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Microfabricated devices for flexible sensing at the biointerface

用于生物界面灵活传感的微加工设备

基本信息

批准号：
1704435
负责人：
Vamsi Yadavalli
金额：
$ 30万
依托单位：
Virginia Commonwealth University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2021-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1704435&HistoricalAwards=false
关键词：
Microfabricated devices flexible sensing biointerface

项目摘要

Sensors that are biocompatible and biodegradable and are mechanically flexible and conform to soft tissues and organs in the human body are highly desirable as they provide a means to measure and monitor activity. In this research, biodegradable silk will be engineered and fabricated for testing of measurement feasibility of biomarkers that are indicative of pathology. The project will investigate various electrochemical biosensor designs (1D wires, 2D sheets and 3D blocks) using biodegradable silk and conducting polymers in flexible and conformable formats for measuring such model biomarkers as acetylcholine, C-reactive protein, vascular endothelial growth factor (VEGF). The innovation proposed is the method of fabricating functional biosensors comprising the conducting polymer poly (3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) and silk proteins (fibroin and sericin) via techniques developed by silk protein lithography. The structure-property relationships of fabricated biosensors will be investigated through the following specific aims. (1) Investigate the compositional relationships that enhance electrochemical response and mechanical properties, and enable their selective detection, and (2) Investigate sensor fabrication strategies for sensors in multi-scales and dimensions (1-D wires, 2D sheets, and 3D scaffold network) so that they can conform to soft tissue. Appropriate recognition elements (antibody and aptamers) will be immobilized on electrode surface against the target analyte. Sensor response and mechanical properties will be engineered for conformable attachment to the target soft tissue while minimizing cytotoxicity. Feasibility sensor experiments will be conducted in vitro using stock seeds of cells under physiological conditions. Reproducibility of sensor response will be characterized and sensor design will be modified to accommodate required sensitivity, selectivity, and reproducibility. Education and outreach activities are proposed. Haptic and 3D printed models of micro and nanoscale objects will be fabricated to give students a direct visualization and textural feel for the various kinds of sensor architectures. Sharing of these in annual outreach activities organized by the PI will help inspire young students into engineering and science careers.

高度期望生物相容性和生物可降解性并且机械柔性且符合人体中的软组织和器官的传感器，因为它们提供测量和监测活动的手段。在这项研究中，将设计和制造可生物降解的丝，用于测试指示病理学的生物标志物的测量可行性。该项目将研究各种电化学生物传感器设计（1D线，2D片和3D块），使用可生物降解的丝和导电聚合物以灵活和一致的形式测量乙酰胆碱，C反应蛋白，血管内皮生长因子（VEGF）等模型生物标志物。所提出的创新是制造功能性生物传感器的方法，所述功能性生物传感器包括导电聚合物聚（3，4-亚乙基二氧噻吩）：聚（苯乙烯磺酸盐）（PEDOT：PSS）和丝蛋白（丝素蛋白和丝胶蛋白），通过由丝蛋白光刻技术开发的技术。通过以下具体目标，将研究制造的生物传感器的结构-性能关系。 (1)研究增强电化学响应和机械性能的组成关系，并使其能够选择性检测，以及（2）研究多尺度和多维度（1-D线，2D片和3D支架网络）传感器的传感器制造策略，以便它们可以符合软组织。适当的识别元件（抗体和适体）将被固定在电极表面上以对抗目标分析物。传感器响应和机械性能将被设计为与目标软组织贴合，同时最大限度地减少细胞毒性。可行性传感器实验将在体外使用生理条件下的细胞储备种子进行。将对传感器响应的再现性进行表征，并修改传感器设计，以适应所需的灵敏度、选择性和再现性。建议开展教育和外联活动。将制作微纳米级物体的触觉和3D打印模型，为学生提供各种传感器架构的直接可视化和纹理感觉。在PI组织的年度外展活动中分享这些内容将有助于激励年轻学生从事工程和科学职业。