Identification and function of regulatory RNA in the phototropic model organism Synechocystis sp. PCC 6803

向光性模式生物集胞藻中调节RNA的鉴定和功能。

• 批准号: 41591666
• 负责人: Professor Dr. Wolfgang R. Hess
• 金额: --
• 依托单位: Institut für Biologie III
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/41591666?language=en
• 关键词: Identification; function; regulatory; RNA; phototropic

Non-coding RNAs (ncRNAs) serve as sequence-specific regulators of gene expression in all domains of life. In the previous funding period we have unraveled the nearly complete set of ncRNAs and antisense RNAs in the model cyanobacterium Synechocystis sp. PCC 6803 in a comprehensive and systematical way using a combination of transcriptome sequencing, detailed microarray analysis, computational prediction of ncRNA genes, complemented by extensive Northern and 5’ TAP-RACE experiments. These analyses demonstrated the presence of several hundred such loci giving rise to potentially regulatory RNA molecules. We performed knock-out mutations of genes for several of the most promising ncRNA genes and proteins with key functions in the metabolism of RNA (RNase III, RNase E/G, Hfq), developing Synechocystis sp. PCC 6803 into a true model for the analysis of regulatory RNAs in cyanobacteria. In the new funding period we will shift our focus on the functional analysis of several selected ncRNAs, plasmid-encoded CRISPR-RNAs, the cyanobacterial Hfq homologue and RNA degradation pathways.

非编码RNA（ncRNA）在生命的所有领域中充当基因表达的序列特异性调节剂。在前一个资助期间，我们已经以全面和系统的方式，使用转录组测序、详细的微阵列分析、ncRNA基因的计算预测的组合，辅以广泛的北方和5' TAP-RACE实验，解开了模型蓝细菌集胞藻属PCC 6803中几乎完整的ncRNA和反义RNA组。这些分析表明，存在几百个这样的基因座，产生潜在的调节RNA分子。我们对几个最有前途的ncRNA基因和在RNA代谢中具有关键功能的蛋白质（RNase III，RNase E/G，Hfq）进行基因敲除突变，将集胞藻PCC 6803发展成用于分析蓝藻中调控RNA的真正模型。在新的资助期内，我们将把重点转移到几种选定的ncRNA、质粒编码的CRISPR-RNA、蓝藻Hfq同源物和RNA降解途径的功能分析上。