Novel Cyanobacterial Toxin-Antitoxin Systems

新型蓝藻毒素-抗毒素系统

基本信息

项目摘要

Bacterial toxin-antitoxin (TA) systems are known as genetic elements, which are encoded by plasmid as well as chromosomal loci and mediate plasmid and genomic island maintenance through post-segregational killing mechanisms. However, TA systems exist in surprisingly high numbers in all pro-karyotes and a growing number of publications suggest TA systems with milder effects to act as mobile stress response systems which help certain cells of a population in persisting adverse growth conditions. TA systems can trigger cell cycle arrest as well as programmed cell death; accordingly, DNA replication and metabolism, translation and cell membrane organization are among their verified cellular targets. In cyanobacteria, oxygenic photosynthesis and thylakoid membranes appear as at-tractive additional targets, but not a single cyanobacterial TA system has been experimentally charac-terized so far. This is in contrast to the results of our own initial analyses, which point to a high number of TA cassettes controlled by peptide antitoxins or antisense RNA. This proposal aims at the molecular characterization of seven putative cyanobacterial TA systems. Among our objectives is the characterization of their control, regulation and mode of action, to understand how cells sense and cope with these factors and how the respective developmental and stress response programs are triggered. The results will have implications for the application of TA systems as natural resistance markers in biotechnology, the development of novel substances with antibiotic properties and are fundamentally relevant to understand how bacteria can survive adverse conditions.
细菌毒素-抗毒素(TA)系统被称为遗传元件,由质粒和染色体位点编码,并通过分离后杀伤机制介导质粒和基因组岛的维持。然而,在所有原核生物中,TA系统的数量惊人地多,越来越多的出版物表明,TA系统具有较温和的作用,可作为移动应激反应系统,帮助群体中的某些细胞持续不利的生长条件。TA系统可以触发细胞周期阻滞和程序性细胞死亡;因此,DNA的复制和代谢、翻译和细胞膜组织是它们证实的细胞靶标。在蓝藻中,含氧光合作用和类囊体膜似乎是吸引的额外目标,但迄今为止还没有一个单一的蓝藻TA系统被实验表征。这与我们自己的初步分析结果相反,后者指出大量的TA磁带是由肽抗毒素或反义RNA控制的。本提案旨在分子表征七个假定的蓝藻TA系统。我们的目标之一是表征它们的控制、调节和作用模式,以了解细胞如何感知和应对这些因素,以及如何触发各自的发育和应激反应程序。这些结果将对TA系统在生物技术中作为天然抗性标记的应用、具有抗生素特性的新物质的开发以及了解细菌如何在不利条件下生存的根本相关意义产生影响。

项目成果

期刊论文数量(1)
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Professor Dr. Wolfgang R. Hess其他文献

Professor Dr. Wolfgang R. Hess的其他文献

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{{ truncateString('Professor Dr. Wolfgang R. Hess', 18)}}的其他基金

CRISPR/Cas systems in cyanobacteria, their involvement in cell differentiation and potential for metabolic manipulation
蓝藻中的 CRISPR/Cas 系统、它们对细胞分化的参与以及代谢操纵的潜力
  • 批准号:
    391592464
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Hydrocarbon production in genetically engineered cyanobacteria
基因工程蓝细菌中的碳氢化合物生产
  • 批准号:
    242480706
  • 财政年份:
    2013
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Analysis of CRISPR-Cas systems in Cyanobacteria
蓝藻中 CRISPR-Cas 系统的分析
  • 批准号:
    206949509
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Research Units
Identification and function of regulatory RNA in the phototropic model organism Synechocystis sp. PCC 6803
向光性模式生物集胞藻中调节RNA的鉴定和功能。
  • 批准号:
    41591666
  • 财政年份:
    2007
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Identification and function of regulatory RNA in the phototrophic model organism Synechocystis sp. PCC 6803
光养模式生物集胞藻中调节RNA的鉴定和功能。
  • 批准号:
    22770769
  • 财政年份:
    2006
  • 资助金额:
    --
  • 项目类别:
    Research Grants
The role of small proteins, peptides and dual function transcripts in cyanobacteria
小蛋白、肽和双功能转录物在蓝藻中的作用
  • 批准号:
    379644176
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Evolution of the regulatory networks governing developmental adaptations and acclimation responses in Charophycean green algae towards plant life on land
轮藻类绿藻对陆地植物生命的发育适应和驯化反应的调控网络的演变
  • 批准号:
    440274755
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Cross-talk and extensive rewiring of CRISPR-Cas systems with the cellular regulatory machinery in cyanobacteria
CRISPR-Cas系统与蓝藻细胞调节机制的串扰和广泛重连
  • 批准号:
    405822239
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
An integrated investigation of regulatory RNAs and their interacting proteins in bacteria models
细菌模型中调节 RNA 及其相互作用蛋白的综合研究
  • 批准号:
    530000184
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Novel regulators and interactions within the regulatory networks controlling the autotrophy -heterotrophy switch in cyanobacterial carbon metabolism
控制蓝藻碳代谢中自养-异养转换的新型调节因子和调节网络内的相互作用
  • 批准号:
    415276064
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Research Units

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Horizontal gene transfer of cyanobacterial carbon fixing machinery: Implications for the rise of modern atmospheric oxygen
蓝藻固碳机制的水平基因转移:对现代大气氧气上升的影响
  • 批准号:
    NE/Z00019X/1
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Shifting paradigms to emerging toxins in freshwater cyanobacterial blooms
淡水蓝藻水华中新出现的毒素的范式转变
  • 批准号:
    10912318
  • 财政年份:
    2023
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Cyanobacterial extracellular metabolomes: overlooked drivers of ecosystem ecology and evolution
蓝藻细胞外代谢组:被忽视的生态系统生态和进化驱动因素
  • 批准号:
    23K19391
  • 财政年份:
    2023
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    --
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    Grant-in-Aid for Research Activity Start-up
Live-imaging analysis to reveal spatiotemporal dynamics of cyanobacterial light signaling
实时成像分析揭示蓝藻光信号的时空动态
  • 批准号:
    22KJ1374
  • 财政年份:
    2023
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    --
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    Grant-in-Aid for JSPS Fellows
CAREER: Species Interactions of Coral Reef Benthic Cyanobacterial Mats: Within-Mat Diversity Promotes Both Facilitation and Top-Down Control
职业:珊瑚礁底栖蓝藻垫的物种相互作用:垫内多样性促进便利化和自上而下的控制
  • 批准号:
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    2023
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Collaborative Research: ORCC: Carbon fixation in future oceans: experimental adaptation of algal and cyanobacterial CO2-concentrating mechanisms to a changing climate
合作研究:ORCC:未来海洋的碳固定:藻类和蓝藻二氧化碳浓缩机制对气候变化的实验适应
  • 批准号:
    2222519
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    2023
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    Standard Grant
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合作研究:ORCC:未来海洋的碳固定:藻类和蓝藻二氧化碳浓缩机制对气候变化的实验适应
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URoL:EN: Understanding the rule of life facilitating the proliferation of toxic cyanobacterial benthic mats in flowing freshwaters
URoL:EN:了解促进有毒蓝藻底栖垫在流动淡水中增殖的生命规则
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    2222322
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    2023
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Enhancing and Redirecting Cyanobacterial Electron Flow (Bioelectricity Spotlight)
增强和重定向蓝藻电子流(生物电聚光灯)
  • 批准号:
    BB/Y001370/1
  • 财政年份:
    2023
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14th Workshop on Cyanobacteria: Promoting collaborative science and early career scientists in the field of cyanobacterial physiology and applications
第14届蓝藻研讨会:促进蓝藻生理学和应用领域的合作科学和早期职业科学家
  • 批准号:
    2221007
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    2022
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