Collaborative Research: Examining the role of nitrogen exchange in the formation of algal blooms

合作研究：研究氮交换在藻华形成中的作用

基本信息

批准号：
1715909
负责人：
Silvia Newell
金额：
$ 15万
依托单位：
Wright State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-15 至 2021-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1715909&HistoricalAwards=false
关键词：
Collaborative Research Examining role nitrogen

项目摘要

Cyanobacterial algal blooms plague lakes and rivers across the globe. These toxic blooms damage the health of lake ecosystems, threaten the public health of surrounding communities, and harm local economies due a reduction in tourism and fishing industries. Blooms are hard to combat, mainly because the factors that contribute to the successful growth of bloom-forming microbes are not yet fully understood. This project will provide new insight into how algal blooms occur by focusing on how the algae interact with surrounding bacteria to obtain and exchange the nutrients, especially nitrogen, that allow the blooms to occur. Through a combination of experimental manipulation, measurements, and physiological modeling, the work will provide a detailed picture of how nitrogen exchange promotes algal blooms. This results will help environmental managers develop effective mitigation strategies to combat toxic blooms in lakes and rivers. Experiments will be conducted by undergraduate students at James Madison and Wright State Universities, and much of the research will take place in an undergraduate classroom setting, providing excellent training for students early in their science careers. The researchers will also work with homeschooled students in rural areas, inspiring potential young scientists.In aquatic systems, interactions between primary producers and heterotrophic bacteria alter water chemistry and shape ecosystem function. The goal of this project is to quantify and model interactions, specifically nitrogen exchange, between heterotrophic bacteria and a model primary producer in the phycosphere (the microenvironment surrounding a phytoplankton cell). This work will: 1) quantify the reliance of the model freshwater cyanobacterium Microcystis on the heterotrophic bacteria in the phycosphere; 2) quantify and model the transfer of nitrogen between Microcystis and specific phycosphere partners, using co-culture experiments with a single heterotrophic partner, and profile the transcriptome while simultaneously performing targeted nitrogen-related metabolite analysis; and 3) expand the model of nitrogen transfer to the entire phycosphere consortium by tracing and quantifying the flow of different nitrogen compounds through the symbiotic phycosphere consortium.

蓝细菌藻类在全球盛开鼠疫湖泊和河流。这些有毒的开花会损害湖泊生态系统的健康，威胁到周围社区的公共卫生，并因旅游业和捕鱼行业的减少而损害当地经济。盛开很难打击，主要是因为尚未完全了解促进花朵形成微生物的成功增长的因素。该项目将通过关注藻类如何与周围细菌相互作用，以获取和交换营养，尤其是氮，从而使藻类如何相互作用，从而提供新的见解，以使藻类发生。通过实验操作，测量和生理建模的结合，这项工作将提供详细的氮交换方式促进藻华的详细图片。该结果将有助于环境经理制定有效的缓解策略，以打击湖泊和河流中的有毒花朵。实验将由詹姆斯·麦迪逊（James Madison）和赖特州立大学（Wright State Universities）的本科生进行，大部分研究将在本科课堂环境中进行，为学生早期的科学职业提供出色的培训。研究人员还将与农村地区的家庭学习学生合作，激发潜在的年轻科学家。在水生系统中，主要生产者与异养细菌之间的相互作用改变了水化学的化学和形状生态系统功能。该项目的目的是量化和建模异构细菌和模型的主要生产者（围绕浮游植物细胞的微环境）之间的模型主要生产者之间的相互作用，特别是氮交换。这项工作将：1）量化模型的淡水蓝细胞微囊藻对卵圈中异养细菌的依赖； 2）使用与单个异养伴侣的共培养实验对微囊藻和特定卵圈伴侣之间的氮转移进行量化和建模，同时介绍了转录组，同时同时执行靶向氮相关的代谢物分析； 3）通过追踪和量化通过共生植物圈联盟的不同氮化合物的流动，将氮转移的模型扩展到整个植物圈联盟。