权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Collaborative Research: Examining the role of nitrogen exchange in the formation of algal blooms

合作研究：研究氮交换在藻华形成中的作用

基本信息

批准号：
1715909
负责人：
Silvia Newell
金额：
$ 15万
依托单位：
Wright State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-15 至 2021-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1715909&HistoricalAwards=false
关键词：
Collaborative Research Examining role nitrogen

项目摘要

Cyanobacterial algal blooms plague lakes and rivers across the globe. These toxic blooms damage the health of lake ecosystems, threaten the public health of surrounding communities, and harm local economies due a reduction in tourism and fishing industries. Blooms are hard to combat, mainly because the factors that contribute to the successful growth of bloom-forming microbes are not yet fully understood. This project will provide new insight into how algal blooms occur by focusing on how the algae interact with surrounding bacteria to obtain and exchange the nutrients, especially nitrogen, that allow the blooms to occur. Through a combination of experimental manipulation, measurements, and physiological modeling, the work will provide a detailed picture of how nitrogen exchange promotes algal blooms. This results will help environmental managers develop effective mitigation strategies to combat toxic blooms in lakes and rivers. Experiments will be conducted by undergraduate students at James Madison and Wright State Universities, and much of the research will take place in an undergraduate classroom setting, providing excellent training for students early in their science careers. The researchers will also work with homeschooled students in rural areas, inspiring potential young scientists.In aquatic systems, interactions between primary producers and heterotrophic bacteria alter water chemistry and shape ecosystem function. The goal of this project is to quantify and model interactions, specifically nitrogen exchange, between heterotrophic bacteria and a model primary producer in the phycosphere (the microenvironment surrounding a phytoplankton cell). This work will: 1) quantify the reliance of the model freshwater cyanobacterium Microcystis on the heterotrophic bacteria in the phycosphere; 2) quantify and model the transfer of nitrogen between Microcystis and specific phycosphere partners, using co-culture experiments with a single heterotrophic partner, and profile the transcriptome while simultaneously performing targeted nitrogen-related metabolite analysis; and 3) expand the model of nitrogen transfer to the entire phycosphere consortium by tracing and quantifying the flow of different nitrogen compounds through the symbiotic phycosphere consortium.

蓝藻水华困扰着地球仪的湖泊和河流。这些有毒的水华破坏了湖泊生态系统的健康，威胁到周围社区的公共健康，并由于旅游业和渔业的减少而损害了当地经济。水华很难对付，主要是因为促成水华形成微生物成功生长的因素尚未完全了解。该项目将通过关注藻类如何与周围的细菌相互作用以获得和交换允许水华发生的营养物质，特别是氮，来提供对藻类水华如何发生的新见解。通过实验操作，测量和生理建模的结合，这项工作将提供氮交换如何促进藻类水华的详细图片。这一结果将有助于环境管理人员制定有效的缓解战略，以打击湖泊和河流中的有毒水华。实验将由詹姆斯麦迪逊和赖特州立大学的本科生进行，大部分研究将在本科课堂环境中进行，为学生在科学生涯早期提供出色的培训。研究人员还将与农村地区的在家上学的学生合作，激发潜在的年轻科学家。在水生系统中，初级生产者和异养细菌之间的相互作用改变了水化学，塑造了生态系统功能。该项目的目标是量化和建模异养细菌和藻圈（浮游植物细胞周围的微环境）中模型初级生产者之间的相互作用，特别是氮交换。这项工作将：2）使用与单一异养伴侣的共培养实验，对微囊藻和特定藻圈伴侣之间的氮转移进行量化和建模，并在同时进行靶向氮相关代谢物分析的同时对转录组进行分析;和3）通过追踪和量化不同氮化合物通过共生藻球聚生体的流动，将氮转移模型扩展到整个藻球聚生体。