Community processes structuring assembly and disassembly of bat gut-microbial communities across a gradient of habitat degradation

蝙蝠肠道微生物群落在栖息地退化梯度中构建组装和分解的群落过程

• 批准号: 1754810
• 负责人: Tigga Kingston
• 金额: $ 83万
• 依托单位: Texas Tech University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-06-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754810&HistoricalAwards=false
• 关键词: Community; processes; structuring; assembly; disassembly

Mammal guts are home to communities of microbes that play an important role in the nutrition and health of individual animals. These microbial communities, or microbiomes, are made up of hundreds of species of bacteria, but which species are present, and how common they are, varies from individual to individual animal. This variability is important because abnormal communities can lead to conditions that range from obesity to depression. However, the rules by which microbial communities come together or breakdown are poorly understood. Using wild bats as models, the researchers will investigate how host sociality, diet, genetics, and microbe functions and interactions shape the make-up of digestive microbiomes. The results of this research to understand the rules governing microbiome composition can help advance human and wildlife health. To improve students' understanding of how scientific research is conducted, the team will engage elementary school students from around the country in the project, sharing data live from the field in real time. Students will learn how to analyze and interpret the project data through the researchers' STEM K-12 initiative: The Malaysian Bat Education Adventure. Using an existing Biodiversity module (grades 5-6) and a new module "Microbial Diversity: What's in your bat?" (grades 6-7), students will be introduced to topics of microbial ecology and diversity. The program's success in changing student STEM knowledge, skills and attitude will be evaluated with quantitative methods in a pre- and post- intervention design with control group.The research will determine the relative role of community processes in structuring gut-microbial composition and function in insectivorous bat species co-distributed across a gradient of habitat degradation. The researchers hypothesize that microbiome community assembly and disassembly involve deterministic processes of niche filtering, species interactions, and dispersal. This research will test the hypothesis that changes in host ecology wrought by habitat degradation will alter the relative influence of these processes with consequences for community composition and function. Bats will be studied across a forest modification gradient in Malaysia, and individually-matched samples of gut-microbial composition, metagenome function, dietary composition, host genotype and measures of social interaction obtained for each bat. These data, in conjunction with analyses of co-occurrence, phylogenetic and functional trait patterns, will be used to test structural equation models specified to untangle the processes structuring communities in a causal modeling framework. Evidence of the role of dispersal in structuring microbiomes will derive from comparisons of co-distributed host species that differ in sociality. Interspecific associations will be assessed using co-occurrence network approaches. Functional trait space is developed from genomic inference for each bacterial phylotype within an individual bat's microbiome. Phylogenies are estimated using maximum likelihood and 16S gene alignment among community members. To test the role of diet and host genetics (the host environment) in community processes, the researchers describe diet from feces with the COI DNA barcodes, and quantify host diversity and relatedness through genome fingerprinting.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

哺乳动物的肠道是微生物群落的家园，这些微生物在个体动物的营养和健康方面发挥着重要作用。这些微生物群落或微生物组由数百种细菌组成，但存在哪些物种以及它们的常见程度因个体而异。这种变异性很重要，因为不正常的社区可能导致从肥胖到抑郁症的各种疾病。然而，微生物群落聚集或分解的规则却知之甚少。以野生蝙蝠为模型，研究人员将研究宿主的社会性、饮食、遗传学和微生物功能和相互作用如何塑造消化微生物组的构成。 这项旨在了解微生物组组成规则的研究结果有助于促进人类和野生动物的健康。为了提高学生对科学研究如何进行的理解，该团队将让来自全国各地的小学生参与该项目，以真实的时间分享现场数据。学生将学习如何通过研究人员的STEM K-12计划分析和解释项目数据：马来西亚蝙蝠教育冒险。 使用现有的生物多样性模块（等级5-6）和新模块“微生物多样性：你的蝙蝠？“（6-7年级），学生将了解微生物生态学和多样性的主题。该项目在改变学生STEM知识、技能和态度方面的成功将通过干预前后的对照组设计，用定量方法进行评估。该研究将确定群落过程在构建肠道微生物组成和功能中的相对作用，以及共同分布在栖息地退化梯度上的食虫蝙蝠物种的功能。研究人员假设微生物群落的组装和拆卸涉及生态位过滤，物种相互作用和扩散的确定性过程。这项研究将测试的假设，在宿主生态的变化所造成的栖息地退化将改变这些过程的相对影响，社区的组成和功能的后果。蝙蝠将在马来西亚的森林改造梯度进行研究，并为每只蝙蝠获得肠道微生物组成，宏基因组功能，饮食组成，宿主基因型和社会互动措施的个体匹配样本。这些数据，结合共现，系统发育和功能性状模式的分析，将被用来测试结构方程模型，指定解开的过程结构社区的因果建模框架。分散在构建微生物组中的作用的证据将来自于不同社会性的共分布宿主物种的比较。将使用共现网络方法评估种间关联。功能性状空间是从个体蝙蝠微生物组内每种细菌的基因组推断中开发出来的。系统发育估计使用最大似然法和16 S基因比对之间的社区成员。为了测试饮食和宿主遗传学（宿主环境）在群落过程中的作用，研究人员用COI DNA条形码描述粪便中的饮食，并通过基因组指纹分析量化宿主多样性和相关性。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。