Identification and function of regulatory RNA in the phototrophic model organism Synechocystis sp. PCC 6803

来自原核生物和真核生物的最新数据显示，非编码RNA(NcRNAs)作为基因表达的序列特异性调节因子具有极高的潜力，从而介导了大量细胞对变化的环境线索和形态分化的反应。在这里，我们建议通过克隆和测序蓝藻聚球藻的所有小RNA来全面和系统地解开全套ncRNAs。PCC6803，与基因间隔区ncRNA基因的计算预测相平行。聚球藻。PCC6803是基因上研究得最好、最容易处理的蓝藻。因此，我们计划利用这些信息，通过定向敲除编码最有希望的ncRNA基因和在RNA代谢中具有关键功能的蛋白质(RNase IM、RNase E、Hfq)的基因的敲除突变，将这种生物发展成为分析蓝藻调控RNA的真实模型。我们将特别重视对一个ncRNA的功能分析，我们在导致这一建议的先前工作过程中确定了这一点。该ncRNA全长为ISIA编码区的反义方向。在铁限制和氧化还原胁迫下，ISIA的转录水平急剧增加，这种ncRNA参与了这种增加的控制。

Recent data from pro- and eukaryotic organisms show the extremely high potential of non-coding RNAs (ncRNAs) as sequence-specific regulators of gene expression, thereby mediating a plethora of cellular responses to changing environmental clues, and morphological differentiation. Here, we suggest to unravel the full set of ncRNAs in a comprehensive and systematical way by cloning and sequencing all small RNAs from the cyanobacterium Synechocystis sp. PCC 6803, paralleled by a computational prediction of ncRNA genes in intergenic regions. Synechocystis sp. PCC 6803 is the genetically best studied and easiest to handle cyanobacterium. We therefore plan to use this information to develop this organism into a true model for the analysis of regulatory RNAs in cyanobacteria by targeted knock-out mutations of genes encoding the most promising ncRNA genes and proteins with key functions in the metabolism of RNA (RNase IM, RNase E, Hfq). We will put special emphasis on the functional analysis of one ncRNA, which we have identified in the course of prior work that has led to this proposal. This ncRNA is over its full length in antisense orientation to the coding region of isiA. Transcript levels for isiA increase sharply under iron limitation and redox stress and this ncRNA is involved in the control of this increase.