权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Collaborative Research: Linking environmental challenges to the likelihood and severity of epidemics: A view through the shifting window of susceptibility

合作研究：将环境挑战与流行病的可能性和严重程度联系起来：通过易感性窗口的变化观察

基本信息

批准号：
1754474
负责人：
Jesse Brunner
金额：
$ 70万
依托单位：
Washington State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-15 至 2024-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754474&HistoricalAwards=false
关键词：
Collaborative Research Linking environmental challenges

项目摘要

This research will help biologists who manage wildlife populations better understand the environmental conditions that make disease outbreaks more likely to catch hold and spread. A fundamental goal in biology is understanding how the environment affects human and wildlife health. Stressful environments, for instance, can make animals more susceptible to infection. But it is difficult to predict or manage the effects of stress on disease outbreaks because there are many complex, interacting processes. In addition, individuals may not respond the same way to stress. This research project will test whether chronic stress weakens the immune system during key times in an animal's development termed 'critical windows' of susceptibility. It will test whether animals are more easily infected, better at spreading the infection, and more likely to die. These results will then be used to test whether environmental stressors make disease outbreaks more likely and severe for whole populations. This work will help prioritize actions to prevent or lessen disease outbreaks. It will also provide a more sophisticated framework for studying animal diseases. In addition, the researchers will develop new teaching modules and workshops to help inform state, federal, and academic scientists, land managers, teachers, and college students about the ways in which stress affects disease outcomes. The projects will use a ranavirus, an often-lethal pathogen of amphibians, and their wood frog tadpole hosts as model system to test three specific hypotheses: environmental stressors 1) increase the average susceptibility of hosts, 2) change the timing and duration of critical windows of susceptibility, and 3) magnify initially small differences in susceptibility among hosts. A series of dose-response experiments will characterize the windows and distribution of susceptibility (both resistance to and tolerance of ranavirus infections) and determine how they are affected by high salinity and elevated temperature - two relevant and increasingly important environmental challenges. Second, laboratory experiments will characterize how these stressors affect ranavirus transmission. It is hypothesized that the most susceptible tadpoles will also be most infectious. Third, the specific physiological and immunological responses will be measured in the most and least susceptible individuals to better understand the mechanisms underlying these responses. Fourth, a sophisticated mathematical model will be used to translate these results to population-level outcomes. Lastly, experimental epidemics in semi-natural populations will be used to test the model's predictions of how stressors alter critical windows of susceptibility to affect the likelihood and severity of ranavirus epidemics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项研究将帮助管理野生动物种群的生物学家更好地了解使疾病爆发更容易控制和传播的环境条件。生物学的一个基本目标是了解环境如何影响人类和野生动物的健康。例如，紧张的环境会使动物更容易受到感染。但是很难预测或管理压力对疾病爆发的影响，因为有许多复杂的相互作用的过程。此外，个体对压力的反应可能不一样。这项研究项目将测试慢性压力是否会在动物发育的关键时期削弱免疫系统，称为易感性的“关键窗口”。它将测试动物是否更容易感染，更容易传播感染，更容易死亡。这些结果将用于测试环境压力是否会使整个人群更有可能和更严重地爆发疾病。这项工作将有助于确定预防或减少疾病爆发的优先行动。它还将为研究动物疾病提供一个更复杂的框架。此外，研究人员将开发新的教学模块和研讨会，以帮助告知州，联邦和学术科学家，土地管理者，教师和大学生关于压力影响疾病结果的方式。这些项目将使用蛙病毒，一种两栖动物的致命病原体，以及它们的林蛙蝌蚪宿主作为模型系统来测试三个特定的假设：环境压力1）增加宿主的平均易感性，2）改变易感性临界窗口的时间和持续时间，以及3）放大宿主之间易感性的最初微小差异。一系列剂量反应实验将表征敏感性的窗口和分布（对蛙病毒感染的抗性和耐受性），并确定它们如何受到高盐度和高温的影响-这两个相关且日益重要的环境挑战。其次，实验室实验将描述这些压力源如何影响Ranavirus传播。据推测，最易感的蝌蚪也将是最具传染性的。第三，将在最敏感和最不敏感的个体中测量特定的生理和免疫反应，以更好地理解这些反应的机制。第四，将使用一个复杂的数学模型将这些结果转化为人口一级的结果。最后，半自然人群中的实验性流行病将用于测试模型对压力源如何改变易感性的关键窗口以影响Ranavirus流行病的可能性和严重程度的预测。该奖项反映了NSF的法定使命，并且通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。