Immunity to Community: Can Quantifying Immune Traits Inform Reef Community Structure?

群落免疫：量化免疫特征能否为珊瑚礁群落结构提供信息？

• 批准号: 1712134
• 负责人: Laura Mydlarz
• 金额: $ 22.03万
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2020-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1712134&HistoricalAwards=false
• 关键词: Immunity; Community; Quantifying; Immune; Traits

Coral diseases have increased significantly throughout the past 30 years. Climate change and other detrimental environment factors are likely to blame. Unhealthy coral reefs cannot support the fish and other life that make the reef a vibrant and diverse ecosystem. Corals reefs in the Caribbean Sea are disease hotspots and many reefs have experienced population collapses due to outbreaks of disease. Importantly, coral species vary in their susceptible to disease, but the reasons behind this variation are unknown. This project will quantify coral susceptibility to disease by examining coral immunity using several novel approaches and experiments. Seven species of coral that differ in disease susceptibility, growth rates, growth form and reproductive strategies will be used. Immune responses of each species of coral will be measured by exposing the corals to bacterial immune stimulators. Susceptibility to white plague disease, a prevalent disease affecting many species of corals, will also be measured by exposing the corals to active white plague disease and calculating disease transmission rates. The immune response and disease transmission data for each coral species will be used to develop a predictive model to determine how different coral communities will respond to disease threats under climate change scenarios. This project will support graduate students at University of Texas, Arlington (Hispanic-serving Institution) and University of Virgin Islands (Historically Black University) and many undergraduate students at all three institutions (Mote Marine Laboratory). This research will be highlighted at outreach events at all three institutions which take place regularly and include Earth Day Texas in Dallas, TX, Mote's Living Reef Exhibit and Aquarium in Sarasota, FL and "Reef Fest" and Agricultural fairs in the U.S. Virgin Islands.Environmental changes, such as ocean warming, have led to an increase in the prevalence of coral diseases, causing region-wide population collapses in some locations. However, not all coral species, or even populations within species, are affected by disease equally. Some species are host to many different types of diseases, but have limited mortality. Other species suffer significant disease-related mortality. How and why disease susceptibility differs among species and the effects of this differential susceptibility on reef community structure and composition are currently unknown. This project will use immune-challenge experiments that will quantify novel components of the innate immune system of corals, coupled with the application of a trait-based model, to fulfill three goals: 1) Determine variability of coral immune traits in seven common coral species found on Caribbean reefs, 2) Determine the variability in resistance to white plague disease transmission in the same coral species 3) Develop a predictive model of coral community assemblage that incorporates immune traits. Quantification of coral immunity will also incorporate unique approaches, such as combining full transcriptome sequencing with protein activity assays for a gene-to-phenotype analysis. Data will be mapped onto immune pathways for comprehensive pathway evaluation between coral species and these will serve as trait inputs into a "traitspace" model. These traits will provide continuous data within the model, which will create a probability density function (PDF) for the trait distributions of each species. These PDFs will then be used to determine the probability of species under different disease exposure scenarios. Model analyses will determine which traits influence community structure and characterize how disease exposure and the immune response will predict community assemblages through space and time. The completion and application of a trait-base model that incorporates extensive immunity parameters (none of which have been applied to trait models within coral ecosystems) is a distinct product from this project.

在过去30年中，珊瑚疾病显著增加。气候变化和其他有害的环境因素可能是罪魁祸首。不健康的珊瑚礁无法支持鱼类和其他生命，使珊瑚礁成为一个充满活力和多样化的生态系统。 加勒比海的珊瑚礁是疾病热点，许多珊瑚礁由于疾病爆发而经历了人口崩溃。重要的是，珊瑚物种对疾病的易感性各不相同，但这种差异背后的原因尚不清楚。该项目将通过使用几种新的方法和实验来检查珊瑚的免疫力，从而量化珊瑚对疾病的易感性。将使用七种在疾病易感性、生长速度、生长形式和繁殖策略方面不同的珊瑚。每种珊瑚的免疫反应将通过将珊瑚暴露于细菌免疫刺激剂来测量。白色鼠疫是一种影响许多种类珊瑚的流行病，也将通过将珊瑚暴露于活跃的白色鼠疫并计算疾病传播率来测量对这种疾病的易感性。每个珊瑚物种的免疫反应和疾病传播数据将用于开发预测模型，以确定不同的珊瑚群落在气候变化情景下将如何应对疾病威胁。该项目将支助得克萨斯大学、阿灵顿（西班牙裔服务机构）和维尔京群岛大学（历史黑人大学）的研究生以及所有三个机构（莫特海洋实验室）的许多本科生。这项研究将在所有三个机构定期举行的外展活动中得到强调，包括德克萨斯州达拉斯的地球日德克萨斯州，佛罗里达州萨拉索塔的Mote's Living Reef Exhibit and Aquarium以及美属维尔京群岛的“Reef Fest”和农业博览会。导致一些地区的人口锐减。然而，并非所有珊瑚物种，甚至物种内的种群，都受到疾病的影响。有些物种是许多不同类型疾病的宿主，但死亡率有限。其他物种则因疾病而死亡。如何和为什么疾病的易感性不同物种和这种差异的易感性对珊瑚礁群落结构和组成的影响目前还不清楚。该项目将使用免疫挑战实验，量化珊瑚先天免疫系统的新成分，再加上基于特征的模型的应用，以实现三个目标：1）确定加勒比海珊瑚礁上发现的七种常见珊瑚物种的珊瑚免疫特征的变异性，2）确定同一种珊瑚对白色鼠疫传播的抵抗力的变异性3）开发一个预测模型的珊瑚群落组合，结合免疫性状。珊瑚免疫力的定量还将采用独特的方法，例如将全转录组测序与蛋白质活性测定相结合，进行基因-表型分析。数据将被映射到免疫途径上，用于珊瑚物种之间的综合途径评价，这些数据将作为“特征空间”模型的特征输入。这些性状将在模型中提供连续数据，这将为每个物种的性状分布创建概率密度函数（PDF）。然后，这些PDF将用于确定不同疾病暴露情景下的物种概率。模型分析将确定哪些特征影响群落结构，并描述疾病暴露和免疫反应如何通过空间和时间预测群落组合。该项目的一个独特产品是完成和应用一个包含广泛免疫参数的基于特征的模型（其中没有一个应用于珊瑚生态系统内的特征模型）。

项目成果

Deciphering Coral Disease Dynamics: Integrating Host, Microbiome, and the Changing Environment

• DOI: 10.3389/fevo.2020.575927
• 发表时间: 2020-11
• 作者: Rebecca L. Vega Thurber;L. Mydlarz;M. Brandt;D. Harvell;E. Weil;L. Raymundo;B. Willis;Stanley L Langevin;Allison M. Tracy;Raechel A. Littman;Keri M Kemp;P. Dawkins;K. Prager;M. Garren;J. Lamb

Responding to Threats Both Foreign and Domestic: NOD-Like Receptors in Corals

• DOI: 10.1093/icb/icz111
• 发表时间: 2019-10-01
• 期刊: INTEGRATIVE AND COMPARATIVE BIOLOGY
• 影响因子: 2.6
• 作者: Dimos, Bradford A.;Butler, Caleb C.;Mydlarz, Laura D.
• 通讯作者: Mydlarz, Laura D.

Life or death: disease-tolerant coral species activate autophagy following immune challenge

• DOI: 10.1098/rspb.2017.0771
• 发表时间: 2017-06-14
• 期刊: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
• 影响因子: 4.7
• 作者: Fuess, Lauren E.;Pinzon, Jorge H. C.;Mydlarz, Laura D.
• 通讯作者: Mydlarz, Laura D.

The effects of environmental history and thermal stress on coral physiology and immunity

环境史和热应激对珊瑚生理和免疫的影响

• DOI: 10.1007/s00227-018-3317-z
• 发表时间: 2018
• 期刊: Marine Biology
• 影响因子: 2.4
• 作者: Wall, Christopher B.;Ricci, Contessa A.;Foulds, Grace E.;Mydlarz, Laura D.;Gates, Ruth D.;Putnam, Hollie M.
• 通讯作者: Putnam, Hollie M.

Investigating the roles of transforming growth factor-beta in immune response of Orbicella faveolata, a scleractinian coral

• DOI: 10.1016/j.dci.2020.103639
• 发表时间: 2020-06-01
• 期刊: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
• 影响因子: 2.9
• 作者: Fuess, Lauren E.;Butler, Caleb C.;Mydlarz, Laura D.
• 通讯作者: Mydlarz, Laura D.