Deciphering the role of comammox bacteria in nitrogen removal systems

破译comammox细菌在脱氮系统中的作用

• 批准号: 1703089
• 负责人: Ameet Pinto
• 金额: $ 33万
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1703089&HistoricalAwards=false
• 关键词: Deciphering; role; comammox; bacteria; nitrogen

Nitrification is a chemical reaction process that plays an important role in the removal of organic nitrogen from municipal wastewater. Nitrification is also an energy intensive process. The recent discovery of a single organism (the comammox bacterium: COMplete AMMonia OXidiser) that can perform nitrification, has led to research into whether this bacterium may be useful for creating a less energy intensive process for nitrogen removal from wastewater. The ability to manage comammox impact on nitrogen removal is critically dependent on a fundamental understanding of its ecology, physiology, and engineering relevance. This research project advances our understanding of the comammox bacterium by developing novel, scalable, and quantitative methods for its characterization. The project goals are being accomplished by coupling state-of-the-art molecular, bioinformatics, and statistical methods with laboratory and field experiments. This project is yielding fundamental insights into comammox bacterial importance, ecology, and physiology and informing process strategies to exploit this novel organism for sustainable nitrogen removal in wastewater treatment plants. The research plan is integrated with a comprehensive suite of educational outreach activities involving undergraduate, graduate, and K-12 students. The activities include a blend of bioinformatics training for undergraduate and graduate students, mentoring of researchers from underrepresented minority groups, and modules for K-12 students on wastewater treatment.Ensuring the effectiveness and sustainability of nitrogen removal from wastewater requires a detailed understanding of its biotransformation pathways. The recent discovery of comammox bacterium represents a paradigm shift in the understanding of aerobic nitrification, the first key step in nitrogen removal. The research team is investigating the ecology, physiology, and engineering relevance of the comammox bacterium in four key nitrogen removal process configurations. A primary objective of the project is to develop novel methods to characterize the abundance, diversity, and activity of comammox bacteria in complex microbial communities. A deeper understanding of the impact of process and environmental conditions on comammox bacteria will lead to improved strategies to manage and manipulate their contribution in nitrogen removal. Results of this project are advancing our understanding of the role and relevance of novel comammox bacteria within complex nitrifying communities.

硝化作用是一种化学反应过程，在城市污水中有机氮的去除中起着重要作用。 硝化作用也是一种能量密集型过程。 最近发现了一种可以进行硝化的单一生物体（comammox细菌：COMPLETE AMMonia OXidiser），这使得人们开始研究这种细菌是否可以用于创造一种能量密集度较低的废水脱氮过程。 管理comammox对氮去除的影响的能力关键取决于对其生态学，生理学和工程相关性的基本理解。该研究项目通过开发新的、可扩展的和定量的方法来表征comammox细菌，从而促进了我们对comammox细菌的理解。 该项目的目标正在通过耦合国家的最先进的分子，生物信息学和统计方法与实验室和现场实验。该项目正在产生对comammox细菌重要性，生态学和生理学的基本见解，并为利用这种新型生物体在污水处理厂中可持续脱氮提供工艺策略。该研究计划与涉及本科生，研究生和K-12学生的教育推广活动的综合套件相结合。这些活动包括为本科生和研究生提供生物信息学培训，指导来自代表性不足的少数群体的研究人员，以及为K-12学生提供废水处理模块。确保废水脱氮的有效性和可持续性需要详细了解其生物转化途径。最近发现的comammox细菌代表了一个范式转变的理解好氧硝化，脱氮的第一个关键步骤。该研究小组正在调查comammox细菌在四个关键脱氮工艺配置中的生态学，生理学和工程相关性。该项目的主要目标是开发新的方法来表征复杂微生物群落中comammox细菌的丰度，多样性和活性。更深入地了解工艺和环境条件对comammox细菌的影响，将导致改进的策略来管理和操纵它们在脱氮中的贡献。这个项目的结果推进了我们对复杂硝化群落中新型comammox细菌的作用和相关性的理解。

项目成果

Long solids retention times and attached growth phase favor prevalence of comammox bacteria in nitrogen removal systems

• DOI: 10.1016/j.watres.2019.115268
• 发表时间: 2020-02-01
• 期刊: WATER RESEARCH
• 影响因子: 12.8
• 作者: Cotto, Irmarie;Dai, Zihan;Pinto, Ameet J.
• 通讯作者: Pinto, Ameet J.