Biochemical Basis of S-RNase-Based Self-Incompatibility

基于 S-RNase 的自交不亲和性的生化基础

• 批准号: 0843195
• 负责人: Teh-hui Kao
• 金额: $ 46.8万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0843195&HistoricalAwards=false
• 关键词: Biochemical; Basis; RNase; Based; Self

PI: Teh-hui Kao Proposal Number: IOS-0843195Title of Proposal: Biochemical Basis of S-RNase-Based Self-IncompatibilityMost flowering plants produce bisexual flowers containing both male and female reproductive organs, and they would have a strong tendency to self-fertilize, if no mechanisms prevent them from doing so. Since inbreeding is deleterious to any organism, flowering plants have evolved a variety of reproductive strategies, including self-incompatibility (SI), to prevent inbreeding and promote out-crossing. SI allows pistils to reject self-pollen and accept non-self pollen for fertilization. This project uses Petunia inflata to study one type of SI mechanism, and has previously identified the S-RNase expressed by the pistil and PiSLF (P. inflata S-locus F-box) that is expressed by the pollen. During growth of a pollen tube in a pistil, the PiSLF in the pollen tube only allows self S-RNase to inhibit pollen tube growth. Several hypotheses that explain how PiSLF and S-RNase interact to elicit specific rejection of self pollen tubes will be tested. The project will use in vivo approaches to test these hypotheses by dissecting the functions of three domains of PiSLF in interactions with S-RNase, studying the biochemical function of a potential component of the PiSLF-containing complex, and examining the role of glycosylation of S-RNase in SI. SI provides a model for studying self/non-self recognition, so completion of this project will have wider implications for biological sciences. PiSLF and S-RNase could be used for restoring SI to crop species to facilitate hybrid seed production, which will have tremendous agronomic benefits. The project will engage three graduate and three undergraduate students to prepare them for future careers in research. The researchers in this project will continue to participate in an annual biology fair at Penn State to educate the attendees, particularly K-12, about plant reproductive biology and SI.

主要研究者：高德惠提案编号：IOS-0843195提案标题：基于S-RNase的自交不亲和性的生化基础大多数开花植物产生包含雄性和雌性生殖器官的两性花，如果没有机制阻止它们这样做，它们将具有强烈的自花受精倾向。 由于近亲繁殖对任何生物体都是有害的，有花植物已经进化出各种生殖策略，包括自交不亲和性（SI），以防止近亲繁殖和促进异交。 SI允许雌蕊排斥自花花粉而接受非自花花粉进行受精。 本研究以矮牵牛为研究对象，对其中一种SI机制进行了研究，并鉴定了雌蕊表达的S-RNase和花粉表达的PiSLF（P. inflata S-locus F-box）。在花粉管在雌蕊中生长期间，花粉管中的PiSLF仅允许自身S-RNase抑制花粉管生长。 几个假设，解释如何PiSLF和S-RNase相互作用，引起特异性排斥的自我花粉管将被测试。 该项目将使用体内方法来测试这些假设，通过解剖PiSLF的三个结构域与S-RNase相互作用的功能，研究含有PiSLF的复合物的潜在组分的生化功能，并检查S-RNase糖基化在SI中的作用。 SI为研究自我/非自我识别提供了一个模型，因此该项目的完成将对生物科学产生更广泛的影响。 PiSLF和S-RNase可用于恢复作物物种的SI，以促进杂交种子生产，这将具有巨大的农艺效益。 该项目将聘请三名研究生和三名本科生，为他们未来的研究生涯做好准备。该项目的研究人员将继续参加宾夕法尼亚州立大学的年度生物学博览会，以教育与会者，特别是K-12，关于植物生殖生物学和SI。