The molecular life history of male gametes

雄配子的分子生命史

• 批准号: 1655840
• 负责人: Steve Dorus
• 金额: $ 69.76万
• 依托单位: Syracuse University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1655840&HistoricalAwards=false
• 关键词: molecular; life; history; male; gametes

This work will contribute to our understanding of factors affecting fertility and how differences in the interactions between sperm and the reproductive tracts contribute to the reproductive isolation of species. After leaving the testes, sperm undergo numerous changes as they move through the male and female reproductive tracts. These changes to the sperm cells, how they vary among species, and their importance for fertilization success are not well understood. This project will characterize these changes, at the molecular level, within and among closely-related species of fruit fly to understand their evolutionary history, the cause of specific changes, and the effects for sperm survival and fertilization success. This project will also result in the development of a "Genes, Genomes, and Society" (GGS) educational program to introduce high-school students to the field of genomics. It will sponsor a genomics workshop for New York High School teachers to encourage adoption of the GGS program. Finally, some high school students and teachers will also participate in laboratory research. Using whole-cell proteomic analyses, sperm will be tracked, beginning with storage in the male seminal vesicle, through ejaculation and following protracted storage in the primary sperm-storage organ of females. Identical analyses will be conducted using two sibling Drosophila species (D. simulans and D. mauritiana) and for reciprocal hybrid matings between the species, where evolved ejaculate-female compatibility has potentially been compromised. Proteomic analyses will be similarly conducted on female reproductive tract secretions. The resulting datasets will provide, for closely related species, an exhaustive library of the sperm proteome throughout the life history of sperm, which will permit all alterations to sperm (protein gain, loss and modification) to be discerned. With regard to proteins added to sperm during storage, reciprocal sex-specific labeling will distinguish male- from female-contributed proteins. Molecular evolutionary and genomic analyses of proteins involved in sperm-female interactions, together with the data from hybrid inseminations, will address questions about rates of divergence, genetic architecture and the putative role of sperm-female interactions in speciation.

这项工作将有助于我们理解影响生育能力的因素，以及精子和生殖道之间相互作用的差异如何有助于物种的生殖隔离。离开睾丸后，精子在通过男性和女性生殖道时会经历许多变化。精子细胞的这些变化，它们在不同物种之间的差异，以及它们对受精成功的重要性还没有被很好地理解。这个项目将在分子水平上，在果蝇密切相关的物种内部和之间表征这些变化，以了解它们的进化历史，特定变化的原因，以及对精子存活和受精成功的影响。该项目还将开发一个“基因、基因组和社会”(GGS)教育项目，向高中生介绍基因组学领域。它将为纽约高中教师举办基因组学研讨会，以鼓励采用GGS计划。最后，一些高中生和老师也将参加实验室研究。使用全细胞蛋白质组分析，将跟踪精子，从储存在男性精囊中开始，到射精，然后在女性主要精子储存器官中长期储存。同样的分析将使用两个兄弟果蝇物种(模拟果蝇和毛果蝇)，以及物种之间的相互杂交交配，其中进化的射精-雌性兼容性可能已经受到影响。对女性生殖道分泌物的蛋白质组学分析也将同样进行。由此产生的数据集将为密切相关的物种提供一个贯穿精子生活史的详尽的精子蛋白质组文库，这将使人们能够识别精子的所有变化(蛋白质的获得、丢失和修饰)。对于在储存过程中添加到精子中的蛋白质，相互的性别特异性标记将区分男性和女性贡献的蛋白质。对涉及精子-雌性相互作用的蛋白质的分子进化和基因组分析，以及来自杂交受精的数据，将解决关于分化速度、遗传结构和精子-雌性相互作用在物种形成中的假定作用的问题。

项目成果

Quantitative proteomics reveals rapid divergence in the postmating response of female reproductive tracts among sibling species

• DOI: 10.1098/rspb.2020.1030
• 发表时间: 2020-06
• 期刊: Proceedings of the Royal Society B
• 作者: E. McCullough;Caitlin E. McDonough;S. Pitnick;S. Dorus

Molecular Diversification of the Seminal Fluid Proteome in a Recently Diverged Passerine Species Pair

• DOI: 10.1093/molbev/msz235
• 发表时间: 2019-10
• 期刊: Molecular Biology and Evolution
• 影响因子: 10.7
• 作者: Melissah Rowe;E. Whittington;Kirill Borziak;M. Ravinet;F. Eroukhmanoff;Glenn-Peter Sætre;S. Dorus

Female novelty and male status dynamically modulate ejaculate expenditure and seminal fluid proteome over successive matings in red junglefowl

• DOI: 10.1038/s41598-019-41336-5
• 发表时间: 2019-04-10
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Alvarez-Fernandez, Aitor;Borziak, Kirill;Pizzari, Tommaso
• 通讯作者: Pizzari, Tommaso

Evolutionary Proteomics Reveals Distinct Patterns of Complexity and Divergence between Lepidopteran Sperm Morphs

进化蛋白质组学揭示了鳞翅目精子形态之间复杂性和分歧的独特模式

• DOI: 10.1093/gbe/evz080
• 发表时间: 2019
• 期刊: Genome Biology and Evolution
• 影响因子: 3.3
• 作者: Whittington, Emma;Karr, Timothy L;Mongue, Andrew J;Dorus, Steve;Walters, James R;Eyre-Walker, Adam
• 通讯作者: Eyre-Walker, Adam