Galectins of the eastern oyster (C. virginica) and softshell clam (M. arenaria) as determinants for host preference and pathogenicity of sympatric Perkinsus parasite species

东部牡蛎(C. virginica)和软壳蛤(M. arenararia)的半乳糖凝集素作为同域帕金瑟斯寄生虫物种的宿主偏好和致病性的决定因素

基本信息

  • 批准号:
    1656720
  • 负责人:
  • 金额:
    $ 50.54万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-07-01 至 2021-06-30
  • 项目状态:
    已结题

项目摘要

Oysters and clams constitute not only shellfishery resources of high commercial value, but also very important components of the estuarine and coastal environment in the USA Therefore, infectious diseases in these bivalve species have serious impacts in both the shellfisheries industry and health of the environment. Among the several diseases that affect shellfish in the USA, Perkinsus (Dermo) parasites have caused severe mortalities in natural and farmed oyster populations and no effective preventive or therapeutic solutions have been developed so far. For most shellfish diseases, little is known about the mechanisms of parasite specificity for the host, infection, and transmission. During prior studies the investigators discovered that Perkinsus parasites subvert a host defense protein (galectin) to gain entry in the oyster cells and cause disease. Now they will elucidate how the eastern oyster and softshell clam galectins determine the host specificity of the different Perkinsus species, how they modulate the survival of the parasite in oyster and clams, and how they negatively affect the functions of the immune cells. This information will be critical to understand how and why the different Perkinsus species are more or less pathogenic for certain shellfish species. This fundamental knowledge will enable the development of innovative methods to prevent infections, pathogenic effects of the parasites, and their transmission to healthy shellfish populations. Educational and training aspects will be actively pursued specifically incorporating underrepresented minorities and women. The fundamental knowledge obtained will contribute new insight into parasitic disease in animals of agricultural interest and man.In contrast with Perkinsus marinus that is highly pathogenic for eastern oysters, the sympatric P. chesapeaki, initially isolated from the softshell clam Mya arenaria, can also be present in oysters but there is little evidence of pathogenicity. Prior studies revealed in the eastern oyster two opsonic galectins that strongly recognize P. marinus, but weakly recognize P. chesapeaki; the clam expresses a galectin of distinct specificity. This led to the hypothesis that host preference and pathogenicity of Perkinsus species is directly related to galectin-mediated host entry, and ability to modulate hemocyte function and expression of galectins upon infection. This hypothesis will be tested by using molecular, genetic, biochemical, structural and cell biology approaches to elucidate: (a) the structural basis for differential recognition of Perkinsus spp by the oyster and clam galectins; (b) the effects of galectin-mediated differential recognition of Perkinsus spp on opsonization, phagocytosis, and intrahemocytic parasite survival; and (c) the mechanisms mediated by galectin binding to parasite and hemocyte surface receptors and co-receptors and the modulation of signaling pathways that regulate hemocyte function(s). Thus, the proposed activity will be highly transformative of the long-held concept that parasite mimicry is a strategy to avoid recognition by the host, while the proposed model would reveal acquisition of glycomimicry by the parasite aimed not only at promoting host recognition for entry and pathogenicity, but with necessary specificity to determine host preference.
牡蛎和文蛤是美国具有较高经济价值的贝类资源,也是河口和海岸环境的重要组成部分。因此,牡蛎和文蛤的传染病对贝类养殖业和环境健康具有严重的影响。在影响美国贝类的几种疾病中,Perkinsus(Dermo)寄生虫已导致天然和养殖牡蛎种群的严重死亡,迄今为止尚未开发出有效的预防或治疗方案。对于大多数贝类疾病,很少有人知道寄生虫对宿主的特异性,感染和传播的机制。在之前的研究中,研究人员发现帕金斯寄生虫破坏宿主防御蛋白(半乳糖凝集素)进入牡蛎细胞并引起疾病。现在,他们将阐明东部牡蛎和软壳蛤半乳糖凝集素如何决定不同帕金虫物种的宿主特异性,它们如何调节牡蛎和蛤中寄生虫的生存,以及它们如何对免疫细胞的功能产生负面影响。这些信息对于理解不同的帕金苏斯物种如何以及为什么对某些贝类物种或多或少具有致病性至关重要。这一基本知识将使创新方法的发展,以防止感染,寄生虫的致病作用,及其传播到健康的贝类种群。将积极开展教育和培训方面的工作,特别是吸收代表性不足的少数民族和妇女参加。所获得的基本知识将有助于新的见解寄生虫病的动物的农业利益和人。与帕金斯marinus是高致病性的东方牡蛎,同域P. chesapeaki,最初从软壳蛤Mya arenaria分离,也可以存在于牡蛎,但几乎没有证据表明致病性。先前的研究显示,在东部牡蛎两个调理半乳糖凝集素,强烈识别P. marinus,但弱识别P. chesapeaki;蛤表达的半乳糖凝集素的独特的特异性。这导致了这样的假设,即帕金虫属物种的宿主偏好和致病性与半乳糖凝集素介导的宿主进入以及在感染后调节血细胞功能和半乳糖凝集素表达的能力直接相关。这一假设将通过使用分子、遗传、生物化学、结构和细胞生物学方法来验证,以阐明:(a)牡蛎和蛤半乳糖凝集素对帕金虫属的差异识别的结构基础;(B)半乳糖凝集素介导的帕金虫属的差异识别对调理作用、吞噬作用和血细胞内寄生虫存活的影响;和(c)半乳糖凝集素与寄生虫和血细胞表面受体和辅助受体结合所介导的机制,以及调节血细胞功能的信号传导途径的调节。因此,拟议的活动将是高度变革的长期持有的概念,寄生虫模仿是一种策略,以避免识别的主机,而拟议的模型将揭示收购glycomimicry的寄生虫,目的不仅是促进主机识别进入和致病性,但必要的特异性,以确定主机的偏好。

项目成果

期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Biochemical Characterization of Oyster and Clam Galectins: Selective Recognition of Carbohydrate Ligands on Host Hemocytes and Perkinsus Parasites
牡蛎和蛤半乳糖凝集素的生化表征:宿主血细胞和帕金瑟斯寄生虫上碳水化合物配体的选择性识别
  • DOI:
    10.3389/fchem.2020.00098
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    5.5
  • 作者:
    Vasta, Gerardo R.;Feng, Chiguang;Tasumi, Satoshi;Abernathy, Kelsey;Bianchet, Mario A.;Wilson, Iain B.;Paschinger, Katharina;Wang, Lai-Xi;Iqbal, Muddasar;Ghosh, Anita
  • 通讯作者:
    Ghosh, Anita
Introduction to special issue: Pattern recognition receptors and their roles in immunity in invertebrates
特刊简介:模式识别受体及其在无脊椎动物免疫中的作用
Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand
  • DOI:
    10.1093/glycob/cwaa034
  • 发表时间:
    2020-11-01
  • 期刊:
  • 影响因子:
    4.3
  • 作者:
    Mendoza, Mirian;Lu, Dongli;Dveksler, Gabriela
  • 通讯作者:
    Dveksler, Gabriela
Structure of the zebrafish galectin-1-L2 and model of its interaction with the infectious hematopoietic necrosis virus (IHNV) envelope glycoprotein
  • DOI:
    10.1093/glycob/cwz015
  • 发表时间:
    2019-05-01
  • 期刊:
  • 影响因子:
    4.3
  • 作者:
    Ghosh, Anita;Banerjee, Aditi;Bianchet, Mario A.
  • 通讯作者:
    Bianchet, Mario A.
Lacking catalase, a protistan parasite draws on its photosynthetic ancestry to complete an antioxidant repertoire with ascorbate peroxidase
  • DOI:
    10.1186/s12862-019-1465-5
  • 发表时间:
    2019-07
  • 期刊:
  • 影响因子:
    3.4
  • 作者:
    E. Schott;E. Schott;S. D. Lella;T. Bachvaroff;L. Amzel;G. Vasta
  • 通讯作者:
    E. Schott;E. Schott;S. D. Lella;T. Bachvaroff;L. Amzel;G. Vasta
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Gerardo Vasta其他文献

Classification and identification of Pfiesteria and Pfiesteria-like species.
Pfiesteria 和 Pfiesteria 类物种的分类和鉴定。
  • DOI:
    10.2307/3454911
  • 发表时间:
    2001
  • 期刊:
  • 影响因子:
    10.4
  • 作者:
    K. Steidinger;Jan H. Landsberg;R. William Richardson;Earnest W. Truby;Barbara Blakesley;Paula Scott;Patricia Tester;Torstein Tengs;Patrice Mason;Steve L. Morton;David W. Seaborn;Wayne Litaker;Kimberly Reece;David Oldach;Leonard W. Haas;Gerardo Vasta
  • 通讯作者:
    Gerardo Vasta

Gerardo Vasta的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Gerardo Vasta', 18)}}的其他基金

Roles of galectins in viral infection of mucosal epithelia using the zebrafish model system
使用斑马鱼模型系统研究半乳糖凝集素在粘膜上皮病毒感染中的作用
  • 批准号:
    2235553
  • 财政年份:
    2023
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Continuing Grant
Collaborative Research: Host-Pathogen Interactions at Pallial Interfaces in Marine Bivalves: Cellular and Molecular Pathways for Host Colonization and Invasion
合作研究:海洋双壳类动物大脑皮层界面的宿主-病原体相互作用:宿主定植和入侵的细胞和分子途径
  • 批准号:
    1050518
  • 财政年份:
    2011
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Standard Grant
A novel Galectin type as a Surface Receptor for Intracellular Parasites
一种新型半乳糖凝集素作为细胞内寄生虫的表面受体
  • 批准号:
    1063729
  • 财政年份:
    2010
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Standard Grant
The Role(s) of the Cation Transporter Nramp in the Intracellular Survival of Protistan Parasites
阳离子转运蛋白 Nramp 在原生生物寄生虫细胞内生存中的作用
  • 批准号:
    0958016
  • 财政年份:
    2010
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Continuing Grant
A Novel Galectin Type as a Surface Receptor for Intracellular Parasites
一种新型半乳糖凝集素作为细胞内寄生虫的表面受体
  • 批准号:
    0822257
  • 财政年份:
    2008
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Continuing Grant
Organellar targeting, regulation, and biological role(s) of antioxidant enzymes in the protistan parasite Perkinsus marinus
原生动物寄生虫 Perkinsus marinus 中抗氧化酶的细胞器靶向、调节和生物学作用
  • 批准号:
    0618409
  • 财政年份:
    2006
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Standard Grant
Collaborative Research: Origins and Spread of the Aspergillus- Gorgonian Coral Epizootic: Role of Climate and Environmental Facilitators
合作研究:曲霉-柳珊瑚珊瑚流行病的起源和传播:气候和环境促进因素的作用
  • 批准号:
    0326698
  • 财政年份:
    2003
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Standard Grant
Intracellular Survival of Protistan Parasites: Role(s) of the Host and Parasite Membrane Transporter Slc11a in the Competition for Iron
原生生物寄生虫的细胞内存活:宿主和寄生虫膜转运蛋白 Slc11a 在铁竞争中的作用
  • 批准号:
    0321417
  • 财政年份:
    2003
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Standard Grant
Tunicate Lectins as Acute Phase Reactants in Innate Immunity
被囊类凝集素作为先天免疫中的急性期反应物
  • 批准号:
    0077928
  • 财政年份:
    2000
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Continuing Grant
Tunicate C-type Lectins as Acute Phase Reactants
被囊动物 C 型凝集素作为急性期反应物
  • 批准号:
    9406649
  • 财政年份:
    1994
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Continuing grant

相似国自然基金

疣螈属动物的分子系统地理学研究
  • 批准号:
    30870281
  • 批准年份:
    2008
  • 资助金额:
    32.0 万元
  • 项目类别:
    面上项目

相似海外基金

Excellence in Research: Microbiome of the Eastern Oyster (Crassostrea virginica) and its denitrification potential in benthic systems
卓越研究:东部牡蛎(Crassostrea virginica)的微生物组及其在底栖系统中的反硝化潜力
  • 批准号:
    1901371
  • 财政年份:
    2019
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Standard Grant
Temporal Resolution of Haplosporidium nelsoni infection of Eastern Oyster (Crassostrea virginica)
东部牡蛎(Crassostrea virginica)尼尔氏单孢子虫感染的时间消退
  • 批准号:
    538519-2019
  • 财政年份:
    2019
  • 资助金额:
    $ 50.54万
  • 项目类别:
    University Undergraduate Student Research Awards
Excellence in Research: Microbiome of the Eastern Oyster (Crassostrea virginica) and its denitrification potential in benthic systems
卓越研究:东部牡蛎(Crassostrea virginica)的微生物组及其在底栖系统中的反硝化潜力
  • 批准号:
    1901377
  • 财政年份:
    2019
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Standard Grant
NSF Postdoctoral Fellowship in Biology FY 2017: A large-scale, experimental test of local adaption in the eastern oyster along a natural salinity gradient in the Gulf of Mexico
2017 财年 NSF 生物学博士后奖学金:对墨西哥湾东部牡蛎沿自然盐度梯度的局部适应进行大规模实验测试
  • 批准号:
    1711319
  • 财政年份:
    2017
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Fellowship Award
Documenting genetic diversity of wild Eastern Oyster populations towards improving production
记录野生东部牡蛎种群的遗传多样性以提高产量
  • 批准号:
    517848-2017
  • 财政年份:
    2017
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Engage Plus Grants Program
Determination of Environmental MSX Vectors for Eastern Oyster
东部牡蛎环境 MSX 载体的测定
  • 批准号:
    496684-2016
  • 财政年份:
    2016
  • 资助金额:
    $ 50.54万
  • 项目类别:
    University Undergraduate Student Research Awards
Population genomics towards improving Eastern Oyster production
群体基因组学提高东部牡蛎产量
  • 批准号:
    505758-2016
  • 财政年份:
    2016
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Engage Grants Program
Establishment of a commercial breeding program for the Eastern oyster, crassostrea virginica
建立东部牡蛎(crassostrea virginica)商业育种计划
  • 批准号:
    451413-2013
  • 财政年份:
    2015
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Industrial R&D Fellowships (IRDF)
Establishment of a commercial breeding program for the Eastern oyster, crassostrea virginica
建立东部牡蛎(crassostrea virginica)商业育种计划
  • 批准号:
    451413-2013
  • 财政年份:
    2014
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Industrial R&D Fellowships (IRDF)
Establishment of a commercial breeding program for the Eastern oyster, crassostrea virginica
建立东部牡蛎(crassostrea virginica)商业育种计划
  • 批准号:
    451413-2013
  • 财政年份:
    2013
  • 资助金额:
    $ 50.54万
  • 项目类别:
    Industrial R&D Fellowships (IRDF)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了