脏和肝脏等印刷器官将减少供体器官的短缺移植并挽救生命。此外，生物仿生体体内组织模型可以显着受益于药物行业，因为它们可用于药物毒性和功效测试的早期药物筛查。新的生物制造方法具有紫外线诱导的水凝胶形成，以扫描和连续的方式用于生物象征性血管网络的快速3D打印。第一个研究目标是了解材料组成和加工参数对3D打印过程中水凝胶支架机械性能的影响。为了实现这一目标，将乙二醇甲基丙烯酸酰酸酸和明胶甲基丙烯酸酯合成为具有不同甲基丙烯酸酯比的水凝胶材料。水凝胶支架将通过不同的材料组成（例如分子量和单体的浓度）和加工参数（例如紫外线的光强度和暴露时间）来打印。印刷水凝胶支架的机械性能（例如刚度和屈服强度）将通过纳米INDENTOR和动态机械分析仪进行测量。第二个目标是了解脚手架形状和化学如何影响血管网络形成。为了实现这一目标，将使用不同的水凝胶（糖甲基丙烯酸甲酯 - 透明甲酸和明胶甲基丙烯酸酯）打印不同形状的支架。人脐静脉内皮细胞和间质干细胞将封装在水凝胶中，形成血管化组织。血管网络形成（例如流明）将使用共聚焦显微镜成像。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Controlled Growth Factor Release in 3D‐Printed Hydrogels

DOI：
10.1002/adhm.201900977
发表时间：
2019-11
期刊：
Advanced Healthcare Materials
影响因子：
10
作者：
Pengrui Wang;D. Berry;A. Moran;F. He;Trevor Tam;Luwen Chen;Shaochen Chen
通讯作者：
Pengrui Wang;D. Berry;A. Moran;F. He;Trevor Tam;Luwen Chen;Shaochen Chen

Direct 3D bioprinting of cardiac micro-tissues mimicking native myocardium

DOI：
10.1016/j.biomaterials.2020.120204
发表时间：
2020-10-01
期刊：
BIOMATERIALS
影响因子：
14
作者：
Liu, Justin;Miller, Kathleen;Chen, Shaochen
通讯作者：
Chen, Shaochen

Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix

DOI：
10.1016/j.biomaterials.2018.12.009
发表时间：
2019-02-01
期刊：
BIOMATERIALS
影响因子：
14
作者：
Yu, Claire;Ma, Xuanyi;Chen, Shaochen
通讯作者：
Chen, Shaochen

A 3D Tissue-Printing Approach for Validation of Diffusion Tensor Imaging in Skeletal Muscle

DOI：
10.1089/ten.tea.2016.0438
发表时间：
2017-09-01
期刊：
TISSUE ENGINEERING PART A
影响因子：
4.1
作者：
Berry, David B.;You, Shangting;Ward, Samuel R.
通讯作者：
Ward, Samuel R.

3D-Printing of Functional Biomedical Microdevices via Light- and Extrusion-Based Approaches

DOI：
10.1002/smtd.201700277
发表时间：
2018-02-13
期刊：
SMALL METHODS
影响因子：
12.4
作者：
Hwang, Henry H.;Zhu, Wei;Chen, Shaochen
通讯作者：
Chen, Shaochen

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Shaochen Chen其他文献

Toward a 3D bio-printed model of placental-villous transport: Growth and viability of human primary placental cell-types on hydrogel scaffolds

DOI：
10.1016/j.placenta.2019.06.341
发表时间：
2019-08-01
期刊：
Conference abstract
影响因子：
作者：
Chandana Tekkatte;Claire Yu;Xuanyi Ma;Henry H. Hwang;Omar Farah;Ching-Wen Chang;Mana M. Parast;Shaochen Chen;Louise C. Laurent
通讯作者：
Louise C. Laurent