Molecular, Biochemical, and Structural Studies of the Mechanism of S-RNase-Based Self-Incompatibility in Petunia

矮牵牛基于 S-RNase 的自交不亲和机制的分子、生化和结构研究

基本信息

  • 批准号:
    1645557
  • 负责人:
  • 金额:
    $ 68万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-03-15 至 2023-02-28
  • 项目状态:
    已结题

项目摘要

Most flowering plants produce flowers with the male reproductive organ, the anther, and female reproductive organ, the pistil, located in close proximity. This arrangement would allow the pollen produced by the anther to land on the top of the pistil to result in self-pollination and consequent inbreeding. Inbreeding is deleterious to any organism, as it causes reduced fitness in progeny. Flowering plants have adopted self-incompatibility (SI), which allows the pistil to reject self-pollen to prevent inbreeding, but accept non-self pollen to promote outcrossing. Since Charles Darwin first documented this phenomenon in a monograph published in 1876, SI has attracted the interest of a wide spectrum of biologists. The PI has identified a pistil protein and 17 pollen proteins that are key players in mediating SI in Petunia inflata, a wild relative of garden petunia. In this project, the PI and his collaborator will use a combination of molecular, biochemical, genetic, genomic, and structural approaches to determine how the 17 pollen proteins and a single pistil protein work together to allow the pistil to specifically reject self-pollen. The results have wider implications for the study of many other biological systems employing self/non-self recognition. The genes for these proteins could be used to facilitate hybrid-seed production, which, if accomplished, would provide tremendous agronomic benefits. The PI will engage graduate and undergraduate students in this project to prepare them for future research careers, and will offer summer research opportunity for high school students through Penn State's Upward Bound Math and Science Program. In S-RNase-based self-incompatibility in Petunia inflata, the polymorphic S-locus controls the outcome of pollination; matching of the pollen S-haplotype with either S-haplotype of the pistil results in growth inhibition of pollen tubes. The PI has shown that the S-RNase gene controls pistil specificity and identified 17 S-locus F-box (SLF) genes in S2-haplotype that collectively control pollen specificity. In this project, the PI will complete the determination of 187 interaction relationships between all 17 SLF proteins and 11 allelic variants of S-RNase. These results will be used to study the biochemical basis of differential interactions between SLF proteins and S-RNases, and select SLF/S-RNase pairs for X-ray structural study of the interactions. Bacterial Artificial Chromosome (BAC) clones collectively containing the S-RNase and 17 SLF genes have been isolated, sequenced, and assembled, totaling ~3.1 Mbp. To fill gaps in the S-locus sequence, additional BAC clones will be isolated and sequenced. The PI has shown that all 17 SLF proteins form similar SCF complexes, but the Cullin1 and Skp1 components (PiCUL1-P and PiSSK1) are pollen-specific. The effect of knockout and suppressed expression of PiCUL1-P and PiSSK1 will be examined, and the X-ray structure of the SCFSLF complex will be determined. The PI has found that SLF proteins themselves are subject to ubiquitin-mediated degradation by the 26S proteasome, and identified proteins that may be involved in regulating the dynamics of the SCFSLF complex. The role of these proteins will be examined. All X-ray crystallization experiments will be performed in the lab of the collaborator.
大多数开花植物产生的花与男性生殖器官,花药,和女性生殖器官,雌蕊,位于靠近。 这种排列方式使花药产生的花粉落在雌蕊的顶部,导致自花授粉和随后的近亲繁殖。 近亲繁殖对任何生物体都是有害的,因为它会导致后代适应性的降低。 开花植物采用自交不亲和性(SI),这使得雌蕊拒绝自交花粉以防止近亲繁殖,但接受非自交花粉以促进异交。 自从查尔斯·达尔文在1876年出版的专著中首次记录了这一现象以来,SI吸引了广泛的生物学家的兴趣。 PI已经鉴定了一种雌蕊蛋白和17种花粉蛋白,它们是介导矮牵牛(Petuniainflata)SI的关键分子,矮牵牛是花园矮牵牛的野生亲戚。 在这个项目中,PI和他的合作者将使用分子,生物化学,遗传学,基因组学和结构方法的组合来确定17种花粉蛋白和单一雌蕊蛋白如何共同作用,使雌蕊特异性地拒绝自花花粉。 这些结果对许多其他采用自我/非自我识别的生物系统的研究具有更广泛的意义。 这些蛋白质的基因可用于促进杂交种子的生产,如果实现,将提供巨大的农艺效益。 PI将让研究生和本科生参与这个项目,为他们未来的研究生涯做好准备,并将通过宾夕法尼亚州立大学的向上约束数学和科学计划为高中生提供夏季研究机会。在矮牵牛基于S-RNase的自交不亲和性中,多态性S-位点控制授粉的结果;花粉S-单倍型与雌蕊的任一S-单倍型的匹配导致花粉管的生长抑制。 PI已经表明S-RNase基因控制雌蕊特异性,并在S2单倍型中鉴定了17个共同控制花粉特异性的S-位点F-box(SLF)基因。 在本项目中,PI将完成所有17种SLF蛋白与S-RNase的11种等位基因变体之间的187种相互作用关系的确定。 这些结果将用于研究SLF蛋白和S-RNase之间差异相互作用的生化基础,并选择SLF/S-RNase对进行相互作用的X射线结构研究。 细菌人工染色体(BAC)克隆集体含有S-RNase和17个SLF基因已被分离,测序,并组装,共约3.1 Mbp。 为了填补S基因座序列中的缺口,将分离并测序额外的BAC克隆。 PI已经表明,所有17个SLF蛋白形成类似的SCF复合物,但Cullin 1和Skp 1组分(PiCUL 1-P和PiSSK 1)是花粉特异性的。 将检查PiCUL 1-P和PiSSK 1的敲除和抑制表达的效果,并将确定SCFSLF复合物的X射线结构。 PI发现SLF蛋白本身受到26 S蛋白酶体的泛素介导的降解,并鉴定了可能参与调节SCFSLF复合物动力学的蛋白质。 这些蛋白质的作用将被研究。 所有X射线结晶实验将在合作者的实验室中进行。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Use of Domain-Swapping to Identify Candidate Amino Acids Involved in Differential Interactions between Two Allelic Variants of Type-1 S-Locus F-Box Protein and S3-RNase in Petunia inflata
  • DOI:
    10.1093/pcp/pcx176
  • 发表时间:
    2018-02
  • 期刊:
  • 影响因子:
    4.9
  • 作者:
    Lihua Wu;Justin S. Williams;Ning Wang;Wasi A Khatri;Daniele San Rom[REPLACEMENT CHARACTER]n;T. Kao
  • 通讯作者:
    Lihua Wu;Justin S. Williams;Ning Wang;Wasi A Khatri;Daniele San Rom[REPLACEMENT CHARACTER]n;T. Kao
Analyses of Cullin1 homologs reveal functional redundancy in S-RNase-based self-incompatibility and evolutionary relationships in eudicots
Cullin1 同源物的分析揭示了真双子叶植物中基于 S-RNase 的自交不亲和性和进化关系的功能冗余
  • DOI:
    10.1093/plcell/koac357
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sun, Linhan;Cao, Shiyun;Zheng, Ning;Kao, Teh-hui
  • 通讯作者:
    Kao, Teh-hui
S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen
  • DOI:
    10.1105/tpc.18.00615
  • 发表时间:
    2018-12-01
  • 期刊:
  • 影响因子:
    11.6
  • 作者:
    Sun, Linhan;Williams, Justin S.;Kao, Teh-hui
  • 通讯作者:
    Kao, Teh-hui
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Teh-hui Kao其他文献

Characterization of a pollen-expressed gene encoding a putative pectin esterase ofPetunia inflata
  • DOI:
    10.1007/bf00043881
  • 发表时间:
    1994-06-01
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Jing-Hong Mu;Joseph P. Stains;Teh-hui Kao
  • 通讯作者:
    Teh-hui Kao
A social stigma
社会耻辱
  • DOI:
    10.1038/35002702
  • 发表时间:
    2000-02-24
  • 期刊:
  • 影响因子:
    48.500
  • 作者:
    Teh-hui Kao;Andrew G. McCubbin
  • 通讯作者:
    Andrew G. McCubbin
Isolation and nucleotide sequence of the pea cytochrome oxidase subunit I gene
  • DOI:
    10.1007/bf00027340
  • 发表时间:
    1989-07-01
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Elizabeth C. Kemmerer;Teh-hui Kao;Guo-ren Deng;Ray Wu
  • 通讯作者:
    Ray Wu
Distribution of self-compatible and self-incompatible populations of Petunia axillaris (Solanaceae) outside Uruguay
  • DOI:
    10.1007/s10265-006-0002-y
  • 发表时间:
    2006-08-17
  • 期刊:
  • 影响因子:
    2.300
  • 作者:
    Hisashi Kokubun;Makoto Nakano;Tatsuya Tsukamoto;Hitoshi Watanabe;Goro Hashimoto;Eduardo Marchesi;Laura Bullrich;Isabel L. Basualdo;Teh-hui Kao;Toshio Ando
  • 通讯作者:
    Toshio Ando
Characterization of a predominantly pistil-expressed gene encoding a γ-thionin-like protein of Petunia inflata
  • DOI:
    10.1007/bf00039555
  • 发表时间:
    1994-10-01
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Balasulojini Karunanandaa;Anuradha Singh;Teh-hui Kao
  • 通讯作者:
    Teh-hui Kao

Teh-hui Kao的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Teh-hui Kao', 18)}}的其他基金

Molecular, Biochemical, and Structural Studies of the Mechanism of S-RNase-Based Self-Incompatibility in Petunia
矮牵牛基于 S-RNase 的自交不亲和机制的分子、生化和结构研究
  • 批准号:
    2138062
  • 财政年份:
    2022
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Biochemical Basis of S-RNase-Based Self-Incompatibility
基于 S-RNase 的自交不亲和性的生化基础
  • 批准号:
    1146182
  • 财政年份:
    2012
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Biochemical Basis of S-RNase-Based Self-Incompatibility
基于 S-RNase 的自交不亲和性的生化基础
  • 批准号:
    0843195
  • 财政年份:
    2009
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
The Role of the S-Locus F-Box Gene in S-RNase-Based Self-Incompatibility
S-位点 F-Box 基因在基于 S-RNase 的自交不亲和性中的作用
  • 批准号:
    0543201
  • 财政年份:
    2006
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Molecular Basis of Self-Incompatibility in Petunia
矮牵牛自交不亲和性的分子基础
  • 批准号:
    0235176
  • 财政年份:
    2003
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Molecular Basis of Self-Incompatibility in Petunia
矮牵牛自交不亲和性的分子基础
  • 批准号:
    9982659
  • 财政年份:
    2000
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Molecular Basis of Self-Incompatibility in Petunia
矮牵牛自交不亲和性的分子基础
  • 批准号:
    9603993
  • 财政年份:
    1997
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Molecular Genetic Basis of Self-Incompatibility in Petunia
矮牵牛自交不亲和性的分子遗传学基础
  • 批准号:
    9220145
  • 财政年份:
    1993
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Molecular Basis of Gametophytic Self-Incompatibility in Nicotiana and Petunia
烟草和矮牵牛配子体自交不亲和性的分子基础
  • 批准号:
    8904008
  • 财政年份:
    1990
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Molecular Basis of Gametophytic Self-Incompatibility System in Petunia and Solanum
矮牵牛和茄属配子体自交不亲和系统的分子基础
  • 批准号:
    8616087
  • 财政年份:
    1987
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant

相似海外基金

CAREER: Biochemical and Structural Mechanisms Controlling tRNA-Modifying Metalloenzymes
职业:控制 tRNA 修饰金属酶的生化和结构机制
  • 批准号:
    2339759
  • 财政年份:
    2024
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Structural and biochemical investigations into the mechanism and evolution of soluble guanylate cyclase regulation
可溶性鸟苷酸环化酶调节机制和进化的结构和生化研究
  • 批准号:
    10604822
  • 财政年份:
    2023
  • 资助金额:
    $ 68万
  • 项目类别:
Elucidating the molecular basis and expanding the biological applications of the glycosyltransferases using biochemical and structural biology approaches
利用生化和结构生物学方法阐明糖基转移酶的分子基础并扩展其生物学应用
  • 批准号:
    23K14138
  • 财政年份:
    2023
  • 资助金额:
    $ 68万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Structural and biochemical characterization of VCPIP1 and VCP complex
VCPIP1 和 VCP 复合物的结构和生化表征
  • 批准号:
    10675974
  • 财政年份:
    2023
  • 资助金额:
    $ 68万
  • 项目类别:
Biochemical and structural characterization of the cell wall synthesis complex required for bacterial division
细菌分裂所需的细胞壁合成复合物的生化和结构表征
  • 批准号:
    10750639
  • 财政年份:
    2023
  • 资助金额:
    $ 68万
  • 项目类别:
Multi-Scale Computer Modeling of the Structural Impact and Biochemical Reactions of Damaged DNA
受损 DNA 的结构影响和生化反应的多尺度计算机建模
  • 批准号:
    RGPIN-2016-04568
  • 财政年份:
    2022
  • 资助金额:
    $ 68万
  • 项目类别:
    Discovery Grants Program - Individual
Structural and Biochemical Effects of Capsid-targeting Molecules on HIV-1 Capsid Assembly
衣壳靶向分子对 HIV-1 衣壳组装的结构和生化影响
  • 批准号:
    10619783
  • 财政年份:
    2022
  • 资助金额:
    $ 68万
  • 项目类别:
Molecular, Biochemical, and Structural Studies of the Mechanism of S-RNase-Based Self-Incompatibility in Petunia
矮牵牛基于 S-RNase 的自交不亲和机制的分子、生化和结构研究
  • 批准号:
    2138062
  • 财政年份:
    2022
  • 资助金额:
    $ 68万
  • 项目类别:
    Continuing Grant
Biochemical, structural and molecular dissection of androgen receptor transcriptional activity
雄激素受体转录活性的生化、结构和分子剖析
  • 批准号:
    10655002
  • 财政年份:
    2022
  • 资助金额:
    $ 68万
  • 项目类别:
Develop enabling biochemical and structural tools for dissecting the roles of PKD2L2 in metabolism
开发有利的生化和结构工具来剖析 PKD2L2 在代谢中的作用
  • 批准号:
    10452211
  • 财政年份:
    2022
  • 资助金额:
    $ 68万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了