RUI:Development of a portable ECL immunosensor with mobile technologies

移动的技术正在成为未来生物传感器发展的中心。拟议的工作试图开发一种电化学发光（ECL）传感器利用现有的移动的（智能手机）技术，将传统上昂贵和笨重的生物传感器技术转变为便携式和负担得起的。ECL传感器的工作原理很简单-当一个小电压施加到ECL化学品上时，化学品就会发光。ECL与移动的技术相结合，可实现最小化的检测和移动的应用程序分析。 我们的目标是使这种新的传感器平台不仅在性能上等同于现有昂贵的生物传感器，而且更经济实惠，适合许多不同的生物传感器需求。本项目的目标是利用最新的移动的技术，改进传统的电化学发光（ECL）免疫传感器仪器，使其更加便携和廉价。当通过施加电势触发ECL反应时，发射的光处于可见光谱中，从而提供了满足超灵敏免疫测定和超紧凑形式的要求的巨大潜力。所提出的ECL生物传感器的成功开发将通过最大化移动的技术的实用性而显著增强生物传感器仪器，同时提供由昂贵和专用仪器实现的灵敏度。 已经尝试开发具有手机检测器的ECL传感器，但是它们在检测临床相关浓度的生物标志物方面没有成功。拟议工作的主要努力是将固有的低ECL信号增强到消费者相机可检测的水平，并开发最小的仪器用于有效的基于ECL的检测。 该研究将侧重于设计和开发与共反应物，丝网印刷电极上的微流体通道，触发反应的电路以及控制电路，成像和数据分析的移动的应用程序的协议。 ECL免疫传感器可用于疾病生物标志物的诊断。其廉价和便携的特性将使其成为灾难救援和恢复情况下使用的理想平台。 该项目将在一个本科为主的研究所进行，最大限度地提高教育方面，并强调社区外展。

Mobile technology is becoming the hub of future biosensor development. The proposed work attempts to develop an Electrochemiluminescence (ECL) sensor utilizing existing mobile (smartphone) technology, transforming what was traditionally an expensive and bulky biosensor technology into a portable and affordable one. ECL sensors work by a simple principle - when a small electric voltage is applied to an ECL chemical, the chemical emits light. The ECL combined with mobile technology enables minimal instrumentation and mobile app analysis. Our goal is to make this new sensor platform equivalent not only in performance to that of existing expensive biosensors, but also more affordable and for many different biosensor needs. The goal of this project is to advance the traditional electrochemiluminescence (ECL) immunosensor instrumentation to make it more portable and inexpensive using the latest mobile technologies. When an ECL reaction is triggered by the application of electric potential, the emitted light is in a visible spectrum, providing a great potential to satisfy the requirements for an ultra-sensitive immunoassay and in an ultra-compact format. Successful development of the proposed ECL biosensor will significantly enhance biosensor instrumentation by maximizing the practicality of mobile technologies while providing the sensitivity realized by expensive and specialized instruments. There have been attempts to develop ECL sensor with cell phone detectors but they have not been successful in detecting biomarkers at clinically relevant concentrations. The key effort of the proposed work are enhancing the inherently low ECL signal to a level that is detectable with consumer cameras and developing minimal instrumentation for efficient ECL-based detection. The research will focus on design as well as development of protocols with co-reactants, microfluidic channels on screen-printed-electrodes, electric circuits to trigger reactions, and a mobile app to control the circuit, imaging, and data analysis. The ECL immunosensor can be used for diagnosis of disease biomarkers. Its inexpensive and portable nature will make it an ideal platform to use in disaster rescue and recovery situations. The project will be performed at an undergraduate predominant institute maximizing educational aspects and emphasizing the community outreach.