MRI: Acquisition of an ultra performance liquid chromatography mass spectrometer (UPLC-MS) for multi-disciplinary research

MRI：购买超高效液相色谱质谱仪 (UPLC-MS) 用于多学科研究

• 批准号: 1726903
• 负责人: Paula Hudson
• 金额: $ 36.82万
• 依托单位: CSU Fullerton Auxiliary Services Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726903&HistoricalAwards=false
• 关键词: MRI; Acquisition; ultra; performance; liquid

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation (CRIF) Programs. Professor Paula Hudson from California State University Fullerton and colleagues Madeline Rasche, Sudarshan Kurwadkar, Nicholas Salzameda and Alexandra Orchard have acquired a benchtop quadrupole, liquid chromatograph tandem mass spectrometer (Q-LCMS/MS.) In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized into ions and the ions' masses are measured. This highly sensitive technique allows the structure of molecules in a complex mixtures to be studied. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse groups of students in research and research training using this modern analytical technique. It also provides training opportunities to many undergraduate students at this Hispanic-serving institution since they are the major workforce in research laboratories at CSUF. Additionally, research programs on the CSUF campus engage students from local community colleges through ten and twelve-week summer research experiences supported through the Regional Alliance in STEM Education (Project RAISE).The mass spectrometer enhances research and education at all levels. This instrument especially impacts research on identification and quantification of atmospherically and environmentally relevant molecules. It is used in rapidly characterizing ,with high accuracy, inhibitors of protein-protein interactions of enzymes related to human health and disease. The spectrometer is also used in designing enzyme assays to identify small molecule protease inhibitors and quantify small molecules used in the discovery and kinetic characterization of enzyme catalysts.

该奖项由主要研究仪器 (MRI) 和化学研究仪器 (CRIF) 项目支持。加州州立大学富勒顿分校的 Paula Hudson 教授及其同事 Madeline Rasche、Sudarshan Kurwadkar、Nicholas Salzameda 和 Alexandra Orchard 购买了一台台式四极杆液相色谱串联质谱仪 (Q-LCMS/MS)。一般来说，质谱 (MS) 是用于识别和表征少量化学品的关键分析方法之一 嵌入复杂样本中的物种。在典型的实验中，成分流入质谱仪，在那里它们被电离成离子，并测量离子的质量。这种高度灵敏的技术可以研究复杂混合物中的分子结构。带有液相色谱仪的仪器可以在化合物混合物到达质谱仪之前对其进行分离。 此次收购加强了大学和地区的研究基础设施。该仪器通过使用这种现代分析技术让不同的学生群体参与研究和研究培训来扩大参与范围。它还为这所为西班牙裔服务的机构的许多本科生提供培训机会，因为他们是 CSUF 研究实验室的主要劳动力。此外，CSUF 校园的研究项目通过 STEM 教育区域联盟（RAISE 项目）的支持，吸引当地社区学院的学生参加为期 10 周和 12 周的夏季研究体验。质谱仪增强了各个级别的研究和教育。该仪器尤其影响大气和环境相关分子的识别和定量研究。 它用于快速、高精度地表征与人类健康和疾病相关的酶的蛋白质-蛋白质相互作用的抑制剂。该光谱仪还用于设计酶测定，以识别小分子蛋白酶抑制剂并量化用于酶催化剂发现和动力学表征的小分子。

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