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Factors determining vector competence of the thrips Frankliniella occidentalis for transmitting TSWV Tospovirus

蓟马西花蓟马传播 TSWV Tospovirus 的媒介能力决定因素

基本信息

批准号：
228154671
负责人：
Professor Dr. Hans-Michael Poehling
金额：
--
依托单位：
Abteilung Phytomedizin
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/228154671?language=en
关键词：
Factors determining vector competence thrips

项目摘要

The epidemiology of the economically important Tomato Spotted Wild Virus (TSWV) is determined by the vector competence of thrips species such as Frankliniella occidentalis. The main aim of the study is to determine factors responsible for the high variability in vector competence of F. occidentalis individuals and populations. The study will be divided in three parts: (1) Investigation of the heritability of the trait vector competence of F. occidentalis for transmitting the TSWV tospovirus on sweet pepper (Capsicum annum) as a model crop. This part consists of crossings with individually characterized vector competent and non-competent females and males of F. occidentalis (single pair crossing). Crossing schemes include back-crossings (F1 -- F2). Frequency of competent individuals (phenotype) will be determined in a biotest, moreover competent vs non-competent individuals should be characterized by genetic markers such as microsatellites and/ or AFLPs to determine allele frequencies on the genome level (genotype) in addition. (2) Determination of the dynamics (over generations) of vector competence (percentage of competent individuals) in differently structured populations over multiple generations in isolated populations of different sizes, of different initial proportions of competent and non-competent individuals and after artificial fragmentation of populations (gene drift and bottle-neck effects). (3) The third part will focus on selected non-genetic factors assumed to additionally influence vector competence or allele shifts in isolated populations. Parameters to be evaluated from individuals characterized as either competent or non-competent will be mating preferences, host plant selection, feeding behavior and mobility (host plant switch) as well as fitness parameters such as longevity and fecundity.

番茄斑点野生病毒（Tomato Spotted Wild Virus，TSWV）是一种具有重要经济意义的病毒，其流行病学主要由蓟马（Frankliniella ococcuentalis）等蓟马的传毒能力决定。本研究的主要目的是确定导致F. Occupientalis个体和种群。本研究分为三个部分：（1）F.在作为模式作物的甜椒（Capsicum annum）上传播TSWV番茄斑萎病毒。这部分包括与个别特点的载体主管和非主管女性和男性的F。单对杂交（single pair crossing）。交叉方案包括回交（F1 - F2）。将在生物测试中确定有能力个体（表型）的频率，此外，应通过遗传标记（如微卫星和/或AFLP）来表征有能力与无能力个体，以确定基因组水平（基因型）上的等位基因频率。 (2)在不同规模、不同初始比例的有能力和无能力个体的隔离群体中，以及在人为的群体碎片化（基因漂移和瓶颈效应）之后，确定不同结构群体中病媒能力（有能力个体的百分比）在多代中的动态（世代）。(3)第三部分将集中在选定的非遗传因素，假设另外影响载体的能力或等位基因的变化，在孤立的人口。从个体中评价的参数，无论是有能力的还是没有能力的，将是交配偏好、寄主植物选择、摄食行为和移动性（寄主植物转换）以及适合度参数，如寿命和繁殖力。