Laser-Scanning Mikroskop mit konfokaler Optik

具有共焦光学器件的激光扫描显微镜

• 批准号: 231039605
• 金额: --
• 依托单位: Ruprecht-Karls-Universität Heidelberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/231039605?language=en
• 关键词: Laser; Scanning; Mikroskop; mit; konfokaler

We study the regulatory mechanisms underlying plant stem cell homeostasis at the shoot apical meristem. Since the stem cell population itself as well as the cells providing the organizational cues for meristem integrity undergo permanent turnover, we need to expand our studies from the static 2D level afforded by histological sections to the dynamic 4D level achieved by time lapse confocal microscopy. We have developed plant lines carrying multi-color fluorescent reporters, which will enable us to visualize all major domains within the shoot apical meristem simultaneously. Since the reporters are targeted to the nucleus, it will be possible to quantify the cell number within a given domain. In addition we are developing powerful markers to assess cell division activity and together with cell identity markers, these tools will give us unprecedented insight into cell behavior in the apical stem cell niche of Arabidopsis. For these experiments we need access to a versatile upright confocal microscope able to resolve spectral information due to the multitude of simultaneously detected markers for many hours for each experiment. Furthermore, to acquire quantitative information, we will need a substantial level of automation for imaging. So far, an instrument that meets these requirements does not exist at our institution and the available instruments are highly used precluding these types of analysis.

我们研究了茎尖分生组织植物干细胞稳态的调节机制。由于干细胞群本身以及为分生组织完整性提供组织线索的细胞会经历永久更新，因此我们需要将我们的研究从组织学切片提供的静态 2D 水平扩展到延时共聚焦显微镜实现的动态 4D 水平。我们开发了携带多色荧光报告基因的植物品系，这将使我们能够同时可视化茎尖分生组织内的所有主要区域。由于报告基因针对细胞核，因此可以量化给定域内的细胞数量。此外，我们正在开发强大的标记来评估细胞分裂活性，并与细胞身份标记一起使用，这些工​​具将为我们提供对拟南芥顶端干细胞生态位中细胞行为的前所未有的了解。对于这些实验，我们需要使用多功能的直立共焦显微镜，该显微镜能够解析光谱信息，因为每个实验需要多个小时同时检测到大量标记。此外，为了获取定量信息，我们需要高水平的成像自动化。到目前为止，我们的机构还不存在满足这些要求的仪器，并且可用的仪器被广泛使用，排除了这些类型的分析。

项目成果

Live imaging of Arabidopsis development.

• DOI: 10.1007/978-1-62703-580-4_28
• 发表时间: 2014
• 期刊: Methods in molecular biology
• 作者: Daniel von Wangenheim;Gabor Daum;J. Lohmann;Ernst H. K. Stelzer;A. Maizel

A mechanistic framework for noncell autonomous stem cell induction in Arabidopsis

• DOI: 10.1073/pnas.1406446111
• 发表时间: 2014-10-07
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Daum, Gabor;Medzihradszky, Anna;Lohmann, Jan U.
• 通讯作者: Lohmann, Jan U.

Integration of light and metabolic signals for stem cell activation at the shoot apical meristem

• DOI: 10.7554/elife.17023
• 发表时间: 2016-07-11
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Pfeiffer, Anne;Janocha, Denis;Lohmann, Jan U.
• 通讯作者: Lohmann, Jan U.

Job Sharing in the Endomembrane System: Vacuolar Acidification Requires the Combined Activity of V-ATPase and V-PPase

• DOI: 10.1105/tpc.15.00733
• 发表时间: 2015-12-01
• 期刊: PLANT CELL
• 影响因子: 11.6
• 作者: Kriegel, Anne;Andres, Zaida;Schumacher, Karin
• 通讯作者: Schumacher, Karin