Nanofluidic Charge Coupled Devices for Molecular Separation and Sensing

用于分子分离和传感的纳流体电荷耦合器件

• 批准号: 1710831
• 负责人: Weihua Guan
• 金额: $ 34.67万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1710831&HistoricalAwards=false
• 关键词: Nanofluidic; Charge; Coupled; Devices; Molecular

Molecular separation is at the heart of molecular diagnosis and analyzing molecular biomarkers in genomics and proteomics studies. Gel and capillary electrophoresis are two dominant molecule separation technologies based on charge/size dispersion. The research objective of this proposal is to explore a novel nanofluidic molecular charge-coupled device (CCD) suitable for integrated electro-fluidic molecular separation and sensing, fundamentally different from the conventional gel and capillary electrophoresis. The proposed nanofluidic molecular CCD is based on the unique field effect coupling in nanofluidic systems, in which new transport behavior and functionality can be developed by leveraging the unique electrostatic coupling at the nanometer. The success of the proposed project will transform the biomolecule sensing and separation by exploring the rich properties of the novel nanofluidic molecular CCD. The project also includes education and outreach activities to tightly integrate the research efforts and results with graduate, undergraduate, and K-12 education and to globally disseminate both research and the education outcomes.Molecule transport at nanometer scale plays an important role in many biological, chemical, physical and engineering systems. Nanoscale channels offer a unique platform to explore new phenomena appearing for molecule confined in nanometers scales. New transport behavior and functionalities can be developed by taking advantage of the specific couplings occurring at these scales. Despite the fact that CCD image sensors have been indispensable tools for a variety of applications in various scientific and engineering disciplines, a similar bucket brigade transport and analysis of charged biomolecules in nanochannels has not been explored and realized. The proposed research will explore the field effect controlled bucket brigade transport of charged molecules by rational design, simulation, fabrication, and validation of a novel nanofluidic molecule CCD device. The outcome of the research will enable a paradigm shift in controllable molecule transport and separation. The major challenges in realizing a CCD-like device for bucket brigade transport and analysis are the lack of fundamental understanding of the dynamic electric field coupling to the charged molecules at the nanoscale, and the lack of feasible top-down engineered fabrication methods to produce the metal-insulator-nanochannel (MIN) structure. The proposed research objectives will be achieved through the following three aims: (i) Understand the device physics of nanofluidic molecular CCD, (ii) Explore viable device fabrication and integration techniques, and (iii) Investigate nanofluidic molecular CCDs for biomolecule transport and separation.

分子分离是基因组学和蛋白质组学研究中分子诊断和分析分子生物标志物的核心。凝胶电泳和毛细管电泳是两种主要的基于电荷/尺寸分散的分子分离技术。本课题的研究目标是探索一种新型的纳米流控分子电荷耦合器件（CCD），它适用于集成化的电流体分子分离和传感，与传统的凝胶和毛细管电泳有着根本的区别。所提出的纳米流体分子CCD是基于纳米流体系统中独特的场效应耦合，其中可以通过利用纳米处独特的静电耦合来开发新的传输行为和功能。该项目的成功将通过探索新型纳米流体分子CCD的丰富特性来改变生物分子传感和分离。该项目还包括教育和推广活动，将研究工作和成果与研究生、本科生和K-12教育紧密结合，并在全球范围内传播研究和教育成果。纳米尺度的分子输运在许多生物、化学、物理和工程系统中起着重要作用。纳米尺度的通道为探索分子在纳米尺度下出现的新现象提供了一个独特的平台。新的传输行为和功能可以通过利用在这些尺度上发生的特定耦合来开发。尽管CCD图像传感器已经成为各种科学和工程学科中各种应用不可或缺的工具，但尚未探索和实现纳米通道中带电生物分子的类似水桶旅运输和分析。该研究将通过合理设计、模拟、制造和验证一种新型的纳米流体分子CCD器件来探索场效应控制的带电分子的桶旅传输。这项研究的结果将使可控分子运输和分离的范式转变成为可能。在实现一个类似CCD的设备的水桶旅运输和分析的主要挑战是缺乏基本的了解动态电场耦合到带电分子在纳米尺度上，并缺乏可行的自上而下的工程制造方法来生产的金属-绝缘体-纳米通道（MIN）结构。拟议的研究目标将通过以下三个目标来实现：（i）了解纳米流体分子CCD的器件物理，（ii）探索可行的器件制造和集成技术，以及（iii）研究用于生物分子传输和分离的纳米流体分子CCD。

项目成果

Microfluidic multiple cross-correlated Coulter counter for improved particle size analysis

• DOI: 10.1016/j.snb.2019.05.092
• 发表时间: 2019-10
• 期刊: Sensors and Actuators B: Chemical
• 作者: Wenchang Zhang;Yuan Hu;G. Choi;Shengfa Liang;Ming Liu;W. Guan

Microfluidic Time-Division Multiplexing Accessing Resistive Pulse Sensor for Particle Analysis

• DOI: 10.1021/acssensors.9b01067
• 发表时间: 2019-07-01
• 期刊: ACS SENSORS
• 影响因子: 8.9
• 作者: Choi, Gihoon;Murphy, Erica;Guan, Weihua
• 通讯作者: Guan, Weihua

Direct Observation of Redox-Induced Bubble Generation and Nanopore Formation Dynamics in Controlled Dielectric Breakdown

• DOI: 10.1021/acsaelm.0c00576
• 发表时间: 2020-09-22
• 期刊: ACS APPLIED ELECTRONIC MATERIALS
• 影响因子: 4.7
• 作者: Dong, Ming;Tang, Zifan;Guan, Weihua
• 通讯作者: Guan, Weihua

Non-centrifugal microfluidic nucleic acid testing on lab-on-a-disc

盘上实验室的非离心微流控核酸检测

• 发表时间: 2019
• 期刊: Transducer 2019
• 作者: Choi, Gihoon;Guan, Weihua
• 通讯作者: Guan, Weihua

False negative and false positive free nanopore fabrication via adaptive learning of the controlled dielectric breakdown

通过受控介电击穿的自适应学习实现无假阴性和假阳性的纳米孔制造

• 发表时间: 2019
• 期刊: Transducer 2019
• 作者: Rosha, Kamyar;Tang, Zifan;Guan, Weihua
• 通讯作者: Guan, Weihua